Expression of Matrix Metalloproteinases and Theirs Tissue Inhibitors in Fibroblast Cultures and Colo-829 and SH-4 Melanoma Cultures After Photodynamic Therapy

Zielińska, Aleksandra; Latocha, Małgorzata; Jurzak, Magdalena; Kuśmierz, Dariusz

doi:10.5772/54935

Cited by 3 publications

(3 citation statements)

References 59 publications

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“…For tissue invasion to occur, it is necessary that the MMPs cleave the basal membrane and the extracellular matrix, allowing the migration of these cells to the bloodstream [ 85 ]. Twenty-five MMPs have been characterized, as well as 4 of its inhibitors (TIMPs) [ 86 ]. The expression of MMPs can be activated by cytokines and growth factors, including TGF- α and β , interleukins, TNF- α , interferons, FGF, and VEGF [ 87 ].…”

Section: Discussionmentioning

confidence: 99%

“…Together with its inhibitors, such as TIMP-1 and TIMP-2, the MMPs keep a balance between collagen production and degradation. Each of these proteinases have specific roles in determining tumor invasive capacity [ 86 ]. MMP-2 and MMP-9 are especially relevant in melanoma progression, since they play an important role in tumor development, growth, angiogenesis, and metastasis.…”

Section: Discussionmentioning

confidence: 99%

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Applicability of Plant Extracts in Preclinical Studies of Melanoma: A Systematic Review

Albuquerque

Pacheco

Casao

et al. 2018

Mediators of Inflammation

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Melanoma is the most aggressive form of skin cancer and arises from melanocyte gene mutation. This disease is multifactorial, but its main cause is the excessive exposure to ultraviolet radiation. Currently, available chemotherapy has shown little expressive results, which may justify the high use of natural products to treat this cancer. We performed a systematic review to compile the results of studies carried out in murine models and investigated the effect of plant extracts on melanoma treatment. Papers were selected in MEDLINE/Pubmed and Scopus according to the PRISM statement. Search filters were developed using three parameters: plant extract, melanoma, and animal model. The 35 identified studies were all submitted to the criteria described in the ARRIVE guidelines. The different extracts showed antiangiogenic, antimetastatic, antioxidant, and anti-inflammatory activity, and also proved to be effective in cell cycle modulation and apoptosis evasion. Bias analysis evidenced the absence of standardized experimental designs, as well as failures in statistical tests and in the presentation of results. The analysis of the studies suggests that the use of plant extracts is effective for the treatment of melanoma in murine models.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Applicability of Plant Extracts in Preclinical Studies of Melanoma: A Systematic Review

Albuquerque

Pacheco

Casao

et al. 2018

Mediators of Inflammation

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“…Gene expression of metalloproteinases is detected in particularly all cell types such as fibroblasts, keratinocytes, macrophages, endothelium cells, Langerhans dendritic cells, neurons, microglial cells, myocytes and in inflammatory infiltration cells (monocytes and T lymphocyte). [ 11 ] There is an abundant amount of evidence that MMPs and their endogenous TIMPs are over expressed in various tumour cells and tissues and pay key role in the process of cancer development and progression. [ 7 , 12 , 13 ]…”

Section: Introductionmentioning

confidence: 99%

Investigation of the role ofMMP3 -1171insApolymorphism in cutaneous malignant melanoma – a preliminary study

Vlaykova

Kurzawski

Tacheva

et al. 2014

Biotechnology & Biotechnological Equipment

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Coetaneous malignant melanoma is the most aggressive cancer of the skin with a high rate of mortality worldwide. Degradation of basement membranes and extracellular matrix is an essential step in cancer invasion and metastasis. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play key roles in this step. MMP-3 also called stromelysin-1 was one of the first proteinases found to be associated with cancer. In the gene of MMP-3 (MMP3), an insertion/deletion of an A nucleotide at position -1171 in promoter region has been identified and shown to effect the expression activity of the gene.The present study was conducted to investigate the relation of MMP3 -1171insA polymorphism with skin malignant melanoma risk in a pilot case-control study of Bulgarian patients (n = 26) and unaffected controls (n = 172).The genotypes of controls and melanoma patients were in Hardy-Weinberg equilibrium. The results showed no statistically significant difference both in genotype and allele frequencies of MMP3 -1171insA polymorphism between melanoma patients and healthy controls either in crude analyses (p = 0.360 and 0.790, c2-test) or after adjustment for age and sex. The comparison of some clinical characteristics between the patients with different genotypes showed a trend for longer survival of patients with 6A/6A genotype compared to the carriers of 5A allele (5A/5A+5A/6A genotypes, p = 0.118, Log rank test).The results of our current preliminary study do not provide evidence for the role of the promoter polymorphism -1171insA in MMP3 as a risk factor for development of coetaneous melanoma, but suggest its implication in progression of the diseases.

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New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma

et al. 2023

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Melanoma is a form of skin cancer that can rapidly invade distant organs. A distinctive feature of melanomas is their pigmentation status, as melanin is present in most skin melanomas, whilst many metastatic tumors could become amelanotic. Besides the obvious malfunction of the key genes of the melanin pathway, the amelanotic tumors could bear a characteristic molecular signature accounting for their aggressivity. Using mass spectrometry-based proteomics we report here a distinctive panel of biomarkers for amelanotic aggressive melanoma that differ from the less invasive pigmented cells. The developed method allows the label-free quantification of proteins identified by LC-MS/MS analysis. We found a set of proteins comprising AHNAK, MYOF, ANXA1, CAPN2, ASPH, EPHA2, THBS1, TGM2, ACTN4 along with proteins involved in cell adhesion/migration (integrins, PLEC, FSCN1, FN1) that are highly expressed in amelanotic melanoma. Accompanying the down regulation of pigmentation specific proteins such as tyrosinase and TYRP1, these biomarkers are highly specific for a type of highly invasive melanoma. Interestingly, the LC-MS/MS proteomics analysis in hypoxia revealed that the abundance of this specific set of proteins found in normoxia was rather unaltered in these conditions. These biomarkers could therefore predict a metastatic behaviour for the amelanotic cells in the early stages of the tumor development and thus serve in melanoma prognostic. Applying this algorithm to related databases including melanoma samples published by independent laboratories/public databases we confirm the specificity of the newly found signatures. Overall, we begin to unravel the molecular alterations in the amelanotic melanoma and how basic proteomics offers insights into how to assess the clinical, pathological and misdiagnosis differences between the main subtypes of melanoma.

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Expression of Matrix Metalloproteinases and Theirs Tissue Inhibitors in Fibroblast Cultures and Colo-829 and SH-4 Melanoma Cultures After Photodynamic Therapy

Cited by 3 publications

References 59 publications

Applicability of Plant Extracts in Preclinical Studies of Melanoma: A Systematic Review

Applicability of Plant Extracts in Preclinical Studies of Melanoma: A Systematic Review

Investigation of the role ofMMP3 -1171insApolymorphism in cutaneous malignant melanoma – a preliminary study

New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma

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