Expression of Invasiveness of <i>Campylobacter jejuni</i> ssp. <i>jejuni</i> after Serial Intraperitoneal Passages in Mice

Fernández, Heriberto; Vivanco, T.; Eller, G.

doi:10.1046/j.1439-0450.2000.00392.x

Cited by 9 publications

(8 citation statements)

References 18 publications

(27 reference statements)

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“…Both overall invasion efficiency and isolate‐specific variation in invasiveness of C. upsaliensis observed in this study was not untypical of that reported for other enteric campylobacters, including C. jejuni and Campylobacter coli (Konkel et al ., 1992; Schwartz et al ., 1996; Tay et al ., 1996; Biswas et al ., 2000; Fernandez et al ., 2000). It is noteworthy that the ATCC type strain, ATCC43954, showed lower invasiveness on any of the cell lines used in this study.…”

Section: Discussionmentioning

confidence: 99%

“…It is noteworthy that the ATCC type strain, ATCC43954, showed lower invasiveness on any of the cell lines used in this study. That the two clinical isolates were more invasive than ATCC43954 may reflect the extensive in vitro passaging and prolonged storage of the type strain (Konkel et al ., 1990; Babakhani and Joens, 1993; Fernandez et al ., 2000). Although ATCC43954 is the best characterized isolate of C. upsaliensis , it is not typical of other members of this species in a number of ways, other than the degree of invasiveness.…”

Section: Discussionmentioning

confidence: 99%

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Invasion of human epithelial cells byCampylobacter upsaliensis

Mooney

Byrne

Clyne

et al. 2003

Cellular Microbiology

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SummaryFew data exist on the interaction of Campylobacter upsaliensis with host cells, and the potential for this emerging enteropathogen to invade epithelial cells has not been explored. We have characterized the ability of C. upsaliensis to invade both cultured epithelial cell lines and primary human small intestinal cells. Epithelial cell lines of intestinal origin appeared to be more susceptible to invasion than nonintestinal-derived cells. Of three bacterial isolates studied, a human clinical isolate, CU1887, entered cells most efficiently. Although there was a trend towards more efficient invasion of Caco-2 cells by C. upsaliensis CU1887 at lower initial inocula, actual numbers of intracellular organisms increased with increasing multiplicity of infection and with prolonged incubation period. Confocal microscopy revealed C. upsaliensis within primary human small intestinal cells. Both Caco-2 and primary cells in non-confluent areas of the infected monolayers were substantially more susceptible to infection than confluent cells. The specific cytoskeletal inhibitors cytochalasin B, cytochalasin D and vinblastine attenuated invasion of Caco-2 cells in a concentration-dependent manner, providing evidence for both microtubule-and microfilament-dependent uptake of C. upsaliensis . Electron microscopy revealed the presence of organisms within Caco-2 cell cytoplasmic vacuoles. C. upsaliensis is capable of invading epithelial cells and appears to interact with host cell cytoskeletal structures in order to gain entry to the intracellular environment. Entry into cultured primary intestinal cells ex vivo provides strong support for the role of host cell invasion during human enteric C. upsaliensis infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Invasion of human epithelial cells byCampylobacter upsaliensis

Mooney

Byrne

Clyne

et al. 2003

Cellular Microbiology

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“…This latter class of proteins is described in pathogenic Corynebacterium species and may contribute especially in the process of cellular adhesion [48]. In Campylobacter jejuni, serial passages in mice induce the expression of invasiveness and increase the capacity of cell invasion [13]. Components of the Opp system were induced by the passage process, too.…”

