Expression of membrane complement regulators, CD46, CD55 and CD59, in mesothelial cells of patients on peritoneal dialysis therapy

Sei, Yumi; Mizuno, Masashi; Suzuki, Yoshio; Imai, Masaki; Higashide, K.; Harris, Claire L.; Sakata, Fumiko; Iguchi, Daiki; Fujiwara, Michitaka; Kodera, Yasuhiro; Maruyama, Shoichi; Matsuo, Seiichi; Ito, Yasuhiko

doi:10.1016/j.molimm.2015.02.005

Cited by 19 publications

(41 citation statements)

References 31 publications

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“…In PD fluids (PDF) from patients, C proteins such as C3 and C4 were observed [9] and local production was shown from human peritoneal mesothelium under PD [10] suggesting capacity for control of inflammation/infection in peritoneum. We also reported that the CRegs CD46, CD55 and CD59 are broadly distributed in human peritoneum [11], suggesting that the balance of C activation and regulation might be important to maintain homeostasis in human peritoneal cavity. When PD-related peritonitis occurs, activation and/or dysregulation of the C system could be induced in the peritoneum.…”

Section: Introductionmentioning

confidence: 99%

High Levels of Soluble C5b-9 Complex in Dialysis Fluid May Predict Poor Prognosis in Peritonitis in Peritoneal Dialysis Patients

et al. 2017

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BackgroundWe searched for indicators to predict the prognosis of infectious peritonitis by measuring levels of complement proteins and activation products in peritoneal dialysis (PD) fluid (PDF) of patients at early stages of peritonitis. We retrospectively analyzed the relationship between the levels of sC5b-9, C3 and C4 in PDF and the subsequent clinical prognosis.MethodsWe measured levels of sC5b-9, C3 and C4 in PDF on days 1, 2 and 5 post-onset of peritonitis in 104 episodes of infectious peritonitis in PD patients from 2008 and retrospectively compared levels with clinical outcomes. Further analysis for the presence of causative microorganisms or to demonstrate bacterial culture negative peritonitis was performed and correlated with change of levels of sC5b-9 in PDF.ResultsWhen PD patients with peritonitis were divided into groups that either failed to recover from peritonitis and were finally withdrawn from PD (group 1; n = 25) or recovered (group 2; n = 79), levels of sC5b-9, C3 and C4 in PDF were significantly higher in group 1 patients compared to those in group 2 on day5. Analysis of microorganisms showed significantly higher sC5b-9 levels in PDF of peritonitis cases caused by culture negative peritonitis in group 1 compared with group 2 when we analyzed for individual microorganisms. Of note, on day5, the sC5b-9 levels in PDF were similarly high in peritonitis caused by fungi or other organisms.ConclusionOur results suggested that levels of complement markers in PDF, especially sC5b-9, have potential as surrogate markers to predict prognosis of PD-related peritonitis.

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Section: Introductionmentioning

confidence: 99%

High Levels of Soluble C5b-9 Complex in Dialysis Fluid May Predict Poor Prognosis in Peritonitis in Peritoneal Dialysis Patients

et al. 2017

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“…There is growing evidence from animal models describing an important role of a local regulatory CS in the peritoneal cavity and association of disturbed membrane complement regulators (CRegs) with PM injury [34–37]. A recent cell culture study reported an abundantly expression of the CRegs CD46, CD55 and CD59 in human mesothelial cells [38]. Beside these Sei et al showed a modified expression of the CReg CD55 in human mesothelial cells from PD patients with high peritoneal membrane solute transport [38].…”

Section: Introductionmentioning

confidence: 99%

Alteration of membrane complement regulators is associated with transporter status in patients on peritoneal dialysis

