Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

Feng, Zhonggang; Takahashi, Rie; Nakamura, Toshio; Sato, Daisuke; Shirasawa, Nobuyuki; Nakayama, Ayako; Kurashige, Sakura; Kosawada, Tadashi; Kitajima, Tatsuo; Umezu, Mitsuo

doi:10.1007/s11626-014-9755-0

Cited by 6 publications

(10 citation statements)

References 29 publications

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“…In the aforementioned screens, the let-7 family members involved were all identified to be downregulated. Thus, in prostate cancer and osteosarcoma, 4 members of the let-7 family were downregulated (let-7a, b, e and f), whereas in pancreatic cancer and CML let-7a and let-7f were observed to be downregulated (3,(5)(6)(7). Let-7 is highly conserved across bilaterians, and is involved in developmental switches in C. elegans and mammals (11).…”

Section: Commonly Downregulated Micrornasmentioning

confidence: 98%

“…+ prostate cancer and osteosarcoma cells (3,7). Enforced expression of miR-34a in bulk or purified CD44 + prostate cancer cells was demonstrated to inhibit clonogenic expansion, tumor regeneration and metastasis (3).…”

Section: Commonly Downregulated Micrornasmentioning

confidence: 99%

“…+ subpopulation and SP of prostate cancer and osteosarcoma cells (3,7). The miR-15 gene is located on the chromosome 13q14, and was previously identified to be downregulated in chronic lymphocytic leukemia and pituitary adenomas (29,30).…”

Section: Mir-15 Mir-15 Was Identified To Be Downregulated In Screensmentioning

confidence: 99%

“…+ prostate cancer cells but upregulated in osteosarcoma cells (3,7). The location and expression of miR-133a, as well as its target genes, have been previously reviewed elsewhere (46), and therefore will not be discussed in the present review.…”

Section: Mir-133a Mir-133a Was Identified To Be Downregulated In Cd133mentioning

confidence: 99%

“…Screens investigating differential expression of miRNAs in various cancer cell populations have been previously performed. The present review will focus on the results of five screens conducted in the past recent years, which investigated prostate cancer (3), neuroblastoma (4), pancreatic cancer (5), chronic myeloid leukemia (CML) (6) and osteosarcoma cells (7). Specifically, Liu et al (3) (6) studied the differences in expression levels of 275 known human miRNAs in bone marrow stromal cells derived from patients with CML and normal human subjects, and identified 19 downregulated and 6 upregulated miRNAs.…”

Section: Introductionmentioning

confidence: 99%

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Diverse microRNAs with convergent functions regulate tumorigenesis

2015

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Abstract. MicroRNAs (miRNAs) regulate several biological processes, including tumorigenesis. In order to comprehend the roles of miRNAs in cancer, various screens were performed to investigate the changes in the expression levels of miRNAs that occur in different types of cancer. The present review focuses on the results of five recent screens, whereby a number of overlapping miRNAs were identified to be downregulated or differentially regulated, whereas no miRNAs were observed to be frequently upregulated. Furthermore, the majority of the miRNAs that were common to >1 screen were involved in signaling networks, including wingless-related integration site, receptor tyrosine kinase and transforming growth factor-β, or in cell cycle checkpoint control. The present review will discuss the aforementioned miRNAs implicated in cell cycle checkpoint control and signaling networks. IntroductionMicroRNAs (miRNAs) are small non-coding RNAs of 21-25 nucleotides in length that control gene expression via post-transcriptional regulation (1). Contrasting with the maturation process of normal coding RNAs, the miRNA genes are initially transcribed by RNA polymerase II into a primary transcript in the nucleus, where the hairpin structure is processed into precursor miRNA by a microprocessing complex that includes Drosha and DiGeorge syndrome chromosomal (or critical) region 8 (DGCR8) (1). Subsequently, the ~70 nucleotide-long precursor miRNA is exported into the cytoplasm, where it undergoes secondary processing by Dicer, and one strand of the hairpin is then incorporated into a ribonucleoprotein complex known as miRNA-induced silencing complex (1). Once matured, a single miRNA may target miscellaneous messenger RNAs (mRNAs), and an individual mRNA may be regulated by various miRNAs (1). miRNAs have significant roles during stem cell maintenance and differentiation (2). In addition, they have frequently been used as markers for certain types of cancer cells, by comparing the measured levels of known miRNAs with those present in their wild-type counterparts (2,3). Screens investigating differential expression of miRNAs in various cancer cell populations have been previously performed. The present review will focus on the results of five screens conducted in the past recent years, which investigated prostate cancer (3), neuroblastoma (4), pancreatic cancer (5), chronic myeloid leukemia (CML) (6) and osteosarcoma cells (7). Specifically, Liu et al (3) used three prostate cancer metastatic cell lines, namely LAPC9, LAPC4 and Du145 [bone cluster of differentiation (CD)44 + /CD44 -subpopulation sorting based on LAPC9, LAPC4 and Du145 cell lines; side population (SP)/non-SP sorting based on LAPC9 cell line; and CD133 + /CD133 -subpopulation sorting based on LAPC4 cell line], and compared the expression levels of miscellaneous miRNAs (with vs. without markers) in these cells. Following testing for 324 DGCR8 miRNAs by reverse transcription-quantitative polymerase chain reaction, 137 miRNAs were identified to be expressed in the three ce...

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Section: Commonly Downregulated Micrornasmentioning

confidence: 98%

Section: Commonly Downregulated Micrornasmentioning

confidence: 99%

Section: Mir-15 Mir-15 Was Identified To Be Downregulated In Screensmentioning

confidence: 99%

Section: Mir-133a Mir-133a Was Identified To Be Downregulated In Cd133mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diverse microRNAs with convergent functions regulate tumorigenesis

2015

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Unknown biological effects of l-glucose, ALA, and PUFA

et al. 2017

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Key substrates including glucose, amino acids, and fatty acids play core roles in nutrient metabolism. In this review, we describe phenomena observed when key substrates are applied to cells. We focused on three promising substrates: L-glucose derivatives, 5-aminolevulinic acid, and polyunsaturated fatty acid. Since they are assumed to give a specific reaction when they are transported into cells or metabolized in cells, they are expected to be applied in a clinical setting. We provide the latest knowledge regarding their behaviors and effects on cells.

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