Expression of matrix metalloproteinase-9 and its substrate level in patients with premature rupture of membranes

Abstract

In this study, 30 case of patients with full-term premature membrane rupture and another 30 cases of full-term delivered subject without premature rupture of membranes (PROM) were selected to explore the relationship between premature membrane rupture with matrix metalloproteinase 9 (MMP-9) and its substrate level. Results showed the plasma zinc, MMP-9 in serum and amniotic fluid increased in patients with PROM; their type IV collagen in serum and foetal membrane decreased. Increased Zinc ion concentration results in increased concentration of MMP-9, a zinc-dependent enzyme; the degradation of type IV collagen by MMP-9 might be the potential mechanism of premature rupture of membranes in full-term pregnant women.

Determinants of premature rupture of membrane in Southern Ethiopia, 2017: case control study design

“…In case of hypertension during pregnancy, there is reduced uteroplacental perfusion as a result of abnormal cytotrophoblast invasion of spiral arterioles and endothelial dysfunction and in turn placental ischemia which brings premature rupture of membrane [31, 32]. This premature rupture of membranes in pregnant women can be happen due to type IV collagen reduction in serum and fetal membrane by Matrix Metalloproteinase-9 [33], and increased cytokine concentrations that may contribute to the endothelial damage [34].…”

MicroRNAs miR-4535 and miR-1915-5p in Amniotic Fluid As Predictive Biomarkers for Chorioamnionitis

“…The expression of miR-4535 and miR-1915-5p in the amniotic fluid was found to be related to the fetal and maternal inflammatory responses. Intense inflammatory responses are mediated by various cytokines or inflammatory products, including IL-1β, TNF-α, IL-6, PAF, IFN-γ, PGE2, MMP-1 and MMP-2 [19]. In particular, the fetal inflammatory response syndrome is characterized by systemic future science group 10.2144/fsoa-2021-0006 inflammation and elevated fetal plasma IL-6 levels [62].…”

“…In addition, among the infection of miCAM (U. parvum, S. agalactiae, G. vaginalis, S. sanguinegens, P. bivia, L. jensenii and M. hominis), we have previously reported that 33 of 43 (77%) patients with severe chorioamnionitis were identified as having U. parvum in amniotic fluid, using a next-generation sequencing method [13]. In contrast, inflammatory changes have a greater association with adverse perinatal outcomes than the presence of the aforementioned microorganisms [18][19][20][21], thus promising predictive biomarkers for chorioamnionitis must be identified in host inflammatory reactions and via absolute quantification and sequencing of 16S rDNA.…”

Pregnancy complications, treatment characteristics and birth outcomes in women with atopic dermatitis in Denmark

“…Over expression of matrix metallopeptidase-9 (MMP-9) causing degradation of the extracellular matrix within the amniotic membrane has been suggested as a possible mechanism. 39,40 MMP-9 has also been implicated as part of the pro-inflammatory milieu and serum MMP-9 is indeed elevated in AD patients. 41 No other obstetric outcomes were clearly associated with maternal AD.…”

“…However, the association did not meet statistical significance in sub‐analysis. Over expression of matrix metallopeptidase‐9 (MMP‐9) causing degradation of the extracellular matrix within the amniotic membrane has been suggested as a possible mechanism . MMP‐9 has also been implicated as part of the pro‐inflammatory milieu and serum MMP‐9 is indeed elevated in AD patients .…”