Expression of miRNA-26a in platelets is associated with clopidogrel resistance following coronary stenting

Chen, Shuxia; Qi, Xiaoyong; Chen, Hua; Li, Mingquan; Gu, Jianxin; Liu, Chunxia; Xue, Hua; Wang, Lili; Geng, Yue; Peng, Qi

doi:10.3892/etm.2016.3278

Cited by 19 publications

(26 citation statements)

References 34 publications

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“…Additionally, plasma levels of miR-223 are decreased in patients with essential thrombocytopenia [ 10 ]. miR-23a is highly abundant in platelets [ 11 ] but correlates neither with the degree of platelet activation nor with clopidogrel resistance [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures

Mußbacher¹,

Krammer²,

Heber

et al. 2020

Cells

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Blood-derived microRNA signatures have emerged as powerful biomarkers for predicting and diagnosing cardiovascular disease, cancer, and metabolic disorders. Platelets and platelet-derived microvesicles are a major source of microRNAs. We have previously shown that the inappropriate anticoagulation and storage of blood samples causes substantial platelet activation that is associated with the release of platelet-stored molecules into the plasma. However, it is currently unclear if circulating microRNA levels are affected by artificial platelet activation due to suboptimal plasma preparation. To address this issue, we used a standardized RT-qPCR test for 12 microRNAs (thrombomiR®, TAmiRNA GmbH, Vienna, Austria) that have been associated with cardiovascular and thrombotic diseases and were detected in platelets and/other hematopoietic cells. Blood was prevented from coagulating with citrate–theophylline–adenosine–dipyridamole (CTAD), sodium citrate, or ethylenediaminetetraacetic acid (EDTA) and stored for different time periods either at room temperature or at 4 °C prior to plasma preparation and the subsequent quantification of microRNAs. We found that five microRNAs (miR-191-5p, miR-320a, miR-21-5p, miR-23a-3p, and miR-451a) were significantly increased in the EDTA plasma. Moreover, we observed a time-dependent increase in plasma microRNAs that was most pronounced in the EDTA blood stored at room temperature for 24 h. Furthermore, significant correlations between microRNA levels and plasma concentrations of platelet-stored molecules pointed towards in vitro platelet activation. Therefore, we strongly recommend to (i) use CTAD as an anticoagulant, (ii) process blood samples as quickly as possible, and (iii) store blood samples at 4 °C whenever immediate plasma preparation is not feasible to generate reliable data on blood-derived microRNA signatures.

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Section: Introductionmentioning

confidence: 99%

Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures

Mußbacher¹,

Krammer²,

Heber

et al. 2020

Cells

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“…Последовательный количественный ПЦР-анализ показал, что экспрессия микро-РНК-26а в тромбоцитах была значительно повышена у пациентов со слабым ответом на терапию клопидогрелом. Эти результаты дают основание полагать, что экспрессия микро-РНК-26а в тромбоцитах ассоциирована с высокой остаточной реактивностью тромбоцитов и микро-РНК-26а могут принимать участие в регуляции активности тромбоцитов на фоне применения клопидогрела [8].…”

Section: ìèêðî-ðíê è èíãèáèòîðû P2y12-ðåöåïòîðîâunclassified

Micro-RNA as a new biomarker of activity of the cytochrome system P-450: significance for predicting the antiplatelet action of P2Y12 receptor inhibitors

Rytkin

Игоревич²,

Мирзаев

et al. 2019

Terapevticheskii arkhiv

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“…miR-223 levels are significantly down regulated in low responders to anti-platelet therapy [45,74]. Furthermore, expression of platelet miR-26a has been linked with clopidogrel resistance during coronary stenting [75]. This theory is strengthened by research demonstrating how the switch from dual anti-platelet treatment with clopidogrel to ticagrelor is linked with significant changes in the level of platelet-specific circulating miRNAs, namely miR-223, miR-126 and miR-150 and miR-96 [76].…”

Section: Platelets Lifestyle and Mirna In The Aetiology Of Cvdmentioning

confidence: 99%

Platelets: Functional Biomarkers of Epigenetic Drift

Twomey¹,

Wallace²,

Mangone³

et al. 2019

Homeostasis - An Integrated Vision

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Cardiovascular disease (CVD) risk factors can be classed as modifiable or non-modifiable. Physical inactivity and obesity represent major behavioural risk factors for the initiation, development and progression of CVD. Platelet dysfunction is pivotal to the aetiology of CVD, a chronic vascular inflammatory condition, which is characterised by a lag time between onset and clinical manifestation. This indicates the role of epigenetic drift, defined by stochastic patterns of gene expression not dependent on dynamic changes in coding DNA. The epigenome, a collection of chemical marks on DNA and histones, is established during embryogenesis and modified by age and lifestyle. Biogenesis and effector function of non-coding RNA, such as microRNA, play a regulatory role in gene expression and thus the epigenetic mechanism. In this chapter, we will focus on the effect of the modifiable risk factors of physical activity/inactivity and overweight/obesity on platelet function, via epigenetic changes in both megakaryocytopoiesis and thrombopoiesis. We will also discuss the role of acute exercise on platelet function and the impact of cardiorespiratory fitness (CRF) on platelet responses to acute exercise. This chapter will highlight the potential role of platelets as circulating functional biomarkers of epigenetic drift to implement, optimise and monitor CVD preventive management strategies.

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Expression of miRNA-26a in platelets is associated with clopidogrel resistance following coronary stenting

Abstract: Abstract. The present study aimed to evaluate the association between platelet microRNA (miRNA)-26a expression and clopidogrel resistance in patients who underwent coronary stenting. Between

Cited by 19 publications

References 34 publications

Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures

Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures

Micro-RNA as a new biomarker of activity of the cytochrome system P-450: significance for predicting the antiplatelet action of P2Y12 receptor inhibitors

Platelets: Functional Biomarkers of Epigenetic Drift

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