Expression of Matrix Metalloproteinase-2, but not Caspase-3, Facilitates Distinction between Benign and Malignant Thyroid Follicular Neoplasms

Sanii, Sanaz; Saffar, Hiva; Tabriz, Hedieh Moradi; Qorbani, Mostafa; Haghpanah, Vahid; Tavangar, Seyed Mohammad

doi:10.7314/apjcp.2012.13.5.2175

Cited by 28 publications

(28 citation statements)

References 28 publications

(39 reference statements)

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“…Caspase-3: sections were subjected to staining with the primary rabbit polyclonal anti-caspase-3 antibody (Thermo Scientific, Lab Vision, USA) [20] .…”

Section: Immunohistochemical Studymentioning

confidence: 99%

The Possible Protective Role of Melatonin on Doxorubicin Induced Cardiomyopathy of Adult Male Albino Rats

Yassien

Elsaid

2017

Egyptian Journal of Histology

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Introduction: Doxorubicin is one of the major antitumor treatment. The essential limiting factor of using this drug is the production of cardiotoxicity. However, melatonin is a powerful antioxidant that may protect the heart. Aim: This study was aimed to study possible protective role of melatonin in adult male albino rats following doxorubicin administration Material and Method: In this study, 40 adult male albino rats were used. They were divided into four groups (10 rats for each): control group, Melatonin group, Doxorubicin group and Doxorubicin & Melatonin group. Heart specimens were obtained at the end and processed. Results: Light studies showed degenerative changes. Some fibers showed dark acidophilic cytoplasm and pyknotic nuclei. Apoptosis were detected where the nuclei varying from peripheral condensation of chromatin up to pyknosis, confirmed with positively caspase-3 activity. Dilatation of the vessels with mononuclear cellular infiltrations and deposited collagen fibers were seen. Ultrastructural examination showed disarrangement of the sarcomeres, disruption of microfilaments and Z-line, the number and size of mitochondria apparently increased, dilated SER and T tubules were also noticed. The presence of oval shaped cells (Telocyte) with thin long processes (Telopodes) were detected. Conclusion: From this study, it was concluded that, melatonin markedly suppressed cardiomyopathy induced by doxorubicin.

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“…Caspase-3: sections were subjected to staining with the primary rabbit polyclonal anti-caspase-3 antibody (Thermo Scientific, Lab Vision, USA) [20] .…”

Section: Immunohistochemical Studymentioning

confidence: 99%

The Possible Protective Role of Melatonin on Doxorubicin Induced Cardiomyopathy of Adult Male Albino Rats

Yassien

Elsaid

2017

Egyptian Journal of Histology

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“…So, it might the high expression of MMPs genes allow the remodeling of extracellular matrix to increase the invasive potential of cancer cells (Gonzalez-Arriaga et al, 2012). MMP-2 is known as gelatinase A that degrades the type IV collagen and gelatin, which are the main constituents of basal lamina and might affect early events in carcinogenesis and tumor invasion and/or metastasis (Sanii et al, 2012). The MMP-2 gene locates on chromosome 16 at q13-21 and has thirteen exons (Saeed et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The MMP-2 -735 C Allele is a Risk Factor for Susceptibility to Breast Cancer

Yari¹,

Rahimi²,

Moradi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: The expression of MMP genes has been demonstrated to be associated with tumor invasion, metastasis and survival rate for a variety of cancers. The functional promoter polymorphism MMP-2 C-735T is associated with decreased expression of the MMP-2 gene. The aim of present study was to detect any association between MMP-2 C-735T and susceptibility to breast cancer. Materials and Methods: The MMP-2 C-735T polymorphism was studied in 233 women (98 with breast cancer and 135 healthy controls). All studied women were from Kermanshah and Ilam provinces of Western Iran. The MMP-2 C-735T polymorphism was detected using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: The frequencies of MMP-2 CC, CT and TT genotypes in healthy individuals were 59.3, 38.5 and 2.2%, respectively. However, in breast cancer patients, only CC (71.4%) and CT (28.6%) genotypes were observed (p=0.077). controls (78.5 %, p=0.048). The presence of C allele of MMP-2 increased the risk of breast cancer by 1.64-fold family history of cancer (67.3%, p=0.31). Conclusions: polymorphism is associated with increased risk of breast cancer. Also, the MMP-2 C allele might increase the risk of young onset breast cancer in our population.

