Expression of Neuropeptide Y of GIFT Tilapia (Oreochromis sp.) in Yeast Pichia Pastoris and Its Stimulatory Effects on Food Intake and Growth

Wang, Guang-Zhong; Sun, Caiyun; Lin, Haoran; Li, Wensheng

doi:10.5772/17497

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…The ORF of NPYb, PYYa, and PYYb were successfully isolated from the brain of (Supplementary figure 3). The Nile tilapia NPY that was previously cloned by our laboratory (Wang et al, 2011) was renamed NPYa according to our phylogenetic and chromosomal location analysis. The mature peptides of Nile tilapia NPYa and NPYb had three and eight residues that differed from the proposed ancestral NPY, respectively, and showed 81 % similarity with each other (Fig.…”

Section: Identification and Analysis Of Nile Tilapia Npy Family Peptidesmentioning

confidence: 99%

Duplication of neuropeptide Y and peptide YY in Nile tilapia Oreochromis niloticus and their roles in food intake regulation

et al. 2017

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In vertebrates, the neuropeptide Y (NPY) family peptides have been recognized as key players in food intake regulation. NPY centrally promotes feeding, while peptide YY (PYY) and pancreatic polypeptide (PP) mediate satiety. The teleost tetraploidization is well-known to generate duplicates of both NPY and PYY; however, the functional diversification between the duplicate genes, especially in the regulation of food intake, remains unknown. In this study, we identified the two duplicates of NPY and PYY in Nile tilapia (Oreochromis niloticus). Both NPYa and NPYb were primarily expressed in the central nervous system (CNS), but the mRNA levels of NPYb were markedly lower than those of NPYa. Hypothalamic mRNA expression of NPYa, but not NPYb, decreased after feeding and increased after 7-days of fasting. However, both NPYa and NPYb caused a significant increase in food intake after an intracranial injection of 50ng/g body weight dose. PYYb, one of the duplicates of PYY, had an extremely high expression in the foregut and midgut, whereas another form of duplicate PYYa showed only moderate expression in the CNS. Both hypothalamic PYYa and foregut PYYb mRNA expression increased after feeding and decreased after 7-days of fasting. Furthermore, the intracranial injection of PYYb decreased food intake, but PYYa had no significant effect. Our results suggested that although the mature peptides of NPYa and NPYb can both stimulate food intake, NPYa is the main endogenous functional NPY for feeding regulation. A functional division has been identified in the duplicates of PYY, which deems PYYb as a gut-derived anorexigenic peptide and PYYa as a CNS-specific PYY in Nile tilapia.

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Section: Identification and Analysis Of Nile Tilapia Npy Family Peptidesmentioning

confidence: 99%

Duplication of neuropeptide Y and peptide YY in Nile tilapia Oreochromis niloticus and their roles in food intake regulation

et al. 2017

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Genes involved in fatty acid metabolism: Molecular characterization and hypothalamic mRNA response to energy status and neuropeptide Y treatment in the orange-spotted grouper Epinephelus coioides

Tang

Sun

Yan

et al. 2013

Molecular and Cellular Endocrinology

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Neuropeptide B in Nile tilapia Oreochromis niloticus: Molecular cloning and its effects on the regulation of food intake and mRNA expression of growth hormone and prolactin

Yang

Sun

2014

General and Comparative Endocrinology

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Expression of Neuropeptide Y of GIFT Tilapia (Oreochromis sp.) in Yeast Pichia Pastoris and Its Stimulatory Effects on Food Intake and Growth

Cited by 3 publications

References 38 publications

Duplication of neuropeptide Y and peptide YY in Nile tilapia Oreochromis niloticus and their roles in food intake regulation

Duplication of neuropeptide Y and peptide YY in Nile tilapia Oreochromis niloticus and their roles in food intake regulation

Genes involved in fatty acid metabolism: Molecular characterization and hypothalamic mRNA response to energy status and neuropeptide Y treatment in the orange-spotted grouper Epinephelus coioides

Neuropeptide B in Nile tilapia Oreochromis niloticus: Molecular cloning and its effects on the regulation of food intake and mRNA expression of growth hormone and prolactin

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