Expression of laminin-5 and integrins in actinic cheilitis and superficially invasive squamous cell carcinomas of the lip

da-Silva, Janaína Peixoto; Lourenço, Sílvia Vanessa; Nico, Marcello Menta Simonsen; Silva, Filomena H.; Trierveiler, Marília; Costa-Neves, Adriana

doi:10.1016/j.prp.2012.07.004

Cited by 11 publications

(6 citation statements)

References 37 publications

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“…Integrins mediate these events in neoplasms. The more invasive cells have increased expression of integrins, 20 enhancing cell motility, with the participation of many molecules, including extracellular matrix proteins and growth factors such as epidermal growth factor (EGF). 21 In the present study, we observed that after the analysis carried out by MCODE software, cluster 1 interaction network is associated directly with the molecular signals induced by integrins, demonstrating a possible malignant potential for actinic cheilitis injury which may lead to lip cancer ( Figure 3A, Table 1).…”

Section: Resultsmentioning

confidence: 99%

Toxicological Effects of Tobacco Compounds on the Expression of Genes Involved in Actinic Cheilitis

Leal¹,

Santos²,

Duarte³

et al. 2018

Dent Open J.

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Background The molecular effects of substances present in tobacco and cancer development have been well described. Morphological studies have demonstrated tissue changes in patients with these lesions and tobacco substances. However, the effects of the tobacco components and the development of potentially malignant lesions remain unknown. Materials and Methods Thus, by chemical-biological analysis, we investigated compounds present in tobacco and the expression of genes involved in the etiopathogenesis of actinic cheilitis. Results and Discussion Analysis showed a ratio of 51 harmful substances present in tobacco that are involved in several biological processes that can cause abnormal epithelial-mesenchymal transition pathway. Also, describe how cadmium can adversely affect signaling and cell differentiation through the inhibition of specific proteins. Conclusion This study provides the first approach that describes how different tobacco constituents affect a vast network of biological processes in the development of actinic cheilitis and possible progression of the lip carcinoma.

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Section: Resultsmentioning

confidence: 99%

Toxicological Effects of Tobacco Compounds on the Expression of Genes Involved in Actinic Cheilitis

Leal¹,

Santos²,

Duarte³

et al. 2018

Dent Open J.

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“…Our findings may support IHC data for the laminin-5 γ2 chain from other studies, which suggested laminin overexpression during the progression of neoplastic disease in invasive oral cancer [ 34 , 37 ]. Peixoto da-Silva et al [ 46 ] reported negative cytoplasmic staining of the laminin-5 γ2 chain in AC cases, and they also found a cytoplasmic accumulation of laminin-5 in invading cancer cells. Cytoplasmic expression of the γ2 chain of laminin has been previously observed in various types of malignancies, and Peixoto da-Silva et al suggested it may be due to laminin proteolysis products during neoplastic transformation [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Laminin Immunostaining in Biopsies as a Useful Biomarker of Early Invasion in Actinic Cheilitis and Differential Diagnosis Between Actinic Cheilitis and Lip Cancer: New Insights

Vageli

Doukas²,

Zacharouli

et al. 2022

Head and Neck Pathol

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Background Squamous cell carcinoma of the lip (LSCC) and oral cavity can be life-threatening if not diagnosed early. Precancerous lesions like actinic cheilitis (AC), can transform into LSCC. Laminin is a fundamental component for basement membrane (BM) and its integrity may prevent neoplastic invasion. Therefore, laminin immunostaining of BM may be useful in identifying early invasion in actinic cheilitis and thus in the differential diagnosis between AC and invasive LSCC or high-grade epithelial dysplasia (ED). Materials and methods Biopsies from 46 patients with oral lesions were histologically analyzed and immunohistochemically stained for laminin-1. Results AC was diagnosed in 34 patients and LSCC in 12 patients, including 3 patients with AC and concomitant high-grade ED/in situ carcinoma. Laminin-1 immunostaining revealed intense and linear expression of the BM in AC with low-grade ED. Loss of laminin expression was observed in LSCC. Intracellular laminin expression in parabasal cells was noted in AC with high-grade ED/in situ carcinoma. Conclusion Laminin immunostaining could be useful in identifying AC cases suspected of early invasion. It could also contribute to the histopathological differential diagnosis between AC with low- and high-grade ED and between AC and invasive LSCC. The findings of this study provide new insights into the mechanism involved in the progression process of AC into LSCC, encouraging preclinical studies that may document the stochastic role of laminin in this process.

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“…The third gene, lama5, encodes one of the vertebrate laminin alpha chain proteins, a family of extracellular matrix glycoproteins, which are the major noncollagenous constituent of basement membranes. In humans, lama5 has been implicated in pathologenesis of lip in ammation and carcinogenesis [71]. In mouse, loss of lama5 causes hyper-proliferation of basal keratinocytes, an increase in the number of immune cells and thickening of epidermis; thus, reduced expression of lama5 in the hypertophic lip regions might result in an increased number of keratinocytes and subsequently thicker epidermis in these regions [72].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics Unravels Molecular Players Shaping Dorsal Lip Hypertrophy in the Vacuum Cleaner Cichlid, Gnathochromis Permaxillaris

Lecaudey

Singh

Sturmbauer

et al. 2021

Preprint

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Background:Teleosts display a spectacular diversity of craniofacial adaptations that often mediate ecological specializations. A considerable amount of research has revealed molecular players underlying skeletal craniofacial morphologies, but less is known about soft craniofacial phenotypes. Here we focus on a bizarre example of lip hypertrophy in the Lake Tangnayika cichlid, Gnathochromis permaxillaris, that is considered to be a dietary adaptation to suck invertebrates out of narrow crevices. In this study, we investigate the molecular and regulatory basis of lip development G. permaxillaris, using a comparative transcriptomic approach.Results: We identified a gene regulatory network involved in tissue overgrowth and cellular hypertrophy, potentially associated with the formation of a locally restricted hypertrophic lip in a teleost fish species. Of particular interest were the increased expression level of apoda and fhl2, as well as reduced expression of cyp1a, gimap8, lama5 and rasal3, in the hypertrophic lip region which have been implicated in formation of lip structure in other vertebrates. Among the predicted upstream transcription factors, we found reduced expression foxp1 in the hypertrophic lip region, which is known to act as repressor of cell growth and proliferation and its function has been associated with hypertrophy of upper lip in human. Conclusion: Our results provide a genetic foundation for future studies of molecular players shaping soft and exaggerated, but locally restricted, craniofacial morphological changes in fish and perhaps across vertebrates.

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Expression of laminin-5 and integrins in actinic cheilitis and superficially invasive squamous cell carcinomas of the lip

Cited by 11 publications

References 37 publications

Toxicological Effects of Tobacco Compounds on the Expression of Genes Involved in Actinic Cheilitis

Toxicological Effects of Tobacco Compounds on the Expression of Genes Involved in Actinic Cheilitis

Laminin Immunostaining in Biopsies as a Useful Biomarker of Early Invasion in Actinic Cheilitis and Differential Diagnosis Between Actinic Cheilitis and Lip Cancer: New Insights

Transcriptomics Unravels Molecular Players Shaping Dorsal Lip Hypertrophy in the Vacuum Cleaner Cichlid, Gnathochromis Permaxillaris

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