Expression of miR-1-3p, miR-16-5p and miR-122-5p as Possible Risk Factors of Secondary Cardiovascular Events

Abstract

Ischemic event in one arterial territory increases the risk of a subsequent ischemic event. Circulating microRNAs (miRs) emerge as a potential clinical tool to assess risk of subsequent atherothrombotic events such as cardiovascular death (CVD), myocardial infarction (MI) and ischemic stroke (IS). In this prospective study, we searched for athero-specific miRs related to cardiovascular event risk in patients with symptomatic coronary, carotid lesion, or both territories involvements. The choice of particular miRs was based on database research (Pub-Med, Bethesda, MD, USA) taking into consideration the relationship with development of atherosclerosis and potential prognostic value. Levels of circulating miRs (miR-1-3p, miR-16-5p, miR-34a-5p, mir-122-5p, miR-124-3p, miR-133a-3p, miR-133b, miR-134-5p, miR-208b-3p, miR-375 and miR-499-5p) were compared in 142 patients with an acute ischemic event resulting from carotid and/or coronary artery stenosis, who underwent revascularization for symptomatic lesion. A 6-year prospective evaluation of CVD/MI/IS risk was performed. Patients with two-territory as compared to single-territory involvement differed in levels of miR-1-3p (p = 0.016), miR-16-5p (p < 0.001), miR-34a-5p (p = 0.018), miR-122-5p (p = 0.007), miR-124-3p (p < 0.001) and miR-499-5p (p < 0.001). During follow-up, 62 (43.7%) episodes of CVD/MI/IS occurred. In multivariate Cox analysis, miR-122-5p (HR = 1.0006, 95%CI = 1.0001–1.0011) and peripheral artery disease (PAD) (HR = 2.16, 95%CI = 1.26–3.70) were associated with CVD/MI/IS risk; miR-1-3p (HR = 2.73, 95%CI = 1.22–6.12) and PAD (HR = 3.47, 95%CI = 1.88–6.41) with CVD; miR-122-5p (HR = 1.0001, 95%CI = 1.000–1.0002) and creatinine level (HR = 1.02, 95%CI = 1.01–1.04) with IS, and miR-16-5p (HR = 1.0004, 95%CI = 1.0001–1.0008) with MI. Expression of miR-1-3p, miR-16-5p and miR-122-5p during incident ischemia may be possible risk factors of secondary cardiovascular event(s).

Alteration of circulating platelet-related and diabetes-related microRNAs in individuals with type 2 diabetes mellitus: a stepwise hypoglycaemic clamp study

“…Based on our results, one could speculate that an increased expression of the anti-inflammatory miR-16 in response to acute hypoglycaemia might provide some protection against the harmful effects of subsequent hypoglycaemia as it is negatively correlated with inflammatory biomarkers. It should be noted that upregulation of miR-16 expression was also described in patients with acute myocardial infarction [ 49 ]. It was also found that miR-16 along with miR-15a correlate with adverse events at 12-month follow-up in T2DM patients who have undergone percutaneous intervention to correct critical limb ischemia [ 50 ].…”

Inflammatory and Prothrombotic Biomarkers, DNA Polymorphisms, MicroRNAs and Personalized Medicine for Patients with Peripheral Arterial Disease

“…Vegter et al found that several miRNAs, related to PAD, could predict future CV complications in patients with heart failure [ 128 ]. Badacz et al identified miRNA-1-3p, miRNA-16-5p and miRNA-122-5p as possible risk factors for secondary ischemic events in patients with carotid and/or coronary artery disease [ 129 ]. In patients with PAD following femoral artery bypass surgery, plasma levels of miRNA-142, miRNA-223, miRNA-155, and miRNA-92a predicted CV events [ 130 ].…”

MicroRNA-124: A Key Player in Microglia-Mediated Inflammation in Neurological Diseases

“…Cerebrovascular diseases can lead to death or long-term disability and is a worldwide health concern. Plasma miR-124-3p levels were found to be elevated in patients with acute ischemic stroke (AIS) ( Badacz et al, 2021 ), and the miR-124-3p level was positively correlated with the poor prognosis of these patients based on the modified Rankin score ( Rainer et al, 2016 ). Higher plasma miR-124-3p levels were suggested to be associated with unfavorable outcomes in patients with AIS undergoing thrombolysis, and miR-124-3p is closely related with the severity of stroke ( He et al, 2019 ).…”