Expression of miR-148b-3p is correlated with overexpression of biomarkers in prostate cancer

Abstract

Prostate cancer (PCa) is one of the leading causes of death among men. Genes such as PCA3, PSA, and Fra-1 are suggested to serve as potential tools for the detection of PCa, as they are deregulated during this pathology. A similar event occurs with small non-coding RNAs, called miRNAs, specifically miR-195-5p, miR-133a-3p, and miR-148b-3p, which were analyzed in a Chinese population and suggested to be possible candidates for PCa diagnosis. We evaluated the expression levels of three miRNAs and three genes in tissue samples of PCa and benign prostate disease, such as benign prostatic hyperplasia, or prostatitis, in order to determine their potential as candidates for PCa detection. Our results showed a statistically significant overexpression of 279-fold increase in PSA levels and a 1,012-fold increase in PCA3 levels in PCa patients compared to benign prostate disease patients (p = 0.001 and p = 0.002, respectively). We observed a positive correlation between the expression of miR-148b-3p and the expression of PSA and PCA3 genes, two established biomarkers in PCa. The expression of miR-148b-3p was not related to clinical characteristics, such as age and weight, as observed for the other miRNAs analyzed, suggesting its potential as a biomarker for detection of this pathology.

Overexpression of DDIT4 and TPTEP1 are associated with metastasis and advanced stages in colorectal cancer patients: a study utilizing bioinformatics prediction and experimental validation

“…It was described in a meta-analysis report that dysregulation of miRNA-181d family membrane can be used as prognostic marker in different cancers [ 34 ]. Also, dysregulation of miRNA-148b-3p expression was reported in numerous cancers including breast [ 35 ], thyroid [ 36 ], prostate [ 37 ], colorectal [ 38 ] and gastric cancer [ 39 ]. Until now, dysregulated expression of TPTEP1 has been reported mainly in patients with human lung [ 40 ] and liver cancer [ 41 ].…”

WITHDRAWN: LncRNA HOXA11-AS Aggravates The Keloid Formation By Targeting miR-148b-3p/IGFBP5 Axis

“…In our present work, bioinformatics analysis was applied and predicted miR148b-3p is a critical player in HOXA11-AS-IGFBP5 regulation platform in keloid. Deregulation of miR-148b-3p has been found in cancers, arthritis, ischemic stroke, heart failure [16][17][18][19][20]. However, the biofunction of miR148b-3p has not been previously reported.…”

Network Analysis Integrating microRNA Expression Profiling with MRI Biomarkers and Clinical Data for Prostate Cancer Early Detection: A Proof of Concept Study

“…As displayed in the miRNA-target gene interaction maps, these differentially expressed MicroRNAs (DEmiRNAs) are also drivers of imaging biomarkers, further endorsing the value of mpMRI as a triage test. Within this group of selected DEMs, evidence in literature already exists on the possible role of let-7b-5p as biomarker specific of aggressive cancers [37,38], miR-195-5p [39,40], miR-148b-3p [38,41], miR-518c-3p [42,43] as biomarkers specific of csPCa.…”