et al. 2017

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IntroductionA growing body of evidence from animal models and cell culture studies indicate an important role of a local regulatory complement system (CS) in peritoneal injury during peritoneal dialysis (PD). We investigated the expression of the local regulatory CS (reflected by CD46,CD55,CD59) in the peritoneal tissue of patients with different membrane function characteristics.Patients and methodsBiopsies from the parietal peritoneum were taken from 24 patients on PD, 22 uremic patients prior to PD. PD patients were grouped according to the dialysate-to-plasma ratio of creatinine (D/P Cre) and ratio of dialysate glucose at 4 hours versus dialysate glucose at time zero (D/D0 glucose) into low or low-average peritoneal transport status (L/LA) and high-average or high-transport status (HA/H) groups. CD46, CD55, and CD59 RNA expression were analyzed by real-time polymerase chain reaction (RT-PCR). Further localization of membrane complement regulators (CRegs) and semiquantitatively analysis was done by immunohistochemistry (IHC).ResultsCD46 and CD59 expression were similar in all groups. CD55 expression was significantly decreased in the HA/H group compared to the L/LA group and to uremic controls (p < 0.05 and p = 0.05, respectively). No statistically significant differences in CD46, CD55, and CD55 expression were detected when considering the history of peritonitis. There was no statistically significant correlation between PD duration and the expressions of CD46, CD55, and CD59. IHC revealed strong CD46, CD55, and CD59 expression in mesothelial cells. CD55 and CD59 were additionally detected in the vasculature. Using IHC, CD46 was lower in PD patients compared to uremic controls (p>0.05), but there was no difference between the L/LA compared to the H/HA group. Moreover IHC confirmed decreased expression of CD55 in the HA/H group compared to the L/LA group and uremic controls (p<0.0001 and p = 0.0001, respectively).ConclusionCD55 expression is decreased in patients with fast transporter membrane function, whereas peritonitis and PD duration do not appear to alter CReg expression.

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“…Turk Neph Dial Transpl 2017; 26 (2): [204][205][206][207][208] GiriŞ SAPD (sürekli ayaktan periton diyalizi) tedavisinin en önemli sorunu, bağlantı sistemlerindeki sürekli gelişmeye rağmen oluşan peritonitlerdir (1). Tekrarlayan peritonit atakları peritoneal fibrozis ile sonuçlanmakta ve çoğu hasta bu nedenle hemodiyalize geçiş yapmaktadır.…”

Section: Sarıkaya M Ve Ark: Sapd Hastalarında Peritonitunclassified

“…Tekrarlayan peritonit atakları peritoneal fibrozis ile sonuçlanmakta ve çoğu hasta bu nedenle hemodiyalize geçiş yapmaktadır. Aynı asepsi koşullarının sağlanmasına rağmen bazı hastalarda daha sık peritonit gelişmesi immün sisteme ait faktörlerin önemli olabileceğini düşündürmektedir (2). Normal peritonla karşılaştırıldığında, kronik diyaliz amacıyla kullanılan periton boşluğu, kontamine edici mikroorganizmaların lenfatik yolla temizlenmesinin yetersiz olması ve antikor, kompleman ve lökosit düzeylerinin kritik düzeylere düşmesi nedeniyle bağışıklık açısından zayıf bir bölge olarak kabul edilir.…”

Section: Sarıkaya M Ve Ark: Sapd Hastalarında Peritonitunclassified

“…Normal peritonla karşılaştırıldığında, kronik diyaliz amacıyla kullanılan periton boşluğu, kontamine edici mikroorganizmaların lenfatik yolla temizlenmesinin yetersiz olması ve antikor, kompleman ve lökosit düzeylerinin kritik düzeylere düşmesi nedeniyle bağışıklık açısından zayıf bir bölge olarak kabul edilir. (2). Opsonizasyonda çok önemli olan IgG ve C3 düzeyleri SAPD hastalarının periton sıvısında normal periton sıvısındakinin çok altındaki değerlere düşmektedir (3).…”

Section: Sarıkaya M Ve Ark: Sapd Hastalarında Peritonitunclassified

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Sürekli Ayaktan Periton Diyaliz Hastalarında Peritonit ve Serum-Diyalizat İmmünglobulin-G Subgrup Düzeyleri İlişkisi

