Expression of mRNAs Encoding for α and β Integrin Subunits, MMPs, and TIMPs in Stretched Human Periodontal Ligament and Gingival Fibroblasts

Bolcato-Bellemin, Anne-Laure; Elkaïm, R.; Abehsera, A.; Fausser, Jean-Luc; Haïkel, Youssef; Tenenbaum, Henri

doi:10.1177/00220345000790091201

Cited by 108 publications

(84 citation statements)

References 23 publications

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“…They help maintain a delicate balance between physiological degradation and synthesis of the extracellular matrix. TIMPs are widely distributed in the tissues and fluids and are expressed by many cells including fibroblasts [3]. TIMPs play an important role in MMP inhibition and ECM turnover.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Interventions in Oral Submucous Fibrosis: An Experimental and Clinical Study

Singh

Mohammad

Pant³

et al. 2014

J. Maxillofac. Oral Surg.

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Introduction Oral submucous fibrosis (OSF) is a chronic debilitating disease and premalignant condition of the oral cavity and is a serious public health issue in India and many parts of the world. The treatment is still elusive and empirical because of poorly understood etiopathogenesis, which is believed to be multifactorial including areca nut chewing, ingestion of chillies, genetic and immunologic processes, nutritional deficiencies, and many others. The present investigations was focused to understand the possible therapeutic interventions of anti-OSF agents in arecoline induced experimental in vitro model of OSF and clinical application of these anti-OSF agents in the restoration of various grade of the disease. Materials and Methods The 127 subjects were selected from patients who visited the OPD of Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, K.G. Medical University, Lucknow. Further the subjects were divided in two groups on the basis of clinical examination. Group-1 subjects showed presence of fibrosis bands in the labial and/or buccal mucosa, loss of elasticity, difficulty to open the mouth and had a habit chewing arecanut in some form. Group-2 subjects had no habit of chewing areca-nut, were apparently healthy with no mucosal disorder. The samples were collected and were immediately transported to Indian Institute of Toxicology Research, Lucknow, for isolation and cultivation of primary cultures of mucosal fibroblast cells. Then isolation and cultivation of oral mucosal fibroblast, identification of non-cytotoxic doses of arecoline, real time PCR, immunocytochemistry, cytokine determination in culture cells, western blot analyses, functional activity of collagenase, lysyl oxidase enzyme activity, collagen beads assay, cyclooxygenase (COX-2) expression analysis was done. Results and Conclusions This study, shows that the reduction of phagocytic cells was strongly related to the arecoline levels in fibroblast culture when we exposed arecoline to normal oral mucosal cells (NOMC) and cells from OSF patient. An enhancement of phagocytic cells was observed following the pre exposure of cells to 1 lM dexamethasone, a glucocorticoids, In this study, histologic evidence is presented which supports the finding that COX-2 expression is upregulated in OSF specimens compared to normal oral submucosal cells. Strong immunostaining for COX-2 was detected in arecoline exposed NOMC and cells from OSF patient. Areca nut extract up-regulates prostaglandin production, cyclooxygenase-2 mRNA and protein expression of human oral keratinocytes. The number of phagocytic cells and phagocytic activity in cultured human oral fibroblasts from OSF site was lower than the fibroblasts from the normal regions of the same person.

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Section: Discussionmentioning

confidence: 99%

Therapeutic Interventions in Oral Submucous Fibrosis: An Experimental and Clinical Study

Singh

Mohammad

Pant³

et al. 2014

J. Maxillofac. Oral Surg.

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“…21 To test the effect of integrin cell surface receptors on cell shape and cytoskeletal organization and since fibronectin is one of the major periodontal ECM proteins, we decided to focus on the two major fibronectin-associated integrin subunits a5 and b1, which are preferentially expressed to a higher extent in PDL cells. [22][23][24][25][26] To determine the effects of root surface topography on integrin-related signaling networks, we have compared mPDLPs exposed to regular 2D culture dishes (2D), 3D root surface environments in vitro (3D) (i.e., cells were grown in vitro on denuded root surfaces for 3 days before replantation), and mPDLPs that were replanted in vivo for 8 weeks (in vivo) (Fig. 2i).…”

