Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

Abstract

Background MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown. Results In this study, to examine its functions, Agrobacterium -mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T 2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.2% compared to wild-type (WT) lines. In T 2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA 1 ) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic plants were notably decreased. Conclusions Taken together, it proposed that ZmMYB59 plays a negative regulation during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

PpMYB52 negatively regulates peach bud break through the gibberellin pathway and through interactions with PpMIEL1

“…In previous studies, MYB TFs have been found to be closely related to seed germination. Specifically, AtMYB44, AtMYB96, and ZmMYB59 play a negative regulatory role in seed germination (Nguyen et al, 2012;Lee and Seo, 2015;Zhai et al, 2020). In this study, we found that the expression of PpMYB52 was maintained at a high level in the endodormancy stage, continued decrease throughout bud break, and decreased to the lowest level before bud break (Figure 2B).…”

“…In wheat, dormancy release was shown to occur earlier after the TaMyB10-A1 gene was mutated (Mares and Himi, 2021). In maize, ZmMYB59 plays a negative regulatory role in germination (Zhai et al, 2020). Studying tomato, Xu et al (2018) found that overexpression of SlMYB102 can improve the seed germination rate.…”