Expression of Islet1 marks the sensory and neuronal lineages in the mammalian inner ear

Radde-Gallwitz, Kristen; Ling, Pan; Gan, Lin; Lin, Xiaobo; Segil, Neil; Chen, Ping

doi:10.1002/cne.20257

Cited by 120 publications

(140 citation statements)

References 56 publications

(61 reference statements)

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“…Consistent with Math1 (Chen et al, 2002) and Hes6 (Fig. 1) in the vestibule at E12.5, and Islet 1 (Radde-Gallwitz et al, 2004) expression in the cochlea and vestibule at E12.5, messages for the three genes were detected in E12.5 inner ear epithelial tissue (Fig. 5, lane 1).…”

Section: Hes6 Expression In Sensory Hair Cells Depends On Math1supporting

confidence: 71%

“…1A, marked by dotted outline), in addition to its expression in the vestibular epithelium (Fig. 1A) (Radde-Gallwitz et al, 2004). However, Hes6 expression is absent or below the detection limit in the cochleovestibular neurons (Fig.…”

Section: Hes6 Expression Marks the Sensory Lineage In The Vestibulementioning

confidence: 90%

“…At E14.5, the precursor domain of the organ of Corti is marked by the expression of a cyclindependent kinase inhibitor, p27 Kip1 (Cdkn1b) (Chen and Segil, 1999; Fig. 2A), Sox2 (Kiernan et al, 2005a), and Islet1 (Radde-Gallwitz et al, 2004). We and others have observed the onset of Math1 in columns of cells spanning the depth of the cochlear epithelium within the primordial organ of Corti (Chen et al, 2002;Kiernan et al, 2005a;Wang et al, 2005;Fig. 2B,C), and we have hypothesized that these columns of cells are the early hair cell precursors in a multi-cell layered primordial organ of Corti at E14.5.…”

Section: Hes6 Expression Delineates the Morphogenesis Of The Hair Celmentioning

confidence: 99%

“…4C,F). Isl1 is expressed in the otocyst as early as E10.5 and is gradually restricted to the floor of the cochlear duct and to the primordial organ of Corti in the cochlear epithelium (Radde-Gallwitz et al, 2004). Isl1 is also expressed in the neuronal lineage in the inner ear (Radde-Gallwitz et al, 2004).…”

Section: Hes6 Expression In Sensory Hair Cells Depends On Math1mentioning

confidence: 99%

“…The molecular mechanisms underlying the development of the sensory lineage and the patterning of the or-gan of Corti have been studied extensively (Adam et al, 1998;Haddon et al, 1998;Lewis et al, 1998;Lewis, 1998;Bermingham et al, 1999;Lanford et al, 1999Lanford et al, , 2000Morrison et al, 1999;Riley et al, 1999;Eddison et al, 2000;Zhang et al, 2000;Zine et al, 2000Zine et al, , 2001Tsai et al, 2001;Bryant et al, 2002;Chen et al, 2002;Fekete and Wu, 2002;Radde-Gallwitz et al, 2004;Woods et al, 2004;Daudet and Lewis, 2005;Kiernan et al, 2005a,b;Matei et al, 2005). In particular, two classes of basic helix-loop-helix (bHLH)-containing factors, namely the positively and negatively acting transcription factors, have been shown to play essential roles in the differentiation of sensory hair cells and the patterning of the sensory mosaic in the organ of Corti (Bermingham et al, 1999;Zine et al, 2000Zine et al, , 2001Chen et al, 2002;Woods et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Basic helix–loop–helix gene Hes6 delineates the sensory hair cell lineage in the inner ear

et al. 2006

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The basic helix-loop-helix (bHLH) gene Hes6 is known to promote neural differentiation in vitro. Here, we report the expression and functional studies of Hes6 in the inner ear. The expression of Hes6 appears to be parallel to that of Math1 (also known as Atoh1), a bHLH gene necessary and sufficient for hair cell differentiation. Hes6 is expressed initially in the presumptive hair cell precursors in the cochlea. Subsequently, the expression of Hes6 is restricted to morphologically differentiated hair cells. Similarly, the expression of Hes6 in the vestibule is in the hair cell lineage. Hes6 is dispensable for hair cell differentiation, and its expression in inner ear hair cells is abolished in the Math1-null animals. Furthermore, the introduction of Hes6 into the cochlea in vitro is not sufficient to promote sensory or neuronal differentiation. Therefore, Hes6 is downstream of Math1 and its expression in the inner ear delineates the sensory lineage. However, the role of Hes6 in the inner ear remains elusive.