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“…[2] Thyroid lesions with follicular growth pattern are composed of a wide range including benign adenomatous nodule, Follicular Thyroid Adenoma (FTA), Follicular Thyroid carcinoma (FTC) and Follicular variant of Papillary Thyroid Carcinoma (FVPTC). [3][4][5] FTA, FTC and FVPTC are well encapsulated lesions sharing many imaging, cytologic and histologic features [5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

“…[5,10] One of the most challenging tasks in pathologic evaluation of thyroid follicular pattern lesion is distinction between FTC and FTA as a common benign thyroid nodule. [4] Diagnosis of carcinoma is based on the presence of capsular, vascular or extra thyroid tissue invasion or nodal or distant metastasis. [5,8,[10][11][12] Different tools such as IHC and molecular profiling have been used to differentiate between benign and malignant follicular neoplasms or differentiating malignant tumor subtypes, [4,9,13] including some angiogenesis factors.…”

Section: Introductionmentioning

confidence: 99%

“…[4] Diagnosis of carcinoma is based on the presence of capsular, vascular or extra thyroid tissue invasion or nodal or distant metastasis. [5,8,[10][11][12] Different tools such as IHC and molecular profiling have been used to differentiate between benign and malignant follicular neoplasms or differentiating malignant tumor subtypes, [4,9,13] including some angiogenesis factors. [9] Thyroid is rich in vascular network [14] and shows increased vascularity in both non neoplastic and malignant conditions.…”

Section: Introductionmentioning

confidence: 99%

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Expression of PSMA in Thyroid follicular neoplasms: Utility for differentiating between benign from malignant lesions

Saffar

kiany

Tavangar

et al. 2017

APALM

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Background: Prostate-Specific Membrane Antigen (PSMA) has been known as a tumor associated neovasculature marker in some solid malignant tumors. Targeting of PSMA by cytotoxic-conjugated antibody can represent powerful tool for vascular targeted therapy. In thyroid, it appears that angiogenesis factors are involved in neoplastic growth and aggressiveness of tumors.This study was conducted to evaluate the expression of PSMA as an angiogenesis factor in neovasculature of thyroid follicular neoplasms by immunohistochemistry to determine its usefulness for distinguishing between adenoma and carcinoma.Methods: Paraffin Blocks of formalin fixed samples representing 48 cases of Follicular Thyroid Adenoma (FTA) and 15 cases of Follicular Thyroid Carcinoma (FTC) were evaluated.Results: Among 15 cases of FTC, 11 cases revealed both capsular and vascular invasion. In one case only capsular and in two cases only vascular invasions were observed. One case also revealed suspicious vascular invasion with further bone metastasis.The intensity of PSMA staining in vascular structures of carcinomas were significantly higher in comparison with thyroid follicular adenomas (p=0.028). However, we observed no significant difference in the extent of PSMA expression in adenoma and carcinoma groups (p=0.239).Thyroid follicular cells were negative for PSMA in adenoma, carcinoma and in non-neoplastic area. Conclusion:According to our findings, significant higher intensity of PSMA expression in FTC could be useful in differentiating between FTA and FTC. However, more importantly, expression in neovasculature of differentiated thyroid tumors can represent a potential utility for PSMA-targeted radionuclide therapy in advanced differentiated thyroid cancers.

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Expression of Matrix Metalloproteinase-2, but not Caspase-3, Facilitates Distinction between Benign and Malignant Thyroid Follicular Neoplasms

Cited by 28 publications

References 28 publications

The Possible Protective Role of Melatonin on Doxorubicin Induced Cardiomyopathy of Adult Male Albino Rats

The Possible Protective Role of Melatonin on Doxorubicin Induced Cardiomyopathy of Adult Male Albino Rats

The MMP-2 -735 C Allele is a Risk Factor for Susceptibility to Breast Cancer

Expression of PSMA in Thyroid follicular neoplasms: Utility for differentiating between benign from malignant lesions

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