Expression of NGF receptor and NGF receptor mRNA in the developing and adult rat retina

Carmignoto, Giorgio; Comelli, Maria Cristina; Candeo, Paola; Cavicchioli, L.; Qiao, Yan; Merighi, Adalberto; Maffei, Lamberto

doi:10.1016/0014-4886(91)90097-v

Cited by 105 publications

(64 citation statements)

References 42 publications

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“…Immunolocalization of the TrkA revealed positive staining of retinal pigment epithelium and Müller cells. This result is consistent with previous studies [10,11,12,13,14,15]. …”

Section: Discussionsupporting

confidence: 83%

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Sun

Xu²,

Wang³

et al. 2007

Ophthalmologica

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Purpose: To investigate the expression of nerve growth factor (NGF) and its receptor TrkA on the retina at different time points after retinal detachment (RD) and the protection effect of NGF in experimental RD. Methods: Sprague-Dawley rats were used as an RD animal model by injection of 0.1% sodium hyaluronate under the neurosensory retina. The expression of endogenous NGF and its receptor TrkA in the rat retina was detected using immunohistochemistry. The NGF (5 µg/eye) or phosphate-buffered saline were injected separately into vitreous every 4 days after the RD. The rat eyes were then observed at various time points. Morphologic changes were investigated by light microscopy. Retinal gliosis was detected by glial fibrillary acidic protein labeling. Results: The expression of endogenous NGF and TrkA was upregulated during RD procedure. Most of the NGF-treated retina had a well-organized structure. In NGF-treated RD eyes, the cells were still significantly more numerous than in phosphate-buffered-saline-treated retina. Glial fibrillary acidic protein labeling increased quickly after RD; the NGF-treated retina had less reactive gliosis than the control groups. Conclusions: Intravitreal injection of exogenous NGF can protect retinal cells from degeneration in experimental RD. It may exert its protective action by preventing the apoptosis of retinal cells after RD.

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“…Immunolocalization of the TrkA revealed positive staining of retinal pigment epithelium and Müller cells. This result is consistent with previous studies [10,11,12,13,14,15]. …”

Section: Discussionsupporting

confidence: 83%

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Sun

Xu²,

Wang³

et al. 2007

Ophthalmologica

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“…In agreement with this, our results showed prominent glial Müller cell activation, which was evident from immunostaining of GFAP and accumulation of proNGF in the diabetic retina and in high glucose-cultured Müller cells. In light of the fact that p75 NTR is expressed in Müller cells and RGCs [27][28][29], our finding that the neuroprotective effect of epicatechin in vivo was associated with blocking the increases in expression of proNGF and p75 NTR suggests a paracrine effect of glial cells on neurons as target tissue. A co-culture model of RGCs with condition media from high glucose-treated glial rMC-1 showed an approximately 2.2-fold increase in proNGF levels and a 56% reduction in NTR expression and proNGF levels, and did not significantly alter RGC viability (85%~of basal level).…”

Section: Discussionmentioning

confidence: 87%

“…NTR is expressed mainly in Müller cells and RGCs [27][28][29]. Immunolocalisation of p75 NTR showed increased levels in Müller cells and inner retinal layers in diabetic rat retinas compared with controls (Fig.…”

Section: Epicatechin Blocks Diabetes-induced P75mentioning

confidence: 99%

Epicatechin blocks pro-nerve growth factor (proNGF)-mediated retinal neurodegeneration via inhibition of p75 neurotrophin receptor proNGF expression in a rat model of diabetes

