Expression of nerve growth factor and hypoxia inducible factor-1α and its correlation with angiogenesis in non-small cell lung cancer

Abstract

In order to investigate the expression of nerve growth factor (NGF) and hypoxia inducible factor-1α (HIF-1α) and its correlation with angiogenesis in non-small cell lung cancer (NSCLC), paraffin-embedded tissue blocks from 20 patients with NSCLC were examined. Twenty corresponding para-cancerous lung tissue specimens were obtained to serve as a control. The expression of NGF, HIF-1α, and vascular endothelial growth factor (VEGF) in the NSCLC tissues was detected by using immunohistochemistry. The microvascular density (MVD) was determined by CD31 staining. The results showed that the expression levels of NGF, HIF-1α and VEGF in the NSCLC tissues were remarkably higher than those in the para-cancerous lung tissues (P<0.05). There was significant difference in the MVD between the NSCLC tissues (9.19±1.43) and para-cancerous lung tissues (2.23±1.19) (P<0.05). There were positive correlations between NGF and VEGF, between HIF-1α and VEGF, and between NGF and HIF-1α in NSCLC tissues, with the spearman correlation coefficient being 0.588, 0.519 and 0.588, respectively. In NSCLC tissues, the MVD had a positive correlation with the three factors (P<0.05). Theses results suggest that NGF and HIF-1α are synergically involved in the angiogenesis of NSCLC.

Administration of CoCl2 Improves Functional Recovery in a Rat Model of Sciatic Nerve Transection Injury

“…It is well-known that neurotrophin and its receptor exert their significant roles in neurogenesis and neuroprotection. NGF, BDNF and GDNF are the main members that can stimulate the survival, regeneration and function of neurons 43 , 44 . As previous researches reported, HIF-1α and NGF are over-expressed synergically involved in the angiogenesis 43 , 45 .…”

“…NGF, BDNF and GDNF are the main members that can stimulate the survival, regeneration and function of neurons 43 , 44 . As previous researches reported, HIF-1α and NGF are over-expressed synergically involved in the angiogenesis 43 , 45 . Nakamura et al reported that NGF-induced VEGF transcription was dependent on the increased production of HIF-1α in neuroblastoma.…”

Nerve Growth Factor: A Potential Therapeutic Target for Lung Diseases

“…NGF, a critical mediator in neuro-immune mechanisms of asthma, aggravates inflammation in asthma and airway remodeling [19]. NGF boosts angiogenesis in tumor tissue, including non-small cell lung cancers (NSCLCs) [20]. Serum levels of NGF are also extremely high in COPD subjects [14].…”

“…Meanwhile, in tumor tissues, the overexpression of NGF and hypoxia-inducible factor-1α (HIF-1α) boosts angiogenesis [104,105]. A recent study has also found that compared with para-cancerous lung tissues, NGF and HIF-1α are highly expressed in NSCLC tissues [20]. The expression of NGF and HIF-1α [106] is intently correlated with the microvascular density in NSCLC.…”

The antitumor mechanisms of aerobic exercise: A review of recent preclinical studies

“… 23 It has also been found that HIF‐1α is associated with angiogenesis in non‐small‐cell lung cancer (NSCLC), and the expression of HIF‐1α and VEGF are positively correlated and significantly higher in lung cancer tissues compared with normal tissues. 25 …”