“…Likewise, TIMP3 turnover is reduced in ARPE19 cells overexpressing p.(Ser179Cys), p.(Glu162*) and [(p.(Ser204Cys)] TIMP3 mutants, but remains unaffected in mouse fibroblasts expressing the p.(Ser179Cys) TIMP3 mutant. Similarly, several studies on distinct TIMP3 variants in several cell lines (BHK, COS7, ARPE‐19, patient‐derived fibroblasts) have demonstrated that mutant TIMP3 maintains its MMP2/MMP9 inhibitory function (Arris et al, ; Langton et al, ; Saihan et al, ; Yeow et al, ). In contrast, overexpression of (p.(Ser179Cys) and p.(Glu162*) TIMP3 mutants in COS7, BHK, endothelial cell, mouse fibroblast and ARPE19 cells has yielded contradictory results with respect to the retention of TIMP3’s MMP inhibitory activity (Arris et al, ; Langton et al, ; Qi et al, ; Soboleva et al, ; Yeow et al, ).…”