Expression of Leptin and Its Receptor in the Murine Ovary: Possible Role in the Regulation of Oocyte Maturation1

Ryan, Natalie K.; Woodhouse, Carole M.; Hoek, Kylie H. Van der; Gilchrist, Robert B.; Armstrong, David T.; Norman, Robert J.

doi:10.1095/biolreprod66.5.1548

Cited by 116 publications

(101 citation statements)

References 34 publications

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“…Leptin receptor mRNA and protein is expressed in mouse oocytes [7,8] and leptin induces tyrosine phosphorylation of STAT3, a major intracellular leptin signal transduction protein, in mouse metaphase 2 stage oocytes [8]. Ryan and co-workers [7] reported that leptin enhances resumption of meiosis in preovulatory follicle-enclosed oocytes in culture, but does not alter meiotic resumption in denuded or cumulus-enclosed oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Ryan and co-workers [7] reported that leptin enhances resumption of meiosis in preovulatory follicle-enclosed oocytes in culture, but does not alter meiotic resumption in denuded or cumulus-enclosed oocytes. Leptin receptor is expressed in mouse 1-cell and 2-cell embryos as well as morulae and blastocysts [9].…”

Section: Discussionmentioning

confidence: 99%

“…Leptin impacts reproduction through a variety of regulatory mechanisms that include the onset of puberty, control of gonadotropin secretion, gonadal function, implantation, and pregnancy. In the ovary, leptin receptor mRNA and protein are expressed in granulosa, theca, luteal, and microvascular endothelial cells [4][5][6][7], as well as oocytes [7,8]. Leptin receptors are also present in early cleavagestage embryos and blastocysts [9].…”

Section: Introductionmentioning

confidence: 99%

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Declining fertility in the lethal yellow mouse is related to progressive hyperleptinemia and leptin resistance

2005

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-Mice possessing the lethal yellow mutation (C57BL/6J A y /a) become obese and develop hyperleptinemia and leptin resistance as they age. To determine the relationship between altered leptin physiology and reproductive function in these mice, we compared body weight (BW), serum leptin concentration, ovulation rate, and in vitro blastocyst development among 120-and 180-d-old lethal yellow and black non-mutant (a/a) mice. Estrous female yellow and black mice were mated with fertile black males. Oviducts were flushed ~ 36 h after mating and the recovered embryos were cultured for 96 h. BW, serum leptin levels, and the leptin:BW ratio differed among groups as follows: 180-d yellow > 120-d yellow > 180-d black = 120-d black. Ovulation rate was similar among 120-d yellow and black, and 180-d black mice. Among 180-d yellow mice, five of twelve mice failed to ovulate, but the other seven mice ovulated a similar number of oocytes as their black counterparts (8.4 ± 0.9 versus 8.0 ± 1.3). Non-ovulators had higher (P < 0.05) leptin levels (56.6 ± 1.8 ng·mL -1 ) than ovulators (46.2 ± 3.5), but BW did not differ significantly. Fewer embryos from 180-d yellow mice reached the blastocyst stage in culture than did the embryos from black mice (55% versus 83%, P < 0.05). Moreover, blastocyst development in 180-d old yellow mice negatively correlated with leptin levels (r = -0.797, P = 0.032) and leptin:BW ratio (r = -0.847, P = 0.016), but not with BW. Declining reproductive function in lethal yellow mice appears to be related to increasing levels of leptin and progression of leptin resistance. obesity / leptin / agouti / reproduction

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Declining fertility in the lethal yellow mouse is related to progressive hyperleptinemia and leptin resistance

2005

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“…While leptin correlates with progesterone concentrations throughout the cycle [61], LEPRs in oocytes might also influence oocyte maturation and development [62]. In fact, in mouse oocytes [63][64][65] leptin induces tyrosine phosphorylation of STAT3, a major intracellular leptin signal transduction protein in mouse metaphase II stage oocytes [64]. Moreover, LEPR and leptin mRNA is specifically expressed at the blastocyst stage, suggesting a function in the blastocyst-endometrial dialog [8].…”

