Expression of microRNAs during chondrogenesis of human adipose-derived stem cells

Abstract

In this study we have identified a group of miRNAs and their target genes, which may play important roles in regulating chondrogenic differentiation of hADSCs. Our results provide the basis for further investigation into the molecular mechanism of chondrogenesis in hADSCs and their differentiation for cartilage engineering.

MiR‐455‐3p regulates early chondrogenic differentiation via inhibiting Runx2

“…We found that miR-455-3p, miR-193b, miR-199a-3p/hsa-miR-199b-3p, miR-210, miR-381, miR-92a, miR-320c, and miR-136 were up-regulated [1]. In this work, we further studied the expression and regulation of one representative miRNA known to be increased in chondrogenesis, miR-455-3p [1,5]. We demonstrated a critical role for miR-455-3p as an activator in early chondrogenesis.…”

“…To explore the specific involvement of miRNAs in the chondrogenesis of MSCs, we previously used the miRNA microarray technique to profile miRNA expression and screen miRNAs with significant differences in expression before and after chondrogenic induction. We found that miR-455-3p, miR-193b, miR-199a-3p/hsa-miR-199b-3p, miR-210, miR-381, miR-92a, miR-320c, and miR-136 were up-regulated [1]. In this work, we further studied the expression and regulation of one representative miRNA known to be increased in chondrogenesis, miR-455-3p [1,5].…”

“…MiRNAs play an important role in differentiation and development across a wide range of organisms and tissue types, including bone and cartilage development [1,5,6,8,[24][25][26]38]. To explore the specific involvement of miRNAs in the chondrogenesis of MSCs, we previously used the miRNA microarray technique to profile miRNA expression and screen miRNAs with significant differences in expression before and after chondrogenic induction.…”

“…MiR-455-3p was first sequenced in 250 small RNA libraries from 26 different organ systems and types of cells in humans and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues [11]; it was then found in human cancer cells [12,13]. Recently, miR-455-3pwas found to be involved in chondrogenesis, and its actions against the DPP homologs in the (Smad)-2/3 pathway were suppressed by regulating TGFb signaling [1,5]. Based on our continuing investigation on the exact biological function and mechanisms of these miRNAs, we found that miR-455-3p was an interesting regulator during chondrogenesis and might have a role in osteoarthritis by potentially targeting Runt-related transcription factor 2 (Runx2) as we previously reported after computational analysis [1].…”

“…Recently, miR-455-3pwas found to be involved in chondrogenesis, and its actions against the DPP homologs in the (Smad)-2/3 pathway were suppressed by regulating TGFb signaling [1,5]. Based on our continuing investigation on the exact biological function and mechanisms of these miRNAs, we found that miR-455-3p was an interesting regulator during chondrogenesis and might have a role in osteoarthritis by potentially targeting Runt-related transcription factor 2 (Runx2) as we previously reported after computational analysis [1].…”