Expression of molecular markers associated to defense signaling pathways and strigolactone biosynthesis during the early interaction tomato-Phelipanche ramosa

Torres-Vera, Rocío; García, Juan Manuel Vicent; Pozo, Marı́a J.; López‐Ráez, Juan Antonio

doi:10.1016/j.pmpp.2016.05.007

Cited by 25 publications

(37 citation statements)

References 65 publications

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“…Several reports have shown that genes involved in the jasmonic acid (JA) and/or ethylene pathways are induced in the roots of Arabidopsis ( Arabidopsis thaliana ), medicago ( Medicago truncatula ), lotus ( Lotus japonicus ) and tomato upon infection by Orobanche and Phelipanche spp. (Dos Santos et al, 2003a; Die et al, 2007; Dita et al, 2009; Hiraoka et al, 2009; Torres-Vera et al, 2016). In tomato and sunflower ( Helianthus annuus ), an infection with these parasites induced the expression of genes involved in the salicylic acid (SA) pathway (Letousey et al, 2007; Torres-Vera et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…(Dos Santos et al, 2003a; Die et al, 2007; Dita et al, 2009; Hiraoka et al, 2009; Torres-Vera et al, 2016). In tomato and sunflower ( Helianthus annuus ), an infection with these parasites induced the expression of genes involved in the salicylic acid (SA) pathway (Letousey et al, 2007; Torres-Vera et al, 2016). In sunflower, a higher expression of these genes was found to be correlated with a more resistant phenotype (Letousey et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Also abscisic acid (ABA) seems to be involved in the host–parasite interaction, as expression of ABA biosynthetic as well as ABA-responsive genes was induced in tomato upon P. ramosa infection (Torres-Vera et al, 2016). Proteomics showed that an abundance of ABA-responsive proteins is only detected in root extracts of an Orobanche crenata -resistant pea ( Pisum sativum ) cultivar (Angeles Castillejo et al, 2004), suggesting that ABA signaling is important for the plant’s defense against broomrape.…”

Section: Introductionmentioning

confidence: 99%

“…Previously, it has been suggested that SLs and ABA influence each other’s levels, and it was shown that a SL-deficient tomato line ( SlCCD8 RNAi) had reduced levels of ABA when compared with its wild type (López-Ráez et al, 2010; Torres-Vera et al, 2016). Biosynthesis of the SLs is partially elucidated and was shown to be catalyzed by a number of enzymes, including DWARF 27 (D27), CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7)/MORE AXILLARY GROWTH 3 (MAX3), CAROTENOID CLEAVAGE DIOXYGENASE 8 (CCD8)/MORE AXILLARY GROWTH 4 (MAX4), MORE AXILLARY GROWTH 1 (MAX1) and the recently identified LATERAL BRANCHING OXIDOREDUCTASE (LBO) (Booker et al, 2005; Lin et al, 2009; Alder et al, 2012; Kohlen et al, 2012; Abe et al, 2014; Zhang et al, 2014; Brewer et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, SLs have also been reported to be involved in plant defense and stress responses (Bu et al, 2014; Ha et al, 2014; Torres-Vera et al, 2014; Liu J. et al, 2015). In addition, increased expression of SL biosynthetic genes SlD27 and SlCCD8 was observed in tomato roots after P. ramosa infection, suggesting activation of the SL biosynthetic pathway during the host–parasite interaction (Torres-Vera et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

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The Role of Endogenous Strigolactones and Their Interaction with ABA during the Infection Process of the Parasitic Weed Phelipanche ramosa in Tomato Plants

