Expression of Mytilus immune genes in response to experimental challenges varied according to the site of collection

Li, Hui; Venier, Paola; Prado-Álvarez, María; Gestal, Camino; Toubiana, Mylène; Quartesan, Rosita; Borghesan, F.; Novoa, Beatriz; Figueras, António; Roch, Philippe

doi:10.1016/j.fsi.2009.12.022

Cited by 31 publications

(25 citation statements)

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“…In vivo treatment of mussels and tissue sampling Adult Mediterranean mussels, M. galloprovincialis (PalavasPrévost lagoon, France), challenged by injecting into the posterior adductor muscle 100 ll of SSW (sterile seawater) with 10 7 of the Gram-negative bacteria, V. splendidus LGP32 (Gay et al, 2004), V. anguillarum (Institut Pasteur-France ATCC 19264), or of the Gram-positive bacterium, M. luteus (Institut Pasteur-France ATCC 4698), were from (Li et al, 2010). M. galloprovincialis challenged with 2 Â 10 4 spores of filamentous fungus, F. oxysporum, were from .…”

Section: Bioinformatic Analysismentioning

confidence: 99%

“…Similarly, the temporal control of NF-jB activation in mammals is coordinated by degradation and synthesis of IjB proteins which are responsible for the strong negative regulation allowing a fast turn-off of the NF-jB response (Hoffmann et al, 2002). On the opposite, up-and down-regulation of the various AMP genes in M. galloprovincialis was reported by several laboratories using different techniques (Costa et al, 2009b;Li et al, 2010;Mitta et al, 2000a;Pallavicini et al, 2008;Venier et al, 2011) and appears somehow conflicting. Details related to the experimental challenges (e.g.…”

Section: Tablementioning

confidence: 99%

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Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Toubiana

Rosani

Giambelluca

et al. 2014

Developmental & Comparative Immunology

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a b s t r a c tBased on protein domain structure and organization deduced from mRNA contigs, 15 transcripts of the Toll signaling pathway have been identified in the bivalve, Mytilus galloprovincialis. Identical searches performed on publicly available Mytilus edulis ESTs revealed 11 transcripts, whereas searches performed in genomic and new transcriptome sequences of the Pacific oyster, Crassostrea gigas, identified 21 Toll-related transcripts. The remarkable molecular diversity of TRAF and IKK coding sequences of C. gigas, suggests that the sequence data inferred from Mytilus cDNAs may not be exhaustive. Most of the Toll pathway genes were constitutively and ubiquitously expressed in M. galloprovincialis, although at different levels, and clearly induced after in vivo injection with bacteria. Such over-transcription was more rapid and intense with Gram-negative than with Gram-positive bacteria. Injection of a fungus modulated the transcription of few Toll pathway genes, with the induction levels of TLR/MyD88 complex being always less intense. Purified LPS and b-glucans had marginal effect whereas peptidoglycans were ineffective. At the moment, we found no evidence of an IMD transcript in bivalves. In conclusion, mussels possess a complete Toll pathway which can be triggered either by Gram-positive or Gram-negative bacteria.

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Section: Bioinformatic Analysismentioning

confidence: 99%

Section: Tablementioning

confidence: 99%

Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Toubiana

Rosani

Giambelluca

et al. 2014

Developmental & Comparative Immunology

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“…The longest isotig of each isogroup, the contigs, the singletons with more than 200 bp of continuous sequence with a Phred Q > 20 (16,495) and the R. philippinarum ESTs in the NCBI database (2,050) were then considered for the annotation. The putative identities of these sequences were obtained by running BlastX and BlastN similarity searches in 48 different protein and nucleotide databases.…”

Section: Resultsmentioning

confidence: 99%

“…Although molluscs lack a specific immune system, the innate response involving circulating hemocytes and a large variety of molecular effectors seems to be an efficient defense method for responding to external aggressions by detecting the molecular signatures of an infection [7][8][9][10][11]. Hemocytes are the main line of cellular defense against invading pathogens in molluscs [12], and the immune genes expressed in bivalve hemocytes are of great interest to researchers [13][14][15][16][17][18][19]. In recent years, a serious effort has also been made to increase the number of R. philippinarum sequences in public databases.…”

Section: Introductionmentioning

confidence: 99%

Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus challenge using an immune-enriched oligo-microarray

Moreira

Milan

Balseiro

et al. 2013

Fish & Shellfish Immunology

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“…After injection, mussels were returned to seawater at 20 C. cDNAs from mussels challenged with bacteria were from Li et al [22]. Briefly, mussels from Palavas-France were challenged by one injection of 10 7 Vibrio splendidus LGP32, Vibrio anguillarum (Gram-) or Micrococcus lysodeikticus (Gramþ) into the posterior adductor muscle and returned to seawater at 20 C.…”

Section: In Vivo Challenges Rna Extraction and Reverse Transcriptionmentioning

confidence: 99%

Gene expression specificity of the mussel antifungal mytimycin (MytM)

Sonthi

Cantet

Toubiana

et al. 2012

Fish & Shellfish Immunology

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a b s t r a c tWe previously reported the nucleotide sequences and diversity of mytimycin (MytM) from the Mediterranean mussel, Mytilus galloprovincialis. Using real-time PCR (q-PCR), we observed that the MytM gene was mainly expressed in circulating hemocytes and to a less extent in the mantle. In vivo challenge with bacteria or with the yeast, Candida albicans, did not increase the expression as measured by q-PCR in hemocytes. By contrast, injection of the filamentous fungus, Fusarium oxysporum, induced a sudden and strong increase of expression at 9h p.i. (stimulation index of 25.7 AE 2.1). Optimum stimulating dose was 10 4 spores of F. oxysporum per mussel. In the same samples, AMP mytilin and myticin showed no stimulation. Consequently, we hypothesized the existence of 2 different signal transduction pathways, one activated by bacteria and yeast, the other triggered by filamentous fungi. A second challenge performed with F. oxysporum 24 h after the first challenge induced an increase of MytM gene expression (stimulation index of 3.5 AE 1.7). However, this second increase was significantly lower than the first, suggesting less efficient response rather than significant protection.

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Expression of Mytilus immune genes in response to experimental challenges varied according to the site of collection

Cited by 31 publications

References 40 publications

Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus challenge using an immune-enriched oligo-microarray

Gene expression specificity of the mussel antifungal mytimycin (MytM)

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