Section: Role Of Leptin In Preinplantational Embryomentioning

confidence: 99%

Role of leptin in female reproduction

Pérez-Pérez

Sánchez-Jiménez

Maymó

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

125

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Abstract:Reproductive function is dependent on energy resources. The role of weight, body composition, fat distribution and the effect of diet have been largely investigated in experimental female animals as well as in women. Any alteration in diet and/or weight may induce abnormalities in timing of sexual maturation and fertility. However, the cellular mechanisms involved in the fine coordination of energy balance and reproduction are largely unknown. The brain and hypothalamic structures receive endocrine and/or metabolic signals providing information on the nutritional status and the degree of fat stores. Adipose tissue acts both as a store of energy and as an active endocrine organ, secreting a large number of biologically important molecules termed adipokines. Adipokines have been shown to be involved in regulation of the reproductive functions. The first adipokine described was leptin. Extensive research over the last 10 years has shown that leptin is not only an adipose tissue-derived messenger of the amount of energy stores to the brain, but also a crucial hormone/cytokine for a number of diverse physiological processes, such as inflammation, angiogenesis, hematopoiesis, immune function, and most importantly, reproduction. Leptin plays an integral role in the normal physiology of the reproductive system with complex interactions at all levels of the hypothalamic-pituitary gonadal (HPG) axis. In addition, leptin is also produced by placenta, where it plays an important autocrine function. Observational studies have demonstrated that states of leptin excess, deficiency, or resistance can be associated with abnormal reproductive function. This review focuses on the leptin action in female reproduction.

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“…The ovary is also believed to be a site of leptin synthesis. The presence of leptin mRNA and protein was observed in human GC and theca cells, follicular fluid, oocytes and CL Cioffi et al, 1997;Loffler et al, 2001;Welt et al, 2003), in murine GC and theca cells, oocytes, CL and OS Ryan et al, 2002 andArchanco et al, 2003) and in porcine CL and OS (Smolinska et al, 2010). The expression of leptin and OB-Rb in porcine ovarian follicles during the cycle and in early pregnancy (implantation) has scarcely been investigated to date.…”

Section: Introductionmentioning

confidence: 99%

Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs

Smolińska

Kamiński

Siawrys

et al. 2013

Animal

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Leptin is a polypeptide hormone produced primarily by adipocytes. It has been implicated in the regulation of satiety and energy homeostasis. Leptin has been suggested to play a role in reproduction based on its involvement in the regulation of the hypothalamic-pituitary-gonadal axis via endocrine, paracrine and/or autocrine pathways. The aim of the present study was to localize the cellular distribution of leptin and the long isoform of leptin receptor (OB-Rb) genes in porcine ovarian antral follicles and to compare the expression levels of leptin and OB-Rb mRNAs in porcine granulosa cells (GC), theca interna (TIC) and theca externa (TEC) cells during the luteal phase of the estrous cycle and in early pregnancy. The expression of leptin and OB-Rb genes was detected in GC, TIC and TEC. Significantly higher levels of leptin gene expression in GC were observed during the mid-and late-luteal phases of the cycle than on days 30 to 32 of pregnancy. On days 14 to 16 of pregnancy, leptin mRNA expression was higher than that on days 14 to 16 of the cycle. The expression of the OB-Rb gene in GC and TEC increased during pregnancy in comparison with the analyzed luteal phases of the cycle. Our results validate the hypothesis that locally produced leptin plays a role in the regulation of porcine reproduction at the ovarian level and exerts a direct effect on porcine follicles. The differences in OB-Rb gene expression in porcine GC and theca cells also suggest that their sensitivity to leptin varies in the ovaries of pregnant and cyclic pigs.

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Expression of Leptin and Its Receptor in the Murine Ovary: Possible Role in the Regulation of Oocyte Maturation1

Cited by 116 publications

References 34 publications

Declining fertility in the lethal yellow mouse is related to progressive hyperleptinemia and leptin resistance

Declining fertility in the lethal yellow mouse is related to progressive hyperleptinemia and leptin resistance

Role of leptin in female reproduction

Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs

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