et al. 2010

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Aims/hypothesis Accumulation of pro-nerve growth factor (NGF), the pro form of NGF, has been detected in neurodegenerative diseases. However, the role of proNGF in the diabetic retina and the molecular mechanisms by which proNGF causes retinal neurodegeneration remain unknown. The aim of this study was to elucidate the role of proNGF in neuroglial activation and to examine the neuroprotective effects of epicatechin, a selective inhibitor of tyrosine nitration, in an experimental rat model of diabetes. Methods Expression of proNGF and its receptors was examined in retinas from streptozotocin-induced diabetic rats, and in retinal Müller and retinal ganglion cells (RGCs). RGC death was assessed by TUNEL and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays in diabetic retinas and cell culture. Nitrotyrosine was determined using Slot-blot. Activation of the tyrosine kinase A (TrkA) receptor and p38 mitogen-activated protein kinase (p38MAPK) was assessed by western blot.Results Diabetes-induced peroxynitrite impaired phosphorylation of TrkA-Y490 via tyrosine nitration, activated glial cells and increased expression of proNGF and its receptor, p75 neurotrophin receptor (p75 NTR apoptotic pathway in RGCs, leading to neuronal cell death. These effects were blocked by epicatechin, a safe dietary supplement, suggesting its potential therapeutic use in diabetic patients.Electronic supplementary material The online version of this article

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“…Second. recent results have shown that intraventricular administration of NGF in monocularly deprived kittens largely prevents loss of visual acuity of the deprived eye, as tested behaviourally (Maffei et al 1991 (Mitchell, Cyander & Movshon, 1977), far greater recovery can be obtained by the reverse suturing procedure which depresses activity of the originally non-deprived eye (Blakemore & Van Sluyters, 1974). In the present experiments the reestablishment of DE functional synapses suppressed by the effects of visual deprivation was also possible under normal binocular vision, provided that NGF was exogenously supplied.…”

Section: Effect Of Ngf On the Recovery After MDmentioning

confidence: 99%

“…The hypothesis that the OD shift of area 17 neurones towards the open eye in monocularly deprived animals is due to competition between the afferents from the two eyes for NGF implies that geniculo-cortical axonal terminals express the NGF receptor. Recent evidence indicate that NGF receptor mRNA encoding for the low-affinity NGF receptor, and related protein are indeed expressed in many visual system related nuclei of the rat, including the visual cortex (Yan & Johnson, 1989;Pioro & Cuello 1990;Carmignoto et al 1991). We recently obtained evidence that in the lateral geniculate nucleus of the rat the low-affinity NGF receptor is exclusively related to retinal ganglion cell axonal terminals and not to intrinsic projecting neurones (Carmignoto, Candeo, Comelli, Calderini & Maffei, 1990).…”

Section: Possible Role Of Ngtf On Visual Cortical Plasticitymentioning

confidence: 99%

Effects of nerve growth factor on neuronal plasticity of the kitten visual cortex.

Carmignoto

Canella

Candeo

et al. 1993

The Journal of Physiology

100

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SUMMARY1. The effect of intraventricular administration of nerve growth factor (NGF) by means of a cannula-minipump system was studied in kittens monocularly deprived during the critical period. The ocular dominance of area 17 neurones of NGF-treated and control kittens was determined by conventional extracellular recordings. The soma size of cells in A and Al laminae of the lateral geniculate nucleus (LGN) was also evaluated in Cresyl Violet preparations.2. Binocularly responsive neurones were found to be significantly more numerous in NGF-treated than in control kittens. The shrinkage of cells from the deprived LGN laminae normally observed in control kittens was prevented by NGF administration.3. Following an initial period of monocular deprivation (MD) kittens subsequently treated with NGF showed a substantial recovery of functional binocular connections.4. These findings indicate that the administration of NGF during the period of deprivation reduces the amblyopic effects of MD, while its administration to kittens with both eyes open following the initial deprivation promotes recovery of the deprived eye.5. Neurotrophic factors may contribute to the regulation of experience-dependent modifications of synaptic connectivity in the visual cortex.

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Expression of NGF receptor and NGF receptor mRNA in the developing and adult rat retina

Cited by 105 publications

References 42 publications

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Nerve Growth Factor Helps Protect Retina in Experimental Retinal Detachment

Epicatechin blocks pro-nerve growth factor (proNGF)-mediated retinal neurodegeneration via inhibition of p75 neurotrophin receptor proNGF expression in a rat model of diabetes

Effects of nerve growth factor on neuronal plasticity of the kitten visual cortex.

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