Section: Discussionmentioning

confidence: 99%

A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomics

et al. 2017

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Background Corynebacterium pseudotuberculosis biovar ovis, a facultative intracellular pathogen, is the etiologic agent of caseous lymphadenitis in small ruminants. During the infection process, C. pseudotuberculosis changes its gene expression to resist different types of stresses and to evade the immune system of the host. However, factors contributing to the infectious process of this pathogen are still poorly documented. To better understand the C. pseudotuberculosis infection process and to identify potential factors which could be involved in its virulence, experimental infection was carried out in a murine model using the strain 1002_ovis and followed by a comparative proteomic analysis of the strain before and after passage.ResultsThe experimental infection assays revealed that strain 1002_ovis exhibits low virulence potential. However, the strain recovered from the spleen of infected mice and used in a new infection challenge showed a dramatic change in its virulence potential. Label-free proteomic analysis of the culture supernatants of strain 1002_ovis before and after passage in mice revealed that 118 proteins were differentially expressed. The proteome exclusive to the recovered strain contained important virulence factors such as CP40 proteinase and phospholipase D exotoxin, the major virulence factor of C. pseudotuberculosis. Also, the proteome from recovered condition revealed different classes of proteins involved in detoxification processes, pathogenesis and export pathways, indicating the presence of distinct mechanisms that could contribute in the infectious process of this pathogen.ConclusionsThis study shows that C. pseudotuberculosis modifies its proteomic profile in the laboratory versus infection conditions and adapts to the host context during the infection process. The screening proteomic performed us enable identify known virulence factors, as well as potential proteins that could be related to virulence this pathogen. These results enhance our understanding of the factors that might influence in the virulence of C. pseudotuberculosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-0925-6) contains supplementary material, which is available to authorized users.

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“…Similar results from an evaluation of the virulence potential of Shiga toxin (Stx)-producing Escherichia coli (STEC) were also observed when mice were infected with a lower dose of the recovered bacteria that had been recovered after serial passage in a murine model (Fernandez-Brando et al, 2012). Other studies have also shown that the serial passage process through in vitro or in vivo models leads to changes in the virulence potential of pathogens, including Helicobacter pylori, Escherichia coli, Xenorhabdus nematophila, Arcobacter butzleri, Salmonella enterica , and Shigella flexneri (Fernández et al, 2000, 2013; Bleich et al, 2005; Chapuis et al, 2011; Fernandez-Brando et al, 2012; Liu et al, 2015). Changes in the virulence potential of these pathogens, as well as in 258_ equi , show that this strategy promotes the activation of genes related to bacterial pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, several studies have used mice as a model for studying both the pathogenic process (Jolly, 1965; Zaki, 1966; Nieto et al, 2009) and vaccination testing against infection by C. pseudotuberculosis (Simmons et al, 1997; Lan et al, 1999; Gorman et al, 2010; Ribeiro et al, 2014; Droppa-Almeida et al, 2016). In regards to host-bacteria interactions, some work has explored the serial passage process of bacterial pathogens in vitro or in an in vivo model to identify factors that might be involved in virulence (Fernández et al, 2000, 2013; Bleich et al, 2005; Chapuis et al, 2011; Fernandez-Brando et al, 2012; Liu et al, 2015). In this study, we adopted an in vivo assay in which the strain 258_ equi was experimentally inoculated in mice followed by high-throughput proteomic analysis.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host

Silva

Carvalho

Dorella

et al. 2017

Front. Cell. Infect. Microbiol.

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Corynebacterium pseudotuberculosis biovar equi is the etiologic agent of ulcerative lymphangitis. To investigate proteins that could be related to the virulence of this pathogen, we combined an experimental passage process using a murine model and high-throughput proteomics with a mass spectrometry, data-independent acquisition (LC-MSE) approach to identify and quantify the proteins released into the supernatants of strain 258_equi. To our knowledge, this approach allowed characterization of the exoproteome of a C. pseudotuberculosis equi strain for the first time. Interestingly, the recovery of this strain from infected mouse spleens induced a change in its virulence potential, and it became more virulent in a second infection challenge. Proteomic screening performed from culture supernatant of the control and recovered conditions revealed 104 proteins that were differentially expressed between the two conditions. In this context, proteomic analysis of the recovered condition detected the induction of proteins involved in bacterial pathogenesis, mainly related to iron uptake. In addition, KEGG enrichment analysis showed that ABC transporters, bacterial secretion systems and protein export pathways were significantly altered in the recovered condition. These findings show that secretion and secreted proteins are key elements in the virulence and adaptation of C. pseudotuberculosis. Collectively, bacterial pathogenesis-related proteins were identified that contribute to the processes of adherence, intracellular growth and evasion of the immune system. Moreover, this study enhances our understanding of the factors that may influence the pathogenesis of C. pseudotuberculosis.

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Expression of Invasiveness of Campylobacter jejuni ssp. jejuni after Serial Intraperitoneal Passages in Mice

Cited by 9 publications

References 18 publications

Invasion of human epithelial cells byCampylobacter upsaliensis

Invasion of human epithelial cells byCampylobacter upsaliensis

A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomics

Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host

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