Expression of nitric oxide synthase isoforms in different stages of buffalo (Bubalus bubalis) ovarian follicles: Effect of nitric oxide on in vitro development of preantral follicle

Dubey, Pawan K.; Tripathi, Vishal; Singh, Ram Pratap; Saikumar, G.; Nath, Amar; Pratheesh,; Gade, Nitin E.; Sharma, G. Taru

doi:10.1016/j.theriogenology.2011.08.002

Cited by 31 publications

(19 citation statements)

References 27 publications

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“…Evidence from mouse, cow, pig, and buffalo embryos, demonstrating that hemoglobin addition aids development during embryo culture, is varied [38][39][40][41][42][43]. As far as we know, we are the first to examine the supplementation of hemoglobin during IVM, based on the observations that IVM-derived oocytes lacked cytoplasmic hemoglobin, unlike their in vivo counterparts.…”

Section: Discussionmentioning

confidence: 99%

Hemoglobin: a Gas Transport Molecule That Is Hormonally Regulated in the Ovarian Follicle in Mice and Humans1

Brown

Anastasi

Frank

et al. 2015

Biology of Reproduction

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An increasing number of nonerythroid tissues are found to express hemoglobin mRNA and protein. Hemoglobin is a well-described gas transport molecule, especially for O2, but also for NO, CO2, and CO, and also acts as a reactive oxygen species scavenger. We previously found Hba-a1 and Hbb mRNA and protein at high levels within mouse periovulatory cumulus cells, but not in cumulus following in vitro maturation. This led us to investigate the temporal and spatial regulation in follicular cells during the periovulatory period. Cumulus-oocyte complexes were collected from equine chorionic gonadotropin/human chorionic gonadotropin-treated peripubertal SV129 female mice and collected and analyzed for gene expression and protein localization at a variety of time points over the periovulatory period. A further cohort matured in vitro with different forms of hemoglobin (ferro- and ferrihemoglobin) under different O2 atmospheric conditions (2%, 5%, and 20% O2) were subsequently fertilized in vitro and cultured to the blastocyst stage. Murine mRNA transcripts for hemoglobin were regulated by stimulation of the ovulatory cascade, in both granulosa and cumulus cells, and expression of HBA1 and HBB was highly significant in human granulosa and cumulus, but erythrocyte cell marker genes were not. Several other genes involved in hemoglobin function were similarly luteinizing hormone-regulated, including genes for heme biosynthesis. Immunohistochemistry revealed a changing localization pattern of HBA-A1 protein in murine cumulus cells and oocytes following the ovulatory signal. Significantly, no positive staining for HBA-A1 protein was observed within in vitro-matured oocytes, but, if coincubated with ferro- or ferrihemoglobin, cytoplasmic HBA-A1 was observed, similar to in vivo-derived oocytes. Addition of ferro-, but not ferrihemoglobin, had a small, positive effect on blastocyst yield, but only under either 2% or 20% O2 gas atmosphere. The identification of hemoglobin within granulosa and cumulus cells poses many questions as to its function in these cells. There are several possible roles, the most likely of which is either an O2 or NO sequestering molecule; perhaps both roles are engaged. The strong endocrine regulation during the periovulatory period suggests to us that one potential function of hemoglobin is to provide a short-lived hypoxic environment by binding very tightly any available O2. This, in turn, facilitates the differentiation of the follicle towards corpus luteum formation by enabling the stabilization of a key transcription factor known to initiate such differentiation: hypoxia inducible factor.

