Expression of matrix metalloproteinases in the subretinal fluid correlates with the extent of rhegmatogenous retinal detachment

Symeonidis, Chrysanthos; Diza, Eudoxia; Papakonstantinou, Eleni; Souliou, Efimia; Karakiulakis, George; Dimitrakos, Stavros

doi:10.1007/s00417-006-0386-3

Cited by 24 publications

(18 citation statements)

References 25 publications

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“…A previous study using an animal retinal detachment model showed that subretinal fluid interferes with normal diffusion of oxygen and nutrients from the retinal pigment epithelium to photoreceptors and thus could induce photoreceptor degeneration [12]. Moreover, the high concentration of degradative enzymes in the subretinal fluid might have resulted in something more than simple diffusion-blockage in rhegmatogenous retinal detachment [14,15]. Second, in the three cases, there might have been additional defects in structural factors in the subretinal space which could not be detected with SD-OCT, i.e., connections between photoreceptor and retinal pigment epithelium or interphotoreceptor matrix [16].…”

Section: Discussionmentioning

confidence: 99%

Photoreceptor Disruption Related to Persistent Submacular Fluid after Successful Scleral Buckle Surgery

Woo

Lee

Chung

et al. 2011

Korean J Ophthalmol

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PurposeTo investigate serial changes in photoreceptor status and associated visual outcome in patients with persistent submacular fluid after successful scleral buckle surgery for macula-off rhegmatogenous retinal detachment.MethodsThis was a prospective observational case series including 76 consecutive patients who underwent successful scleral buckle surgery for macula-off rhegmatogenous retinal detachment with symptom duration ≤90 days at a single tertiary hospital. Optical coherence tomography (OCT) and visual acuity examination were performed at one month and three months postoperatively and at three-month intervals until the submacular fluid disappeared. Main outcome measures were postoperative photoreceptor status on OCT and visual acuity.ResultsForty-two patients (55.3%) showed persistent submacular fluid at postoperative one month. Of 42 patients with persistent submacular fluid, three (7.1%) showed photoreceptor disruption on OCT. None of the 34 patients without persistent submacular fluid showed photoreceptor disruption. Two patients (4.8%) had progressive photoreceptor disruption, and one patient (2.4%) had early photoreceptor disruption. All three patients showed photoreceptor reappearance and limited visual restoration after absorption of submacular fluid. Final visual acuities were significantly worse in these three patients (20 / 1000, 20 / 133, and 20 / 133) compared to those of the other patients (mean, 20 / 30) with persistent submacular fluid and intact photoreceptors.ConclusionsEven after successful scleral buckle surgery for rhegmatogenous retinal detachment, photoreceptor disruption can occur related to persistent submacular fluid and may be a cause of poor visual outcome.

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Section: Discussionmentioning

confidence: 99%

Photoreceptor Disruption Related to Persistent Submacular Fluid after Successful Scleral Buckle Surgery

Woo

Lee

Chung

et al. 2011

Korean J Ophthalmol

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“…MMP gelatinolytic activity was determined by gelatin zymography analysis using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) under denaturing but non‐reducing conditions, as described previously (Karakiulakis et al. 1997; Symeonidis et al. 2007b).…”

Section: Methodsmentioning

confidence: 99%

“…We have shown previously that a regression model consisting of MMP‐3, MMP‐8 and proMMP‐9 may be of use in providing new information for the evaluation of RRD extent (Symeonidis et al. 2007b).…”

Section: Introductionmentioning

confidence: 99%

Correlation of matrix metalloproteinase levels with the grade of proliferative vitreoretinopathy in the subretinal fluid and vitreous during rhegmatogenous retinal detachment

Symeonidis¹,

Papakonstantinou²,

Souliou³

et al. 2011

Acta Ophthalmologica

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. Purpose: We investigated the activity of matrix metalloproteinase (MMP)‐2 and ‐9 and their latent pro‐forms (proMMP‐2, ‐9), and protein levels of MMP‐1, ‐3, ‐8 and tissue inhibitor of MMPs (TIMP)‐1 in the subretinal fluid (SRF) and vitreous of patients with rhegmatogenous retinal detachment (RRD). Potential correlations with proliferative vitreoretinopathy (PVR) grade were determined. Methods: Thirty‐seven SRF and 32 vitreous samples from RRD patients and nine vitreous samples from human organ donors (controls), were collected and assayed for MMP‐1, ‐3, ‐8/TIMP‐1 levels using enzyme‐linked immunosorbent assay (ELISA), and for proMMP‐2, ‐9, MMP‐2, ‐9 activity employing gelatine zymography. Results: ProMMP‐2, ‐9, MMP‐1, ‐3, ‐9, TIMP‐1 were significantly higher in the SRF and vitreous of RRD patients compared to the vitreous of organ donors. MMP‐8 levels were higher in RRD patients’ SRF. Regarding PVR grade, MMPs and TIMP‐1 were differentially present in SRF and vitreous. PVR grade correlated significantly with the levels of MMP‐2 in SRF, while proMMP‐2, MMP‐1, ‐2, ‐3, ‐8, ‐9 and TIMP‐1 levels correlated with PVR grade in the vitreous. Conclusion: MMP/TIMP‐1 levels are elevated in SRF and vitreous during RRD. Significant correlations between PVR grade and MMP‐2 in SRF and proMMP‐2, MMP‐1, ‐2, ‐3, ‐8, ‐9 and TIMP‐1 levels in vitreous were revealed. Investigation of MMP activity in vitreous may provide more valid conclusions compared to SRF pertaining to the role of the MMPs during RRD. The observations of the present study suggest a possible role for MMPs and TIMP‐1 in PVR pathophysiology.

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“…epiretinal membranes). 14 RRD appears to be an environment of stimuli for the production of a variety of factors with far-ranging activities and functions. In a study by LaHeij et al, basic fibroblast growth factor (BFGF), glutamine synthetase, and interleukin (IL)-6 were found to be elevated in the SRF during RRD.…”

Section: Rpe Cells Have Been Shown To Be Constituents Of the Srf In Rmentioning

confidence: 99%

Chemokine CXCL-1 Expression in the Subretinal Fluid during Rhegmatogenous Retinal Detachment

Symeonidis

Androudi

Rotsos

et al. 2013

Ocular Immunology and Inflammation

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Expression of matrix metalloproteinases in the subretinal fluid correlates with the extent of rhegmatogenous retinal detachment

Abstract: MMPs and TIMP-1 levels are elevated in SRF during RRD. A regression model consisting of MMP-3, MMP-8 and proMMP-9 may be proved to be of potential use in providing information for evaluation of the extent of RRD.

Cited by 24 publications

References 25 publications

Photoreceptor Disruption Related to Persistent Submacular Fluid after Successful Scleral Buckle Surgery

Photoreceptor Disruption Related to Persistent Submacular Fluid after Successful Scleral Buckle Surgery

Correlation of matrix metalloproteinase levels with the grade of proliferative vitreoretinopathy in the subretinal fluid and vitreous during rhegmatogenous retinal detachment

Chemokine CXCL-1 Expression in the Subretinal Fluid during Rhegmatogenous Retinal Detachment

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