Expression of Micro RNA in Paraffin Embedded Tissue of Multiple Myeloma

Choi, Woo Soon; Kwon, Gye Cheol

doi:10.15324/kjcls.2015.47.4.292

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…In a study of Westerners, miR-221 was overexpressed [16,17,19]. However, underexpression was found in previous studies [25]. This is to confirm the characteristic result of Korean people.…”

Section: Plasma Cell Concentrations Of Bone Marrow and Mirna Expressisupporting

confidence: 64%

Expression of MicroRNA-221 in Korean Patients with Multiple Myeloma

Choi

2018

Korean J Clin Lab Sci.

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Multiple myeloma (MM) is the leading cause of death among hematologic neoplasms. Recently, microRNA has been reported to be useful in the diagnosis of multiple myeloma. This study examined whether miR-221 could be used as a diagnostic marker for multiple myeloma. The study was performed on 20 patients with multiple myeloma without any other hematological diseases. MicroRNA extraction was performed using formalin-fixed paraffin-embedded (FFPE) tissues obtained from the bone marrow of patients with multiple myeloma. miR-15a, miR-16, miR-21, miR-181a, and miR-221 were selected as the microRNA target genes for multiple myeloma. The significance of microRNA was based on a fold change of ＜1.5. To quantify the fold changes, data normalized to the human gene, SNORD43, were used as the values of the patient group. Fold change values greater than 1.5 were defined as "overexpression", whereas values less than-1.5 were defined as "underexpression". Of note, 65.0% (13/20) of samples showed significant "overexpression" in the levels of miR-221 expression and plasma cells with a group of more and less than 30% in MM patients did not show any significance of plasma cell (P＜0.05). The results of other studies showing a correlation between the expression of miR-221 and MM in Caucasians were confirmed. These results suggest that miR-221 may be a useful indicator for diagnosing patients with MM. In conclusion, miR-221 is useful in the diagnosis and determining the prognosis of multiple myeloma in Koreans.

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“…In a study of Westerners, miR-221 was overexpressed [16,17,19]. However, underexpression was found in previous studies [25]. This is to confirm the characteristic result of Korean people.…”

Section: Plasma Cell Concentrations Of Bone Marrow and Mirna Expressisupporting

confidence: 64%

Expression of MicroRNA-221 in Korean Patients with Multiple Myeloma

Choi

2018

Korean J Clin Lab Sci.

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“…Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer www.kjcls.org cell differentiation, growth, and death [2,3]. Several studies have demonstrated the altered expression of specific miRNAs in different types of human neoplasia, suggesting that they play a key role in tumorigenesis [4,5].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer

Lee

2017

Korean J Clin Lab Sci.

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Brain Busan 21 사업단 MicroRNAs (miRNAs/miRs) are a group of small noncoding RNAs that modulate gene expression. Many studies, demonstrating altered expressions of specific miRNAs in diverse types of human neoplasia, suggested that they may play a key role in tumorigenesis. Recently, miRNA genes were found to be abnormally expressed in several types of cancer, including endometrial cancer. However, miR-23b and miR-203 expression in endometrial cancer has yet to be studied in Korea. As such, the purpose of this study was to analyze miR-23b and miR-203 expressions in endometrial cancer and to evaluate the relationship between miR-23b and miR-203 expressions. A retrospective study was carried out on the formalin-fixed, paraffin-embedded tissues of 42 endometrial cancer tissues using quantitative real-time PCR. In endometrial cancer tissues, miR-23b expression levels (2.70±4.45) were higher than miR-203 expression levels (−2.34±4.08). Endometrial cancer tissues showed an overexpression of miR-23b in 30 (71.4%) of the 42 endometrial cancer cases, whereas miR-203 was underexpressed in 29 (69.0%) of the 42 cases. There was a significant association between miR-23b and miR-203 expressions in endometrial cancer tissues (p=0.0005). These findings suggest that miR-23b and miR-203 expressions may be involved in endometrial carcinogenesis. More studies are needed to further define the relationship between miR-23b and miR-203 expressions and tissue-specific protein expression.

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Expression of Micro RNA in Paraffin Embedded Tissue of Multiple Myeloma

Cited by 2 publications

References 24 publications

Expression of MicroRNA-221 in Korean Patients with Multiple Myeloma

Expression of MicroRNA-221 in Korean Patients with Multiple Myeloma

Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer

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