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Section: Hes6 Expression In Sensory Hair Cells Depends On Math1supporting

confidence: 71%

Section: Hes6 Expression Marks the Sensory Lineage In The Vestibulementioning

confidence: 90%

Section: Hes6 Expression Delineates the Morphogenesis Of The Hair Celmentioning

confidence: 99%

Section: Hes6 Expression In Sensory Hair Cells Depends On Math1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Basic helix–loop–helix gene Hes6 delineates the sensory hair cell lineage in the inner ear

et al. 2006

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The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?

2006

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SummaryThe inner ear of mammals uses neurosensory cells derived from the embryonic ear for mechanoelectric transduction of vestibular and auditory stimuli (the hair cells) and conducts this information to the brain via sensory neurons. As with most other neurons of mammals, lost hair cells and sensory neurons are not spontaneously replaced and result instead in age-dependent progressive hearing loss. We review the molecular basis of neurosensory development in the mouse ear to provide a blueprint for possible enhancement of therapeutically useful transformation of stem cells into lost neurosensory cells. We identify several readily available adult sources of stem cells that express, like the ectoderm-derived ear, genes known to be essential for ear development. Use of these stem cells combined with molecular insights into neurosensory cell specification and proliferation regulation of the ear, might allow for neurosensory regeneration of mammalian ears in the near future.

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Mapping of notch activation during cochlear development in mice: Implications for determination of prosensory domain and cell fate diversification

Murata

Tokunaga

Okano

et al. 2006

J of Comparative Neurology

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Recent chick experiments have shown that Notch signaling plays context-dependent distinct roles in inner ear development: initially, Notch activity confers a prosensory character on groups of cells by "lateral induction"; subsequently, it is involved in the establishment of fine-graded patterns of hair cells and supporting cells by "lateral inhibition." However, the spatiotemporal pattern of Notch activation in situ during mammalian inner ear development has not been investigated. In this study, we detected the expression patterns of the activated form of Notch1 (actN1) as well as those of endogenous Notch1, Jagged1 (Jag1), and Math1. ActN1 was detected by immunohistochemistry using an antibody that specifically recognizes the processed form of the intracellular domain of Notch1 cleaved by presenilin/gamma-secretase activity. Between embryonic days (E)12.5 and E14.5, actN1 was weakly detected mainly in the medial region of cochlear epithelium, where Jag1-immunoreactivivty (IR) was also observed. Jag1-IR gradually became stronger in a more sharply defined area, finally becoming localized in supporting cells, while actN1 was detected in an overlapping area. Thus, a positive feedback loop was assumed to exist between the expression of Jag1 and actN1. In addition, actN1 started to be strongly expressed in the cells surrounding Math1-positive hair cell progenitors between E14.5 and E15.5. Strong actN1-IR continued in both a supporting cell lineage and in the greater epithelial ridge during the perinatal stage but ended by P7, suggesting that Notch1 activation may initially demarcate a prosensory region in the cochlear epithelium and then inhibit progenitor cells from becoming hair cells via classical "lateral inhibition."

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Expression of Islet1 marks the sensory and neuronal lineages in the mammalian inner ear

Cited by 120 publications

References 56 publications

Basic helix–loop–helix gene Hes6 delineates the sensory hair cell lineage in the inner ear

Basic helix–loop–helix gene Hes6 delineates the sensory hair cell lineage in the inner ear

The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?

Mapping of notch activation during cochlear development in mice: Implications for determination of prosensory domain and cell fate diversification

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