Expression of NGF, Trka and p75 in human cartilage

Gigante, Antonio; Bevilacqua, Claudia; Pagnotta, Alessia; Manzotti, S; Toesca, Amelia; Greco, Francesco

doi:10.4081/844

Cited by 43 publications

(38 citation statements)

References 21 publications

(16 reference statements)

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“…Nevertheless, they are in accordance with findings that NGF and p75 are expressed in human articular chondrocytes [Gigante et al, 2003]. Furthermore, NGF mRNA was shown for isolated chondrocytes of 2 normal and 2 osteoarthritic human knee joints [Iannone et al, 2002].…”

Section: Reaction Patterns For the Articular Cartilagesupporting

confidence: 79%

Expression Patterns of Neurotrophins and Neurotrophin Receptors in Articular Chondrocytes and Inflammatory Infiltrates in Knee Joint Arthritis

Grimsholm¹,

Guo

et al. 2008

Cells Tissues Organs

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Background: It is likely that neurotrophins (NTs) are of great importance for the articular cartilage and the inflammation process in arthritis. Methods: The immunohistochemical expression of the NTs nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) and the associated receptors p75, TrkA and TrkB was examined in the knee joint of arthritic and healthy mice. Results: Immunoreactions for NGF and BDNF were detected in cells and nerve fiber varicosities in the inflammatory infiltrates of the synovial tissue of arthritic joints but not in synovial tissue of controls. p75-immunoreactive nerve fiber-like strands were detected in inflammatory infiltrates. Immunostaining for NGF, BDNF, p75, TrkA and TrkB was noted in articular chondrocytes. There was a statistically significant decrease in reactions for NGF (p < 0.001), TrkA (p = 0.001) and p75 (p < 0.001) in articular chondrocytes in joints exhibiting severe arthritis. Conclusion: The findings show that an NT system develops in inflammatory infiltrates of the synovial tissue. Furthermore, most interestingly, autocrine/paracrine effects appear to exist concerning NTs for the articular chondrocytes. The downregulated expression of NGF and NT receptors in articular chondrocytes in arthritis is a new aspect concerning the involvement of NTs in cartilage.

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Section: Reaction Patterns For the Articular Cartilagesupporting

confidence: 79%

Expression Patterns of Neurotrophins and Neurotrophin Receptors in Articular Chondrocytes and Inflammatory Infiltrates in Knee Joint Arthritis

Grimsholm¹,

Guo

et al. 2008

Cells Tissues Organs

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“…There are virtually no data about CD271 expression during chondrogenesis. However, data from Gigante et al [2003] support our findings, i.e. the expression of CD271 on chondrocytes in cartilage.…”

Section: Discussionsupporting

confidence: 46%

Isolation and Characterization of CD271<sup>+</sup> Stem Cells Derived from Sheep Dermal Skin

Jahroomishirazi¹,

Bader²,

Ebert³

et al. 2014

Cells Tissues Organs

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Background: Mesenchymal stem cells (MSCs) have great promise in the field of regenerative medicine due to their differentiation potential into several lineages. Besides the bone marrow, MSCs can be obtained from the dermis, which represents a large stem cell reservoir in the skin. Sheep provide an appropriate large animal model for preclinical studies. In this study, we focused on the isolation and characterization of MSCs from sheep dermis as an alternative to bone marrow MSCs (bmMSCs). Methods: Primary ovine cells were obtained from the dermis for comparison with bone marrow. CD271⁺/45^- dermal MSCs (CD271-dMSCs), which were sorted by flow cytometry, and plastic-adherent bmMSCs were examined for morphology, proliferation and senescence-associated β-galactosidase activity in both low and high oxygen conditions. CD271 expression on cultured cells was assessed by flow cytometry. Adipogenic and osteogenic potentials of CD271-dMSCs were evaluated by oil red O and von Kossa staining. Chondrogenic capacity of CD271-dMSCs and CD271⁺/CD45^- bone marrow cells (CD271-bmMSCs) was detected using immunohistochemistry and measurement of sulfated glycosaminoglycans. Results: The cell proliferation assay demonstrated no significant difference between CD271-dMSCs and bmMSCs under low oxygen conditions. Cultured CD271-dMSCs revealed much more CD271 expression compared to CD271-bmMSCs. CD271-dMSCs and CD271-bmMSCs showed basically similar expression of the cartilage-specific proteins aggrecan and collagen type II, although with a stronger staining in CD271-bmMSC-derived cultures. Remarkably, there was co-expression of CD271 and aggrecan during chondrogenic differentiation, suggesting an involvement of CD271 in chondrogenesis. Conclusion: Based on these findings, CD271-dMSCs might serve as an appropriate alternative cell source in preclinical research.

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“…27 In our study, because most of the NGF produced was secreted into the media and only a minor portion stayed in the cell layer, it is possible that the immunolocalization technique used did not provide information about secreted NGF. Further organ culture studies may prove useful in the detection of NGF under more physiologic conditions.…”

Section: Discussionmentioning

confidence: 84%

“…13 More recently, Gigante et al have reported that rounded chondrocyte-like cells in the AF were immunohistochemically positive for NGF. 27 One question remains to be answered: which cells produce NGF in IVD tissue?…”

mentioning

confidence: 99%

Proinflammatory Cytokines Stimulate the Expression of Nerve Growth Factor by Human Intervertebral Disc Cells

Abe¹,

Akeda²,

An³

et al. 2007

Spine

145

139

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Our results demonstrate, for the first time, that human AF and NP cells constitutively express NGF protein and mRNA, and that the proinflammatory cytokines IL-1beta and TNF-alpha stimulate the production of NGF. The precise role of NGF produced by IVD cells in the generation of discogenic pain or on the metabolism of IVD cells, especially under certain physiologic conditions in which cytokines are up-regulated, needs to be clarified in future experimentation.

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Expression of NGF, Trka and p75 in human cartilage

Cited by 43 publications

References 21 publications

Expression Patterns of Neurotrophins and Neurotrophin Receptors in Articular Chondrocytes and Inflammatory Infiltrates in Knee Joint Arthritis

Expression Patterns of Neurotrophins and Neurotrophin Receptors in Articular Chondrocytes and Inflammatory Infiltrates in Knee Joint Arthritis

Isolation and Characterization of CD271<sup>+</sup> Stem Cells Derived from Sheep Dermal Skin

Proinflammatory Cytokines Stimulate the Expression of Nerve Growth Factor by Human Intervertebral Disc Cells

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