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Section: Discussionmentioning

confidence: 99%

Hemoglobin: a Gas Transport Molecule That Is Hormonally Regulated in the Ovarian Follicle in Mice and Humans1

Brown

Anastasi

Frank

et al. 2015

Biology of Reproduction

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“…To confirm the integrity of the mRNA templates and RT-PCR protocol, the housekeeping gene b-actin was examined in all samples. The estimation of mRNA level in different stages of follicles was carried out according to Dubey et al (21). To control the variation in the efficiencies of the RT step among different experimental samples, band density of b-actin, a housekeeping gene, presumed to be expressed at constant amounts in follicles were also calculated, along with band density of targeted genes, by densitometry analysis using ImageJ 1.43U software (NIH, Bethesda, Maryland).…”

Section: Quantification Of Type-i and II Igf-receptors Igf-i Ii Anmentioning

confidence: 99%

Expression of mRNA Encoding IGF-I, IGF-II, Type-I, and II IGF-Receptors and IGF-Binding Proteins-1-4 during Ovarian Follicular Development in Buffalo(Bubalus bubalis)

Dubey

Nath

Chandra

et al. 2014

Animal Biotechnology

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The present study was designed to investigate the expression pattern of IGF-I, IGF-II, type-I and II IGF-receptors, and IGFBP-1-4 in different stages of buffalo ovarian preantral follicles (PFs), antral follicles (AFs), ovulatory follicles (OFs), and immature (IM) and in vitro matured (MO) oocytes. Buffalo ovaries were collected from local abattoir, PFs (200-250 µm), AFs (1-3 mm), and OFs (5-8 mm) were isolated by mechanical method. PFs, AFs, OFs, and oocytes were lysed to release mRNA, reverse transcribed, and then subjected to RT-PCR, whereas protein were localized through immunohistochemistry. Relative expression of mRNA transcripts was clearly seen for IGF-II, type-I and II IGF-receptors, and IGFBP-1-4 in all the stages of developing follicles and oocytes. We were unable to detect mRNA and protein expression of IGF-1 in any of the oocytes or follicles at any stage of the development. IGF-II and both IGF receptors mRNA expression were found higher (P < 0.05) in PFs compared to AFs and OFs. Expression of IGFBP-1 and 2 in PFs, as well as IGFBP-3 and 4 in AFs, was found with higher (P < 0.05) levels. The expression results were further confirmed by localization of IGF-II, type-I and II IGF-receptors, and IGFBP-1-4 proteins. In conclusion, IGF-II appears to be the only ligand that is endogenously expressed by all the follicular stages and oocytes, which may act in an autocrine manner through the Type-1 IGF receptor. Expression of IGFBP-1-4 and IGF-II suggests the possible role of these genes in recruitment, growth, proliferation, and steroidogenic responses during developmental phases of buffalo ovarian follicles.

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“…RNS, on the other side, are necessary for the development of large antral follicles [17,18], to stimulate meiotic maturation in oocytes [19,20], in the ovulatory process [21], in early folliculogenesis up to the maturation step [22] and in preimplantation embryonic development [23,24]. Additionally, RNS participate in sperm capacitation [25,26,27] and the acrosome reaction [28].…”

Section: Introductionmentioning

confidence: 99%

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on Gamete Handling and In Vitro Embryo Production in Humans and Other Mammals

Loren

Sánchez

Arias

et al. 2017

IJMS

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Oxidative and nitrosative stress are common problems when handling gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because their absence or overproduction causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during in vitro fertilization (IVF) has deleterious or positive effects, depending on the concentration used in the culture medium, demonstrating the delicate balance between antioxidant and pro-oxidant activity. Further research is needed to better understand the possible impact of melatonin on the different IVP steps in humans and other mammals, especially in seasonal breeds where this neuro-hormone system highly regulates its reproduction physiology.

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Expression of nitric oxide synthase isoforms in different stages of buffalo (Bubalus bubalis) ovarian follicles: Effect of nitric oxide on in vitro development of preantral follicle

Cited by 31 publications

References 27 publications

Hemoglobin: a Gas Transport Molecule That Is Hormonally Regulated in the Ovarian Follicle in Mice and Humans1

Hemoglobin: a Gas Transport Molecule That Is Hormonally Regulated in the Ovarian Follicle in Mice and Humans1

Expression of mRNA Encoding IGF-I, IGF-II, Type-I, and II IGF-Receptors and IGF-Binding Proteins-1-4 during Ovarian Follicular Development in Buffalo(Bubalus bubalis)

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on Gamete Handling and In Vitro Embryo Production in Humans and Other Mammals

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