Expression of microRNAs in tumors of the central nervous system in pediatric patients in México

Eguía-Aguilar, Pilar; Gutiérrez-Castillo, Lisette; Pérezpeña-Díazconti, Mario; García-Chéquer, Jeanette; García-Quintana, Jorge; León, Fernando Chico-Ponce de; Gordillo-Domínguez, Luis Felipe; Torres-García, Samuel; Arenas-Huertero, Francisco

doi:10.1007/s00381-017-3569-9

Cited by 4 publications

(3 citation statements)

References 54 publications

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“…Previous studies have shown downregulation of miRNA-770-5p in tumors associated with the brain such as gliomas (19) and medulloblastomas (MB) (20). Even amongst different types of brain cancers, the expression levels of hsa-miR-770-5p was significantly lower in MB as compared to astrocytomas (21). Our observations further support these studies by demonstrating down-regulation of hsa-miR-770-5p in brain tumors.…”

Section: Discussionmentioning

confidence: 96%

Analysis of microarray data for identification of key microRNA signatures in glioblastoma multiforme

et al. 2019

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Glioblastoma multiforme (GBM) is one of the most malignant types of glioma known for its reduced survival rate and rapid relapse. Previous studies have shown that the expression patterns of different microRNAs (miRNA/miR) play a crucial role in the development and progression of GBM. In order to identify potential miRNA signatures of GBM for prognostic and therapeutic purposes, we downloaded and analyzed two expression data sets from Gene Expression Omnibus profiling miRNA patterns of GBM compared with normal brain tissues. Validated targets of the deregulated miRNAs were identified using MirTarBase, and were mapped to Search Tool for the Retrieval of Interacting Genes/Proteins, Database for Annotation, Visualization and Integrated Discovery and Kyoto Encyclopedia of Genes and Genomes databases in order to construct interaction networks and identify enriched pathways of target genes. A total of 6 miRNAs were found to be deregulated in both expression datasets studied. Pathway analysis demonstrated that most of the target genes were enriched in signaling cascades connected to cancer development, such as ‘Pathways in cancer’, ‘Focal adhesion’ and ‘PI3K-Akt signaling pathway’. Of the five target genes that were enriched in the glioblastoma pathway, in the WikiPathway database, both HRas proto-oncogene, GTPase and MET proto-oncogene, receptor tyrosine kinase target genes of hsa-miR-139-5p, were found to be significantly associated with patient survival. The present study may thus form the basis for further exploration of hsa-miR-139-5p, not only as a therapeutic agent, but also as a diagnostic biomarker for GBM as well as a predictive marker for patient survival.

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Section: Discussionmentioning

confidence: 96%

Analysis of microarray data for identification of key microRNA signatures in glioblastoma multiforme

et al. 2019

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“…With 200 ng of the purified RNA, the cDNA synthesis was performed using the First Strand cDNA Synthesis Kit (Thermo Scientific). The primers for HIVEP2 and GRIN2B were designed and evaluated by RT-qPCR ( Supplementary Table S1 ) and performed qPCR with the Maxima SYBR Green qPCR Master mix kit (Thermo Scientific) according to previous reports [ 63 ]. The R-based pcr package was used for qPCR data analysis [ 64 ].…”

Section: Methodsmentioning

confidence: 99%

The Transcriptomic Landscape of Pediatric Astrocytoma

Hernández‐Hernández

López-Santaella

Torres-Caballero

et al. 2022

IJMS

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Central nervous system tumors are the most common solid neoplasia during childhood and represent one of the leading causes of cancer-related mortality. Tumors arising from astrocytic cells (astrocytomas) are the most frequently diagnosed, and according to their histological and pathological characteristics, they are classified into four categories. However, an additional layer of molecular classification considering the DNA sequence of the tumorigenesis-associated genes IDH1/2 and H3F3A has recently been incorporated into the classification guidelines. Although mutations in H3F3A are found exclusively in a subtype of grade IV pediatric astrocytoma, mutations in IDH1/2 genes are very rare in children under 14 years of age. The transcriptomic profiles of astrocytoma in adults and children have been extensively studied. However, there is scarce information on these profiles in pediatric populations considering the status of tumorigenesis-associated genes. Therefore, here we report the transcriptomic landscape of the four grades of pediatric astrocytoma by RNA sequencing. We found several well-documented biological functions associated with the misregulated genes in the four grades of astrocytoma, as well as additional biological pathways. Among the four grades of astrocytoma, we found shared misregulated genes that could have implications in tumorigenesis. Finally, we identified a transcriptional signature for almost all grades of astrocytoma that could be used as a transcription-based identification method.

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“…It has been found that miR-210 can facilitate proliferation and inhibit the apoptosis of vascular smooth muscle cells, thereby promoting cell growth [ 9 ]. The abnormal miR-100-5p has been reported to participate in cerebral disease including glioblastoma [ 10 ], neurodegeneration [ 11 ], and tumors in the central nervous system [ 12 ]. Moreover, decreased miR-100 was reported to regulate ischemia in stroke and blood vessels [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

miR-100-5p activation of the autophagy response through inhibiting the mTOR pathway and suppression of cerebral infarction progression in mice

Cao,

Zhang,

Chen

et al. 2023

Aging

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In recent years, the association between microRNAs (miRNAs) and autophagy in cerebral infarction (CI) has attracted increasingly more attention. The mammalian target of the rapamycin (mTOR) pathway is a key protein regulating the autophagy response. miR-100-5p can bind to the mTOR protein, but its role in CI remains unclear yet. This experiment aims to clarify the role of miR-100-5p in CI. Bioinformatics analysis was performed to screen differentiated expressed functional genes between CI tissue and normal tissue specimens. In vivo experiments: the mouse model of CI was established by middle cerebral artery occlusion (MCAO) methods, After being treated with miR-100-5p-overexpressing lentivirus, the amount of terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL)-positive fluorescence and the fluorescent expression level of mTOR protein were significantly inhibited in the CI region. Western blotting analysis showed that miR-100-5p inhibited the protein expression level of phosphorylated mTOR and total mTOR and enhanced the expression of autophagy-related proteins Beclin, microtubule-associated protein light chain 3II (LC-3II), and autophagy-related gene 7 (ATG-7). For in vitro experiment, after the BV-2 cells were successfully infected with the control lentivirus and miR-100-5p-overexpression lentivirus, they were stimulated with 1% hypoxia and low-glucose medium in a tri-gas incubator for 24 h. It was found that miR-100-5p could significantly lower the protein expression level of phosphorylated mTOR and total mTOR, and increase the expression of the Beclin, LC-3II, ATG-7 autophagy related proteins. miR-100-5p promotes the autophagy response through binding to mTOR protein, thereby inhibiting apoptosis and delaying the progression of CI.

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Expression of microRNAs in tumors of the central nervous system in pediatric patients in México

Abstract: These microRNAs may represent potential markers in these tumors.

Cited by 4 publications

References 54 publications

Analysis of microarray data for identification of key microRNA signatures in glioblastoma multiforme

Analysis of microarray data for identification of key microRNA signatures in glioblastoma multiforme

The Transcriptomic Landscape of Pediatric Astrocytoma

miR-100-5p activation of the autophagy response through inhibiting the mTOR pathway and suppression of cerebral infarction progression in mice

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