Sarıkaya¹,

Şahintürk²,

İnci³

2017

Turk Neph Dial Transpl

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ÖzAMAÇ: Peritonit, Sürekli Ayaktan Periton Diyaliz (SAPD) tedavisinin en önemli komplikasyonudur. Peritoneal savunma sisteminin önemli elementi IgG ve subgruplarıdır. IgG subgrupları eksikliği tekrarlayan infeksiyonlara eğilimle sonuçlanması nedeniyle serum ve diyalizatta IgG subgruplarının düzeylerinin peritonit sıklığı ile ilişkisinin araştırılması amaçlandı. GErEÇ ve yÖntEMLEr:Çalışmaya 30 SAPD hastası alındı. Hastalar yıllık peritonit sıklığına göre iki gruba ayrıldı. Yılda iki veya daha fazla sayıda peritonit atağı geçiren hastalar high peritonitis index (HPI), yılda bir veya hiç peritonit geçirmeyenler ise low peritonitis index (LPI) olarak gruplandırıldı. 17 (%56,67) hasta LPI, 13 (%43,33) hasta HPI grubuna dahil edildi. İki grup IgG subgrup düzeyi açısından karşılaştırıldı.BULGULAr: Her iki grupta serum IgG subgrupları karşılaştırıldığında anlamlı fark saptanmadı (p IgG1:0,86, IgG2:0,93, IgG3:0,4, IgG4:0,67, total IgG:0,80 mg/ml). Diyalizat IgG subgruplarının HPI grubunda anlamlı düzeyde yüksek olduğu görüldü (p IgG1:0,006, IgG2:0,01, IgG3:0,007, IgG4:0,01, total IgG:0,003 mg/ml). HPI grubunda diyalizat total protein, globulin ve albumin kayıp düzeyleri LPI grubuna göre belirgin olarak yüksek bulundu (sırasıyla p=0,39, 0,26, 0,01).SOnUÇ: HPI olgularında IgG subgrupları düzeylerinin LPI olgularına göre yüksek olmasının nedeni, tekrarlayan peritonitler sonucunda periton membranındaki yapısal değişikliklere bağlı artmış makro molekül kaybı ve gelişen inflamatuvar bir yanıt olabilir. Sonuçlarımız IgG subgrup düzeyleri eksikliğinin peritonit gelişiminde önemli bir rol oynamadığını düşündürmektedir.AnAhtAr SÖzcükLEr: Sürekli ayaktan periton diyalizi, Peritonit, IgG subgrupları ABStrAct OBJEctIVE: Peritonitis is the most important complication of Continuous Ambulatory Peritoneal Dialysis (CAPD) treatment. The most powerful defending elements of peritoneal fluid are IgG and the IgG subclasses. Low levels of IgG subclasses result in recurrent infections. We therefore aimed to investigate the peritonitis frequency and serum-dialysate IgG subclass level relationship. MAtErIAL and MEthODS:A total of 30 CAPD patients were included in this study. The patients were divided into two groups according to their peritonitis frequency. Patients with two or more peritonitis attack were grouped as High Peritonitis Index (HPI) and those with one or less peritonitis attack as Low Peritonitis Index (LPI). 17 (56.67%) patients were in the LPI and 13 (43.33%) patients in the HPI group. These two groups were compared for their IgG subclass levels.rESULtS: There was no significant difference for serum IgG and subclass levels (p IgG1:0.86, IgG2:0.93, IgG3:0.4, IgG4:0.67, total IgG:0.80 mg/ml). Dialysate IgG and subclass levels were significantly higher in the HPI group (p IgG1:0.006, IgG2:0.01, IgG3:0.007, IgG4:0.01, total IgG:0.003 mg/ml). The loss of total protein, albumin and globulin in the dialysate was significantly higher in the HPI group (respectively p=0.39, 0.26, 0.01).cOncLUSIOn: Structural changes in the peritoneal membra...

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Expression of membrane complement regulators, CD46, CD55 and CD59, in mesothelial cells of patients on peritoneal dialysis therapy

Cited by 19 publications

References 31 publications

High Levels of Soluble C5b-9 Complex in Dialysis Fluid May Predict Poor Prognosis in Peritonitis in Peritoneal Dialysis Patients

High Levels of Soluble C5b-9 Complex in Dialysis Fluid May Predict Poor Prognosis in Peritonitis in Peritoneal Dialysis Patients

Alteration of membrane complement regulators is associated with transporter status in patients on peritoneal dialysis

Sürekli Ayaktan Periton Diyaliz Hastalarında Peritonit ve Serum-Diyalizat İmmünglobulin-G Subgrup Düzeyleri İlişkisi

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