et al. 2017

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The root parasitic plant species Phelipanche ramosa, branched broomrape, causes severe damage to economically important crops such as tomato. Its seed germination is triggered by host-derived signals upon which it invades the host root. In tomato, strigolactones (SLs) are the main germination stimulants for P. ramosa. Therefore, the development of low SL-producing lines may be an approach to combat the parasitic weed problem. However, since SLs are also a plant hormone controlling many aspects of plant development, SL deficiency may also have an effect on post-germination stages of the infection process, during the parasite-host interaction. In this study, we show that SL-deficient tomato plants (Solanum lycopersicum; SlCCD8 RNAi lines), infected with pre-germinated P. ramosa seeds, display an increased infection level and faster development of the parasite, which suggests a positive role for SLs in the host defense against parasitic plant invasion. Furthermore, we show that SL-deficient tomato plants lose their characteristic SL-deficient phenotype during an infection with P. ramosa through a reduction in the number of internodes and the number and length of secondary branches. Infection with P. ramosa resulted in increased levels of abscisic acid (ABA) in the leaves and roots of both wild type and SL-deficient lines. Upon parasite infection, the level of the conjugate ABA-glucose ester (ABA-GE) also increased in leaves of both wild type and SL-deficient lines and in roots of one SL-deficient line. The uninfected SL-deficient lines had a higher leaf ABA-GE level than the wild type. Despite the high levels of ABA, stomatal aperture and water loss rate were not affected by parasite infection in the SL-deficient line, while in wild type tomato stomatal aperture and water loss increased upon infection. Future studies are needed to further underpin the role that SLs play in the interaction of hosts with parasitic plants and which other plant hormones interact with the SLs during this process.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Role of Endogenous Strigolactones and Their Interaction with ABA during the Infection Process of the Parasitic Weed Phelipanche ramosa in Tomato Plants

et al. 2017

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Hormonal Regulation of Heavy Metal Toxicity and Tolerance in Crop Plants

Kido,

de Andrade Luz,

da Silva

et al. 2023

Heavy Metal Toxicity and Tolerance in Plants

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Accumulation of azafrin in the root apoplast of the medicinal plant Escobedia grandiflora might play a role in parasitism

Medina

Santos

Nodari

et al. 2023

Plants People Planet

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Societal Impact Statement Escobedia grandiflora is a medicinal hemiparasite that occurs naturally in non‐forested communities in Central and South America. Parasitic plants accumulate high levels of a water‐soluble orange pigment in roots that was once among the most important food dyes in the Andean region. We conclusively address the chemical identification of this pigment and provide molecular and cellular insights on its biosynthesis and possible function. Summary The herbaceous hemiparasite Escobedia grandiflora (Orobanchaceae) is used in traditional medicine in the Andean region. Escobedia roots accumulate high levels of an orange pigment with a significant relevance as a cooking dye that exhibits antioxidant and cardioprotective properties. Here, we aimed to confirm the chemical identity of the pigment and investigate its biosynthesis and function in Escobedia roots. We combined metabolic and cytological analyses with de novo transcriptome assembly, gene expression studies, and phylogenetic analyses. The pigment was conclusively shown to be azafrin, an apocarotenoid likely derived from the cleavage of β‐carotene. RNA‐seq supported by multispecies comparative transcriptome analysis and qRT‐PCR allowed to propose candidate genes for the production of azafrin in Escobedia roots. We also showed that azafrin is delivered to the root apoplast and that it accumulates in the area where the Escobedia haustorium contacts the host's root. Our data suggest that azafrin production might rely on a carotenoid cleavage dioxygenase (CCD) different from CCD7 and that this apocarotenoid might function in the parasitism process. Together, our work represents an unprecedented step forward in our understanding of the Escobedia parasitization system.

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Expression of molecular markers associated to defense signaling pathways and strigolactone biosynthesis during the early interaction tomato-Phelipanche ramosa

Cited by 25 publications

References 65 publications

The Role of Endogenous Strigolactones and Their Interaction with ABA during the Infection Process of the Parasitic Weed Phelipanche ramosa in Tomato Plants

The Role of Endogenous Strigolactones and Their Interaction with ABA during the Infection Process of the Parasitic Weed Phelipanche ramosa in Tomato Plants

Hormonal Regulation of Heavy Metal Toxicity and Tolerance in Crop Plants

Accumulation of azafrin in the root apoplast of the medicinal plant Escobedia grandiflora might play a role in parasitism

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