Expression of Metalloproteinases and Type I and III Collagens during Healing of Excisional Skin Wound on the Abdomen and Back in Rats

Kananykhina, Evgeniya; Шмакова, Т. В.; Большакова, Г. Б.; Rusanov, F S; Elchaninov, Andrey; Nikitina, Maria; Lokhonina, Anastasia; Макаров, А. В.; Fatkhudinov, Timur

doi:10.1007/s10517-020-04808-7

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…TIMP1 plays a role in inhibiting MMP deposition and preventing ECM degradation. In addition, TIMP1 has been known to promote fibroblast proliferation and enhance new blood vessel formation [ 39 – 41 ]. This study showed that ADSCs-loaded β-ChNF hydrogel treatment up-regulated the expression of TIMP1.…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats

Liu

et al. 2022

J Mater Sci: Mater Med

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Because of stem cells are limited by the low efficiency of their cell homing and survival in vivo, cell delivery systems and scaffolds have attracted a great deal of attention for stem cells’ successful clinical practice. β-chitin nanofibers (β-ChNF) were prepared from squid pens in this study. Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy proved that β-ChNFs with the diameter of 5 to 10 nm were prepared. β-ChNF dispersion became gelled upon the addition of cell culture medium. Cell culture experiments showed that β-ChNFs exhibited negligible cytotoxicity towards ADSCs and L929 cells, and it was found that more exosomes were secreted by the globular ADSCs grown in the β-ChNF hydrogel. The vivo experiments of rats showed that the ADSCs-loaded β-ChNF hydrogel could directly cover the wound surface and significantly accelerate the wound healing and promote the generation of epithelization, granulation tissue and collagen. In addition, the ADSCs-loaded β-ChNF hydrogel clearly regulated the expressions of VEGFR, α-SMA, collagen I and collagen III. Finally, we showed that ADSCs-loaded β-ChNF hydrogel activated the TGFβ/smad signaling. The neutralization of TGFβ markedly reduced Smad phosphorylation and the expressions of TIMP1, VEGFR and α-SMA. Taken together, these findings suggest that ADSCs-loaded β-ChNF hydrogel promises for treating wounds that are challenge to heal via conventional methods.

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Section: Discussionmentioning

confidence: 99%

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats

Liu

et al. 2022

J Mater Sci: Mater Med

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Evaluation of New Film Based on Chitosan/Gold Nanocomposites on Antibacterial Property and Wound‐Healing Efficacy

Wang

et al. 2020

Advances in Materials Science and Engineering

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Chitosan (CS) is a natural polymer with applications in wound restorations and coatings for artificial implants. However, the lack of antimicrobial activity restricts its further application. Thus, the purpose of this study is to prepare CS/AuNPs film by adding gold nanoparticles (AuNPs) to modify chitosan. Through transmission electron microscopy, we found that the presence of CS can effectively prevent the agglomeration of nanoparticles. Furthermore, through the CCK experiment, we found that AuNPs at a certain concentration can improve the adhesion and proliferation of cells, which has also been proved in animal experiments, including gross appearance, histological observations, and immunofluorescence staining. Such findings support the feasibility of using the film as a promising candidate for tissue engineering of skin in a near future.

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The use of hardware technologies in the regulation of the wound process in laboratory animals

Prazdnikov¹,

Farhat²,

Evsyukova³

2021

Oper. khir.

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Expression of Metalloproteinases and Type I and III Collagens during Healing of Excisional Skin Wound on the Abdomen and Back in Rats

Cited by 4 publications

References 9 publications

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats

Evaluation of New Film Based on Chitosan/Gold Nanocomposites on Antibacterial Property and Wound‐Healing Efficacy

The use of hardware technologies in the regulation of the wound process in laboratory animals

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