Section: 20mentioning

confidence: 99%

Apatite Microtopographies Instruct Signaling Tapestries for Progenitor-Driven New Attachment of Teeth

Dangaria

Ito

Yin

et al. 2011

Tissue Engineering Part A

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Dimension and structure of extracellular matrix surfaces have powerful influences on cell shape, adhesion, and gene expression. Here we show that natural tooth root topographies induce integrin-mediated extracellular matrix signaling cascades in tandem with cell elongation and polarization to generate physiological periodontium-like tissues. In this study we replanted surface topography instructed periodontal progenitors into rat alveolar bone sockets for 8 and 16 weeks, resulting in complete reattachment of tooth roots to the surrounding alveolar bone with a periodontal fiber apparatus closely matching physiological controls along the entire root surface. Displacement studies and biochemical analyses confirmed that progenitor-based engineered periodontal tissues were similar to control teeth and uniquely derived from preimplantation green fluorescent protein (GFP)-labeled progenitors. Together, these studies illustrate the capacity of natural extracellular surface topographies to instruct progenitor cell populations to fully regenerate complex cellular and structural morphologies of tissues once lost to disease. We suggest that our strategy could be used for the replantation of teeth lost due to trauma or as a novel approach for tooth replacement using tooth-shaped replicas.

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“…There are increasing evidences that MMPs are essential enzymes responsible for degradation of extracellular matrix in the pathological condi- 19) . Numerous studies have examined MMPs secreted from periodontal ligament fibroblast [20][21][22] and correlated the level of expression with the invasive ability of the disease 4,6) . Individual osteoblasts, like fibroblasts, are capable of both synthesizing and degrading their respective organic matrices in vivo 23) .…”

Section: ⅳ Discussionmentioning

confidence: 99%

MMP-1 and TIMP-1 production in MG-63 cells stimulated withPrevotella nigrescenslipopolysaccharide

Yang

Kim

Shon

et al. 2004

J Korean Acad Conserv Dent

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The purpose of this study is to monitor the secretion of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) produced by human osteosarcoma cell line (MG63) stimulated with Prevotella nigrescens lipopolysaccharides (LPS), and to compare the level of secretion before and after the treatment of calcium hydroxide on P. nigrescens LPS.LPS was extracted and purified from anaerobically cultured P. nigrescens. MG63 cells were stimulated by the LPS (0, 1, 10 ㎍/㎖) or LPS (10 ㎍/㎖) pretreated with 12.5 ㎎/㎖ of Ca(OH)2 for 3 days.Total RNA was isolated from the cell, and real-time quantitative polymerase chain reaction (PCR) was performed for quantification of MMP-1 and TIMP-1.The results were as follows. 1. MMP-1 mRNA expression at 48 hr was highly increased by stimulation with P. nigrescens LPS. The increase was dose-dependent. 2. When stimulated with 1 ㎍/㎖ of LPS, TIMP-1 mRNA expression was highly increased at 24 hr and 48 hr. However, TIMP-1 expression was suppressed at higher concentration (10 ㎍/㎖). 3. When P. nigrescens LPS was pretreated with Ca(OH)2, MMP-1 and TIMP-1 gene expression was downregulated. The results of this study suggest that transcriptional regulation of MMP-1 and TIMP-1 by P. nigrescens LPS could be one of the important mechanisms in bone resorption of periapical inflammation. The result of calcium hydroxide on MMP-1 and TIMP-1 gene expression suppression shows that calcium hydroxide detoxified bacterial LPS and thus should be used the medication of choice for intracanal dressings in root canal infected with black-pigmented bacteria. [J Kor Acad Cons Dent 29(5):470-478, 2004]

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Expression of mRNAs Encoding for α and β Integrin Subunits, MMPs, and TIMPs in Stretched Human Periodontal Ligament and Gingival Fibroblasts

Cited by 108 publications

References 23 publications

Therapeutic Interventions in Oral Submucous Fibrosis: An Experimental and Clinical Study

Therapeutic Interventions in Oral Submucous Fibrosis: An Experimental and Clinical Study

Apatite Microtopographies Instruct Signaling Tapestries for Progenitor-Driven New Attachment of Teeth

MMP-1 and TIMP-1 production in MG-63 cells stimulated withPrevotella nigrescenslipopolysaccharide

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