Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions

Luo, Yan; Fukuhara, Masayuki; Weitzman, Matthew D.; Rizzolo, Lawrence J.

doi:10.1016/j.brainres.2006.06.059

Cited by 31 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…29 In our work, we identified an overexpression of PAR-3 associated with an alteration of the localization of the protein both in the R-180 cell line and in the original tumor. Indeed, while PAR-3 was localized at the apical border of the cells in the proximal tubule of healthy kidney, as previously reported for epithelial cells, 30 PAR3 was observed as continuous and diffuse membranous staining and/or cytoplasmic in ccRCC. In addition, PAR-3 The relationship between progression free survival (PFS) and overall survival (OS) rates and low PAR-3 expression (type 1, blue line) or high PAR-3 expression (type 2, red dashed line) was investigated using the Kaplan-Meier statistical method; p < 0.0001 in both cases.…”

Section: Discussionsupporting

confidence: 83%

Overexpression of the polarity protein PAR‐3 in clear cell renal cell carcinoma is associated with poor prognosis

Dugay

Goff²,

Rioux-Leclerq

et al. 2013

Intl Journal of Cancer

View full text Add to dashboard Cite

The partition-defective 3 (PAR-3) protein is implicated in the development and maintenance of cell polarity and is associated with proteins that mediate the changes in cytoskeleton organization required for cell polarity establishment. In this work, we used two original primary cell lines (R-180 and R-305) derived from clear cell Renal Cell Carcinoma (ccRCC) surgical specimens of a patient with unfavorable clinical course (R-180 cells) and a patient with favorable prognosis (R-305 cells) to identify genetic and molecular features that may explain the survival difference of the two patients. The cytogenetic analysis of these cell lines revealed that the PARD3 gene was amplified only in the R-180 cell line that was derived from an aggressive ccRCC. PARD3 gene amplification was associated with overexpression of the encoded protein and altered cytoskeleton organization. Consistently, PARD3 knockdown in R-180 cells restored the cytoskeleton organization and reduced cell migration in comparison to non-transfected cells. Immunohistochemical analysis of ccRCC samples from a cohort of 96 patients with a follow-up of 6 years revealed that PAR-3 overexpression was correlated with poor survival. Our results suggest that PAR-3 has a role in the clinical aggressiveness of ccRCC, possibly by promoting cell migration.Renal cell carcinoma (RCC), the incidence of which is steadily increasing, represents approximately 3.8% of adult malignancies and 90-95% of kidney neoplasms. 1 The most common histological RCC subtype is the conventional or clear cell carcinoma (ccRCC), which accounts for 70-75% of cases. 2,3 At least 60% of ccRCCs have deletions or translocations involving the short arm of chromosome 3, which contains the von Hippel-Lindau (VHL) gene at 3p25. 4,5

show abstract

Section: Discussionsupporting

confidence: 83%

Overexpression of the polarity protein PAR‐3 in clear cell renal cell carcinoma is associated with poor prognosis

Dugay

Goff²,

Rioux-Leclerq

et al. 2013

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Af-6 acts cooperatively with Par3 and it transiently interacts with ZO-1 to localize JAMs and claudins in assembly of TJ and AdJ complexes. 37,49 Scrib1 is indicated to promote basolateral membrane identity and is identified at the transcript level in endothelial cells although its function in brain endothelial cells is still not established. 7 Angiomotin belongs to the motin family of angiostatin binding proteins.…”

Section: Blood-brain Barrier Junctional Complexmentioning

confidence: 99%

“…Af-6, on the other side, participates in regulating cell-cell adhesion, cell polarization, migration, survival and differentiation, acting at the interface between TJ and AdJ complexes. 37,49 Af-6 directly binds to ZO-1, JAM-A and gap junction proteins as well actin-and cadherin-binding proteins. Af-6 acts cooperatively with Par3 and it transiently interacts with ZO-1 to localize JAMs and claudins in assembly of TJ and AdJ complexes.…”

Section: Blood-brain Barrier Junctional Complexmentioning

confidence: 99%

Junctional proteins of the blood-brain barrier: New insights into function and dysfunction

et al. 2016

View full text Add to dashboard Cite

“…Other studies have proposed that JAM-A may primarily function as a regulator of polarity, playing a critical role in assembly and remodeling of retinal pigment epithelial tight junctions. 34 Interestingly, this function has been extended to polymorphonuclear leukocytes (PMNs) during inflammation. 35,36 In this study JAM-A-deficient neutrophils were found to be defective in terms of their polarized movement.…”

Section: Classical Jam Proteinsmentioning

confidence: 99%

JAM Family and Related Proteins in Leukocyte Migration (Vestweber Series)

Bradfield

Nourshargh

Aurrand-Lions

et al. 2007

ATVB

View full text Add to dashboard Cite

Abstract-Exploring the role of junctional adhesion molecules (JAMs) has proven to be varied and controversial. The purpose of this review is to discuss the new and exciting roles of these IgSF molecules and how they have evolved to contribute to diverse functions from development to inflammation. In particular, recent research has focused on JAM subfamily members JAM-A, -B, and -C with newly described roles in leukocyte trafficking during inflammation and angiogenesis. However, research on all JAM family members has demonstrated recurring themes with striking similarities in the many diverse processes they are now known to regulate. (Arterioscler Thromb Vasc Biol. 2007;27:2104-2112.)

show abstract

Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions

Cited by 31 publications

References 35 publications

Overexpression of the polarity protein PAR‐3 in clear cell renal cell carcinoma is associated with poor prognosis

Overexpression of the polarity protein PAR‐3 in clear cell renal cell carcinoma is associated with poor prognosis

Junctional proteins of the blood-brain barrier: New insights into function and dysfunction

JAM Family and Related Proteins in Leukocyte Migration (Vestweber Series)

Contact Info

Product

Resources

About