Expression of microRNAs in the ascites of patients with peritoneal carcinomatosis and peritonitis

Schindler, Philipp; Kupčinskas, Juozas; Juzėnas, Simonas; Skiecevičienė, Jurgita; Šaltenienė, Violeta; Schulz, Christian; Weigt, Jochen; Malfertheiner, Peter; Link, Alexander

doi:10.1002/cncy.21966

Cited by 17 publications

(24 citation statements)

References 36 publications

(84 reference statements)

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“…Subsequent steps were as described above. The miRNA extraction from ascites is similar to serum and is described in detail previously . In total nine samples were used to evaluate the detectability of xenomiR miR‐168 in ascites.…”

Section: Methodsmentioning

confidence: 99%

“…Deregulation of miRNAs is an early epigenetic event and therefore potentially reversible . Certain miRNAs, for instance miR‐21, are frequently deregulated in GI tumors and are suggested as potential tumor biomarkers . We and others have shown that miRNAs can be easily detected in feces and differential expression of miRNAs may be useful for diagnosis of CRC and inflammatory bowel diseases …”

Section: Introductionmentioning

confidence: 99%

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Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

Link

Thon

Schanze

et al. 2018

Molecular Nutrition Food Res

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Scope Diet is amongst the most crucial factors contributing to the multistep process of carcinogenesis. The role of exogenous microRNAs (miRNAs) is still debatable. In this proof‐of‐principle work, the presence of miRNAs in a variety of foods, its stability to processing, and detectability in GI mucosa and feces are studied and the effect of short‐term diet on human‐ or plant‐derived miRNAs in feces and blood is examined. Methods and results Animal and plant miRNAs are detected in all foods irrespective of processing. Animal‐derived foods showed the highest miRNA level and the lowest is found in cheese and milk. The impact of the short‐term vegetarian or meat‐rich diet on blood and feces miRNA is evaluated in healthy subjects using qPCR and Affymetrix profiling. Diet is not associated with changes in ultraconserved miRNAs. However, a vegetarian diet is associated with an increase of miR‐168 in feces but not in blood. Overall, plant miR‐168 is detectable in normal GI mucosa and in colorectal cancer. Conclusions Food provides a great source of miRNAs and diet may be associated with changes in xenomiRs. Plant‐derived miR‐168 is ubiquitously present in feces, normal mucosa, and cancer. Further studies are needed to evaluate the functional interaction between diet‐derived miRNAs and GI tract.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

Link

Thon

Schanze

et al. 2018

Molecular Nutrition Food Res

Self Cite

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show abstract

“…Once the exosomes have been isolated, many commercially available kits can be used for RNA extraction. A recent study by Schindler et al of 3 different extraction methods has indicated that the extraction method can have an impact on the amount of RNA extracted from ascitic fluid specimens. After RNA extraction, miRNA quantification may be achieved by real‐time polymerase chain reaction systems or by next‐generation sequencing analysis.…”

Section: Technical Aspectsmentioning

confidence: 99%

“…Implants often are from ovarian malignancies and less frequently are from the gastrointestinal tract. Schindler et al compared miRNA expression profiles in patients with peritoneal carcinomatosis, spontaneous bacterial peritonitis (SBP), and portal hypertension without peritonitis and demonstrated a significant upregulation of miRNA‐21 and miRNA‐186 in patients with carcinomatosis versus the other groups. Overexpression of miRNA‐223 was found in SBP; this finding is not surprising because this miRNA is highly expressed in acute inflammatory cells.…”

Section: Peritoneal Carcinomatosismentioning

confidence: 99%

MicroRNA profiling in serous cavity specimens: Diagnostic challenges and new opportunities

Nicolè

Cappello

Cappellesso

et al. 2019

Cancer Cytopathology

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The cytomorphologic evaluation of serous cavity specimens can quickly determine the cause of an effusion as well as the presence or absence of a neoplastic process. Ancillary tests such as immunohistochemistry and fluorescence in situ hybridization are frequently used to help to definitively identify malignant cells, determine a site of origin, and distinguish between malignant and reactive mesothelial proliferations. In recent years, microRNAs (miRNAs) have been increasingly evaluated in cytologic specimens, including those from serous cavities. The examination of miRNA is attractive because of the stability of miRNA in such specimens and data suggesting that miRNA can provide prognostic and therapeutic information in addition to its role in diagnosis. Furthermore, miRNAs exist within extracellular exosomes, and this allows for their analysis in specimens that contain only rare malignant cells, degenerated cells, or obscuring components. This review discusses the technical aspects of specimen processing for miRNA analysis, recent studies of miRNA expression in malignant serous cavity specimens, and the potential importance of miRNA expression analysis for serous cavity specimens in the near future.

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“…Schindler et al prospectively analysed samples from patients with peritoneal carcinomatosis (PCA), spontaneous bacterial peritonitis (SBP), and portal hypertension (no SBP/PCA). As a result, a simple proportion score between miR-21 and miR-223 allowed to discriminate between the patients with PCA and those with SBP with an area under the curve of 0.982 (95% confidence interval, 0.943-1.022) [4]. Circulating miRNAs are also proposed as a biomarker of heart disease.…”

Section: Editorialmentioning

confidence: 99%

For Novel Disease Diagnostic Markers in Blood RNA

Takahashi¹,

Iwahashi²

2018

J Med Diagn Meth

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Expression of microRNAs in the ascites of patients with peritoneal carcinomatosis and peritonitis

Cited by 17 publications

References 36 publications

Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

MicroRNA profiling in serous cavity specimens: Diagnostic challenges and new opportunities

For Novel Disease Diagnostic Markers in Blood RNA

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