Expression of Lymphocyte Function-Associated Antigen (LFA)-1 in B-Cell Chronic Lymphocytic Leukemia

Woessner, S; Asensio, A.; Florensa, Lourdes; Pedro, C.; Besses, Carles; Sans-Sabrafén, J

doi:10.3109/10428199409049635

Cited by 15 publications

(10 citation statements)

References 15 publications

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“…This contrasted to the more homogeneous distribution in terms of fluorescence intensity of these markers. The expression of these molecules is decreased in CLL, 23 especially in association with a 11q deletion. 12 Due to the important variations of percentage of CD11a, CD11c and CD18-positive cells (the significance of these variations is unknown), these data have to be interpreted with caution; their level of expression would certainly be a more reproducible and useful parameter for the differential diagnosis of chronic lymphoproliferative disorders.…”

Section: Table 4bmentioning

confidence: 99%

Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

et al. 2001

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Integration of morphological and immunophenotypic data is critical in achieving diagnosis accuracy and minimising interobserver interpretative discrepancies. The aim of this work was to compare the immunophenotype and the morphology of chronic lymphocytic leukaemia and mantle cell lymphoma, to help in the differential diagnosis of CD5 positive monoclonal B cells. Frozen/thawed samples from 91 patients were analysed retrospectively. Fresh samples from 17 mixed/atypical CLL and 13 MCL were tested to corroborate the results. Markers were analysed as percentage (%) of positive B lymphocyte subpopulation, and in terms of median fluorescence intensity (MFI). Matutes's CLL score clearly allowed distinguishing between classical CLL on the one hand, and atypical CLL and MCL on the other hand. The percentage of CD54-positive cells and the median fluorescence intensity of CD20 and CD54 were the only parameters which were significantly higher in MCL than in atypical CLL (P Ͻ 0.05), allowing an immunological distinction between these two entities. Nevertheless, due to a quenching problem when using CD20 and CD54 together, and because CD18 showed a statistically different expression between classical and atypical CLL, the combination of CD18/CD54 has been preferred and showed a different pattern in the three entities. Immunophenotyping could be helpful in the differential diagnosis of CD5-positive B cell chronic lymphoproliferative disorders with atypical features that do not fit exactly into any of the morphologic proposed groups. Leukemia (2001) 15, 1458-1465.

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Section: Table 4bmentioning

confidence: 99%

Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

et al. 2001

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“…The regulation and function of adhesion molecule expression in B-CLL is likely to play an important role in the anatomical distribution and progression of the disease [13,14], Expression of LFA-1 is typically low or absent [14][15][16]. This may be a significant factor in determining the pattern of dissemination of the disease, and may also confer resistance against anti-tumor cell-mediated cyto toxicity.…”

Section: Discussionmentioning

confidence: 99%

Regulation and Function of Adhesion Molecules in B-Cell Chronic Lymphocytic Leukaemia

Jewell

1997

Acta Haematol

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Cell surface expression of adhesion molecules in B-cell chronic lymphocytic leukemia (B-CLL) may determine the patterns of dissemination and infiltration. B-CLL cells express high levels of CD44, but expression of the leucocyte integrins was low or absent, while expression of VLA-4 is high. Most cases examined expressed no detectable ICAM-1, but some cases demonstrated levels of up to 30%. Levels of L-selectin were also variable, and expression could be induced/enhanced in vitro by incubation with cytokines such as IL-4, interferon-α and interferon-γ. B-CLL cells bound normally to vascular endothelium, but binding to IL-1-activated endothelium was significantly lower than that of normal peripheral blood lymphocytes. Cytokine enhancement of L-selectin expression was not accompanied by changes in binding to vascular endothelium. Patterns of adhesion molecule expression and their regulation by cytokines may underly some of the clinical features of this disease.

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“…The strength of surface antigens expression were classified as follows: <20 FI units (negative); 20–100 FI units (weak); 100–450 FI units (moderate); and >450 FI units (strong). Typical CLL phenotype was defined as CD19 + , CD5 + , CD20 + , CD23 + , CD22 + , CD10 − and weak expression of clonal surface immunoglobulin ( Harris et al , 1994 ), CD11a − ( Woessner et al , 1994 ), FMC7 − and CD38 − .…”

Section: Methodsmentioning

confidence: 99%

Trisomy 12 is seen within a specific subtype of B‐cell chronic lymphoproliferative disease affecting the peripheral blood/bone marrow and co‐segregates with elevated expression of CD11a

et al. 1998

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In order to delineate the specific morphological and immunophenotypic features of B-cell lymphoproliferative disorders associated with trisomy 12, 172 sequential unselected cases of CD19 + CD5 + B-cell disorders, primarily affecting the peripheral blood and bone marrow, were studied. Trisomy 12 was found in 24 cases (13.9%), with all cases morphologically classified as either CLL-PL or CLLmixed by FAB criteria. Trisomy 12 was not found in any cases of typical CLL. Trisomy 12 cases demonstrated a significant higher expression of CD11a (P<0.0001) and CD20 (P<0.0006) when compared to cases with the equivalent morphology and immunophenotype, but without the chromosomal abnormality. Trisomy 12 cases also demonstrated a higher frequency of FMC7, CD38 expression and moderate to strong surface immunoglobulin staining. However, no correlation was detected between the percentages of trisomy 12 cells and cells expressing CD11a, CD38, FMC7 or sIg mean fluorescent intensity. Cells from trisomy 12 positive cases were sorted according to their CD11a expression using fluorescent activated cell sorting. There was a significant increase in the percentage of trisomy 12 cells within the CD11a + sorted fraction compared to the unsorted population (P<0.05), implying that trisomy 12 is associated with increased expression of CD11a.With the highly specific morphological and immunophenotypic features demonstrated by trisomy 12 cases in this study, it is highly likely that these cases constitute a specific group of B-cell lymphoproliferative disorders.

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Expression of Lymphocyte Function-Associated Antigen (LFA)-1 in B-Cell Chronic Lymphocytic Leukemia

Cited by 15 publications

References 15 publications

Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

Regulation and Function of Adhesion Molecules in B-Cell Chronic Lymphocytic Leukaemia

Trisomy 12 is seen within a specific subtype of B‐cell chronic lymphoproliferative disease affecting the peripheral blood/bone marrow and co‐segregates with elevated expression of CD11a

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