Expression of nestin, desmin and vimentin in intact and regenerating muscle spindles of rat hind limb skeletal muscles

Abstract

We describe the expression and distribution patterns of nestin, desmin and vimentin in intact and regenerating muscle spindles of the rat hind limb skeletal muscles. Regeneration was induced by intramuscular isotransplantation of extensor digitorum longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. The host muscles with grafts were excised after 7-, 16-, 21- and 29-day survival and immunohistochemically stained. Nestin expression in intact spindles in host muscles was restricted to Schwann cells of sensory and motor nerves. In transplanted muscles, however, nestin expression was also found in regenerating "spindle fibers", 7 and 16 days after grafting. From the 21st day onwards, the regenerated spindle fibers were devoid of nestin immunoreactivity. Desmin was detected in spindle fibers at all developmental stages in regenerating as well as in intact spindles. Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting. Our results show that the expression pattern of these intermediate filaments in regenerating spindle fibers corresponds to that found in regenerating extrafusal fibers, which supports our earlier suggestion that they resemble small-diameter extrafusal fibers.

Effects of Topical Icing on Inflammation, Angiogenesis, Revascularization, and Myofiber Regeneration in Skeletal Muscle Following Contusion Injury

“…Nestin has been detected in endothelial cells (Mokry et al, 2004; Cizkova et al, 2009b), pericytes (Birbrair et al, 2014), and blood vessels (Amoh et al, 2005) within skeletal muscle. Consistent with other research (Vaittinen et al, 2001; Cizkova et al, 2009a,b), we observed that nestin expression was greatest in regenerating muscle tissue between 3 and 7 d after injury. At 3 d after injury, there appeared to be strong nestin staining around blood vessels (Figure 12), suggesting that nestin was expressed by maturing endothelial cells (Cizkova et al, 2009b).…”

Functional genomics of the muscle response to restraint and transport in chickens1

“…Several proteins differentially expressed can have physical links with AGTAl: RDX (Luciani et al, 2002), XIRPl (Gherepanova et al, 2006), GFL2 (Ono, 2003), TRIM63 (Witt et al, 2008), ABRA (Barrientos et al, 2007), VIM (Gízková et al, 2009), PDGD6IP (Pan et al, 2006), IPP (Kim et al, 1999;VanHouten et al, 2001), and MPRIP (Riddick et al, 2008). In skeletal muscle, AGTAl is involved in contractile activity.…”

“…Furthermore, ABRA action prerequires the activation of the Rho GTPase and actin polymerization (Barrientos et al,, 2007), The protein VIM belongs to the intermediate filaments and is involved in numerous functions such as cell adhesion, migration, and signaling (for review, see Ivaska et al, 2007). This protein is present in newly differentiated muscle cells and in regenerating muscle fibers (Gízková et al, 2009). The protein PDGD6IP has several functions in cells including apoptosis, endocytic membrane trafficking, and cytoskeletal remodeling (for review see Odorizzi, 2006).…”

(−)-Epigallocatechin-3-gallate (EGCG) attenuates functional deficits and morphological alterations by diminishing apoptotic gene overexpression in skeletal muscles after sciatic nerve crush injury

“…In addition, desmin was demonstrated to be essential in muscle regeneration and present in myofibrils and myoblasts (Lawson-Smith and McGeachie 1998). Thus, as a result of injury, desmin expression is expected to increase (White et al 2002;Cízková et al 2009), and its elevated levels may have peak at 4 weeks of training and then it starts to drop slowly (Woolstenhulme et al 2006).…”