Expression of leptin receptor in the oviduct of Chinese brown frog (Rana dybowskii)

Abstract

The oviduct of Chinese brown frog () expands specifically during prehibernation instead of in the breeding period. In this study, we investigated the expression of leptin receptor (Ob-Rb) in oviduct during the breeding period and prehibernation. Histologically, the oviduct of consists of glandular cells, tubule lumen, and epithelial cells. The oviductal weight and pipe diameter also revealed significant differences, which were higher in prehibernation than that of the breeding period. Ob-Rb was observed in stromal cells of oviductal tissue in both the breeding period and prehibernation. The mean protein and mRNA levels of the Ob-Rb were significantly higher in prehibernation as compared with the breeding period. In addition, oviductal content of leptin was also higher in prehibernation than that of the breeding period. These results suggested that oviduct of might be a target organ of leptin, and leptin may play an autocrine/paracrine role mediated by Ob-Rb in regulating the oviductal hypertrophy during prehibernation.

Walnut Polyphenol Extract Protects against Fenitrothion-Induced Immunotoxicity in Murine Splenic Lymphocytes

“…Total cell extracts were prepared as previously described [29]. In brief, additional sets of cells were harvested and washed with PBS.…”

17Beta-Estradiol Regulates NUCB2/ Nesfatin-1 Expression in Mouse Oviduct

“…Leptin is also produced in the porcine oviduct for maturation of oocytes and preimplantation embryo development (Craig et al, 2005). Recent report showed that lepitn synthesized and secreted in the oviduct of brown frogs play an important role in autocrine or paracrine regulation in oviductal hypertrophy (Xi et al, 2017). Given these reposts, nesfatin-1 may also play an important role for sperm capacitation and early embryo development in the oviduct like other appetite-regulating hormones.…”