Expression of membrane and nuclear melatonin receptors in mouse peripheral organs

Naji, Latifa; Carrillo-Vico, Antonio; Guerrero, Juan M.; Calvo, Juan R.

doi:10.1016/j.lfs.2003.08.046

Cited by 99 publications

(71 citation statements)

References 46 publications

(51 reference statements)

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“…Interestingly, expression of this receptor appears to be restricted to the brain and the lungs, in opposite to a wide distribution of MT1 receptors (35). These findings and our results suggest a major role of melatonin in the regulation of the lung immune response.…”

Section: Discussionsupporting

confidence: 58%

Effects of melatonin in an experimental model of ventilator-induced lung injury

Pedreira

García-Prieto

Parra

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

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Melatonin is a free radical scavenger and a broad-spectrum antioxidant and has well-documented immunomodulatory effects. We studied the effects of this hormone on lung damage, oxidative stress, and inflammation in a model of ventilator-induced lung injury (VILI), using 8- to 12-wk-old Swiss mice ( n = 48). Animals were randomized into three experimental groups: control (not ventilated); low-pressure ventilation [peak inspiratory pressure 15 cmH2O, positive end-expiratory pressure (PEEP) 2 cmH2O], and high-pressure ventilation (peak inspiratory pressure 25 cmH2O, PEEP 0 cmH2O). Each group was divided into two subgroups: eight animals were treated with melatonin (10 mg/kg ip, 30 min before the onset of ventilation) and the remaining eight with vehicle. After 2 h of ventilation, lung injury was evaluated by gas exchange, wet-to-dry weight ratio, and histological analysis. Levels of malondialdehyde, glutathione peroxidase, interleukins IL-1β, IL-6, TNF-α, and IL-10, and matrix metalloproteinases 2 and 9 in lung tissue were measured as indicators of oxidation status, pro-/anti-inflammatory cytokines, and matrix turnover, respectively. Ventilation with high pressures induced severe lung damage and release of TNF-α, IL-6, and matrix metalloproteinase-9. Treatment with melatonin improved oxygenation and decreased histological lung injury but significantly increased oxidative stress quantified by malondialdehyde levels. There were no differences in TNF-α, IL-1β, IL-6, or matrix metalloproteinases caused by melatonin treatment, but IL-10 levels were significantly higher in treated animals. These results suggest that melatonin decreases VILI by increasing the anti-inflammatory response despite an unexpected increase in oxidative stress.

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Section: Discussionsupporting

confidence: 58%

Effects of melatonin in an experimental model of ventilator-induced lung injury

Pedreira

García-Prieto

Parra

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Following MLT treatment, HSP70 protein levels decreased in the hepatic tissue nuclear fraction but were elevated in its cytosolic fraction, while no effect of MLT in the brain tissue was noted. MLT exerts its cellular effect via binding specific receptors (Dubocovich, 1995); the distribution and actions of these receptors vary between tissues (Naji et al, 2004;Sallinen et al, 2005). This may explain the difference in the effect of MLT between tissues.…”

Section: Discussionmentioning

confidence: 99%

Light Interference (LI) as a possible stressor altering HSP70 and its gene expression levels in brain and liver tissues of Golden Spiny Mice

Ashkenazi

Haim

2012

Journal of Experimental Biology

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SUMMARYLight at night and light interference (LI) disrupt the natural light:dark cycle, causing alterations at physiological and molecular levels, partly by suppressing melatonin (MLT) secretion at night. Heat shock proteins (HSPs) can be activated in response to environmental changes. We assessed changes in gene expression and protein level of HSP70 in brain and hepatic tissues of golden spiny mice (Acomys russatus) acclimated to LI for two (SLI), seven (MLI) and 21 nights (LLI). The effect of MLT treatment on LI-mice was also assessed. HSP70 levels increased in brain and hepatic tissues after SLI, whereas after MLI and LLI, HSP70 decreased to control levels. Changes in HSP70 levels as a response to MLT occurred after SLI only in hepatic tissue. However, hsp70 expression following SLI increased in brain tissue, but not in hepatic tissue. MLT treatment and SLI caused a decrease in hsp70 levels in brain tissue and an increase in hsp70 in hepatic tissue. SLI acclimation elicited a stress response in A. russatus, as expressed by increased HSP70 levels and gene expression. Longer acclimation decreases protein and gene expression to their control levels. We conclude that for brain and hepatic tissues of A. russatus, LI is a short-term stressor. Our results also revealed that A. russatus can acclimate to LI, possibly because of its circadian system plasticity, which allows it to behave both as a nocturnal and as a diurnal rodent. To the best of our knowledge, this is the first study showing the effect of LI as a stressor at the cellular level, by activating HSP70. Supplementary material available online at

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“…MT 1 receptor levels were increased in cerebral blood vessels of subjects with Alzheimer's disease (Savaskan et al, 2001). Mouse and rat brain (e.g., hippocampus), retina, and peripheral tissues such as heart, lung, liver, and kidney (Naji et al, 2004;Sallinen et al, 2005) express mRNA for both MT 1 and MT 2 melatonin receptors. mRNAs for both receptor types also have been demonstrated in rat vasculature, where MT 1 receptors mediate vasoconstriction and MT 2 receptors mediate vasodilation (Doolen et al, 1998;Masana et al, 2002).…”

Section: Melatonin Receptor-mediated Functional Responses In Native Tmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. LXXV. Nomenclature, Classification, and Pharmacology of G Protein-Coupled Melatonin Receptors

et al. 2010

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Expression of membrane and nuclear melatonin receptors in mouse peripheral organs

Cited by 99 publications

References 46 publications

Effects of melatonin in an experimental model of ventilator-induced lung injury

Effects of melatonin in an experimental model of ventilator-induced lung injury

Light Interference (LI) as a possible stressor altering HSP70 and its gene expression levels in brain and liver tissues of Golden Spiny Mice

International Union of Basic and Clinical Pharmacology. LXXV. Nomenclature, Classification, and Pharmacology of G Protein-Coupled Melatonin Receptors

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