Expression of Leptin, Leptin Receptor, Adiponectin, and Adiponectin Receptor in Ductal CarcinomaIn Situand Invasive Breast Cancer

Abstract

PurposeAdipocytokines, such as leptin, resistin, and adiponectin, are associated with obesity and breast cancer. Several studies have indicated that adipocytokines may influence tumor growth or differentiation. The aims of this study were to determine the expression of leptin, leptin receptor (ObR), adiponectin and adiponectin receptor (AdipoR) in human breast cancer, to evaluate their prognostic significance in the breast cancer.MethodsSpecimens from 198 patients with primary breast cancer were enrolled, and representative paraffin tumor blocks were selected for constructing tissue microarrarys (TMA). Immunohistochemical staining for leptin, ObR, adiponectin, and AdipoR was performed using TMA, and the clinicopathologic characteristics were evaluated from the patient's medical records.ResultsStage 0 breast cancer accounted for 41 cases, and 157 cases were invasive cancer. Positive rates of leptin and ObR expression in the ductal carcinoma in situ (DCIS) group were significantly higher than those of the invasive cancer group (97.4% vs. 34.0%, p<0.001; 74.4% vs. 29.8%, p<0.001). However, positive rates of adiponectin and AdipoR expression in the invasive cancer group were significantly higher than those in the DCIS group (53.7% vs. 33.3%, p=0.024; 59.9% vs. 26.3%, p<0.001). High leptin expression was significantly associated with high Ki-67 expression (p=0.016). High adiponectin expression was significantly correlated with smaller tumor size (p=0.001).ConclusionWe suggest that losses of leptin and ObR expression could be associated with invasive cancer, whereas high adiponectin and AdipoR expression may be associated with breast cancer invasiveness.

Quantification of Steroid Receptors Gene Expression in Breast Cancer Patients: Possible Correlation with Serum Level of Adipocytokines

“…A correlation between adiponectin and angiogenic adipokines suggests that adiponectin may be an effective suppressor of pathological neovascularization [16]. Many studies have shown that the clinical effects and biological actions of adiponectin are opposed of leptin [20]. Studies confirm a significant inverse correlation between serum adiponectin levels and poor-prognosis breast cancer [21].…”

Serum levels of leptin receptor in patients with malignant melanoma as a new tumor marker

“…Epidemiological studies have indicated that obesity is associated with a higher risk for certain cancers caused by elevated levels of adipocyte-derived hormones. It has been reported that leptin is overexpressed in ovarian cancer (2) and breast cancer (3,4). On the other hand, leptin inhibits hepatocellular carcinoma proliferation (1,5).…”

Association between lysyl oxidase and fibrotic focus in relation with inflammation in breast cancer

“…Representative paraffin blocks of invasive breast carcinomas were selected to prepare for tissue microarray (TMA) and TMA was constructed following the methods described in our previous study (20). TMA blocks were cut into 5 µm-thick sections and immunohistochemical staining was performed on the TMA sections using the Bond Polymer Intense Detection System (Leica Microsystems, Victoria, Australia) according to the manufacturer's instruction with minor modifications.…”