Expression of matrix metalloproteinases during murine chorioallantoic placenta maturation

Teesalu, Tambet; Masson, Régis; Basset, Paul; Blasi, Francesco; Talarico, Daniela

doi:10.1002/(sici)1097-0177(199903)214:3<248::aid-aja8>3.0.co;2-n

Cited by 46 publications

(35 citation statements)

References 43 publications

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“…However, we studied only the effect of high glucose on the trophoblast invasive phenotype, whilst maternal diabetes might involve other factors likely to influence gelatinase B activity. Furthermore, several studies have shown that the mechanisms by which trophoblast cells invade basement in vitro could implicate other effectors that gelatinase B [38,39,40]. For example studies investigating the tissue inhibitors of metalloproteinases (TIMPs) gene expression in vivo showed that concentrations of TIMP-1 mRNA peak in mouse uterus, decidua and placenta in the period covering days 6 to 10 after fertilization, the most invasive period of implantation [41].…”

Section: Discussionmentioning

confidence: 99%

Effect of high concentrations of glucose on differentiation of rat trophoblast cells in vitro

et al. 2003

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Aims/hypothesis. Previous studies have shown that diabetic placentas are characterized by structural and biochemical anomalies, including defects in the differentiation of trophoblasts. In this study, the Rcho-1 cell line was used to investigate the impact of high glucose concentrations on different markers of differentiation of rat trophoblast cells in giant cells (endoreduplication, invasive phenotype and endocrine phenotype). Materials. Rcho-1 cells were incubated for 12 days in medium supplemented with different concentrations of glucose and 10% horse serum to optimize differentiation. The cells were examined for the proportion of nuclei showing signs of apoptosis. The effect of high glucose was investigated on the endoreduplication process, on invasive phenotype (secretion of gelatinase B) and on endocrine phenotype (expression of placental lactogen I (PL-I) and II (PL-II) and progesterone secretion). Results. Apoptosis was not induced by high glucose in Rcho-1. The number of cells was higher in the cultures exposed to high glucose (p<0.05) and their nuclei contained more DNA compared with control cells (p<0.001), while their nuclear size was smaller (p<0.001). Gelatinase B secretion increased during differentiation but no difference was found when gelatinase B secretion from trophoblasts exposed to high glucose was compared with the control cells. Rcho-1 cell cultures showed an increase in PL-I and PL-II mRNA expressions during differentiation and which was not affected by high glucose. Progesterone secretion increased during differentiation in control cultures. However, this increase was abolished when trophoblasts were cultured in high glucose. Conclusions/interpretation. Our data suggest that high glucose influences the endoreduplication process and the steroidogenesis during differentiation of rat trophoblasts. [Diabetologia (2003) 46:276-283]

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Section: Discussionmentioning

confidence: 99%

Effect of high concentrations of glucose on differentiation of rat trophoblast cells in vitro

et al. 2003

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“…TGCs secrete a variety of proteinases that are thought to digest the ECM as well as phagocytosed maternal cells and matrix materials. They include matrix metalloproteinases (MMP-2, -3, -9, -13) and inhibitors of metalloproteinases (TIMP-1, -2, -3, -4) (Alexander et al, 1996;Das et al, 1997;Harvey et al, 1995;Teesalu et al, 1999;Zhang et al, 2003) urokinase plasminogen activators (Teesalu et al, 1998;Teesalu et al, 1999) and cathepsins (Afonso et al, 1999;Deussing et al, 2002;Hemberger et al, 2000;Ishida et al, 2004).…”

Section: Invasion Into the Decidua And Anastomosis With The Maternal mentioning

confidence: 99%

Development and function of trophoblast giant cells in the rodent placenta

Hu¹,

Cross²

2010

Int. J. Dev. Biol.

264

247

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“…The broad expression could indicate a regulatory function in finetuning the activity of MMPs such as MMP1, MMP2 and MMP3. It has been shown for TIMP3 that expression is strictly balanced with expression of MMP9 during early gestation in the mouse (Teesalu et al 1999). The process of parturition is also associated with the regulation of vascular remodelling-related genes like SERPINE1, FBLN5, thrombospondin 1 (THBS1; 2.6-fold; Haddad et al 2008), angiopoietin 2 (ANGPT2; 4.4-fold) and angiopoietin-like 4 (ANGPTL4; 2.7-fold).…”

Section: Genes Related To Immune Responsementioning

confidence: 99%

Gene expression profiling of bovine peripartal placentomes: detection of molecular pathways potentially involved in the release of foetal membranes

Streyl¹,

Kenngott²,

Herbach³

et al. 2012

Reproduction

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The mechanisms underlying detachment of foetal membranes after birth in cows are still unclear. To address this problem in a systematic manner, we performed the first holistic transcriptome study of bovine placentomes antepartum (AP; nZ4 cows) and intrapartum (IP; nZ4 cows) using Affymetrix GeneChip Bovine Genome Arrays. Three placentomes were extracted from each cow, and tissue samples from the contact zones of the placentomes (foeto-maternal units) were recovered by systematic random sampling and processed for RNA extraction and for stereological quantification of cellular composition. Statistical analysis of microarray data (false discovery rate 1%) revealed 759 mRNAs with at least twofold higher levels in the samples of the AP group, whereas 514 mRNAs showed higher levels in the IP group. The differentially expressed genes were classified according to biological processes and molecular functions using the Functional Annotation Clustering tool of the DAVID Bioinformatics Resources. Genes with higher mRNA levels in the AP group were nearly completely related to mitotic cell cycle and tissue differentiation. During parturition, a complete shift occurred because the genes with higher mRNA levels in IP were nearly all related to three different physiological processes/complexes: i) apoptosis, ii) degradation of extra cellular matrix and iii) innate immune response, which play a fundamental role in placental detachment. These results are an excellent basis for future studies investigating the molecular basis of retained foetal membranes.

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Expression of matrix metalloproteinases during murine chorioallantoic placenta maturation

Cited by 46 publications

References 43 publications

Effect of high concentrations of glucose on differentiation of rat trophoblast cells in vitro

Effect of high concentrations of glucose on differentiation of rat trophoblast cells in vitro

Development and function of trophoblast giant cells in the rodent placenta

Gene expression profiling of bovine peripartal placentomes: detection of molecular pathways potentially involved in the release of foetal membranes

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