Expression of intracellular cytokines, HSP72, and apoptosis in monocyte subsets during exertional heat stress in trained and untrained individuals

Selkirk, Glen A.; McLellan, Tom M.; Wright, Heather E.; Rhind, Shawn G.

doi:10.1152/ajpregu.90683.2008

Cited by 49 publications

(46 citation statements)

References 92 publications

(150 reference statements)

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“…Supporting the absence of eHsp72 increases in TEMP and HOT (T rec of 37.90±0.29°C and 38.35±0.52°C, respectively) in the present study, parallel exercise induced changes in T rec data (mean maximum T rec 38.48°C) resulting in no change in basal eHsp72 have been reported by Hom et al (2012) during treadmill walking. Mechanistically, temperatures >38.5°C at the hepatosplanchnic viscera are perhaps the most important for increase concentration, with eHsp72 release potentially dependent on the magnitude and duration above this element of the "threshold" (Rhind et al 2004;Selkirk et al 2008Selkirk et al , 2009). Recent evidence (Périard et al 2012) suggests that the same eHsp72 expression is yielded by short (27.2 min) and longer duration (58.9 min) trials by increasing exercise intensity (from 60 to 75 % of V : O 2peak ) with similarity in eHsp72 expression despite differences in peak and mean T rec (39.0 and 39.7°C, respectively).…”

Section: Discussionmentioning

confidence: 99%

“…Caution must be exercised when inferences to a critical endogenous criteria model is made across a broad demographic of exercise capacities (i.e. untrained through to highly trained) as such differences are known to influence eHsp72 release kinetics and magnitudes within thermally challenging environments (Selkirk et al 2008(Selkirk et al , 2009. Therefore, future work should tightly control this potentially confounding variable.…”

Section: Discussionmentioning

confidence: 99%

“…The confounding variables of smoking (Anbarasi et al 2006), caffeine (Lu et al 2008), glutamine (Singleton et al 2004), generic supplementation (Hillman et al 2011), thermal exposures (Selkirk et al 2009), hypoxic exposures (Taylor et al 2010a), hyperbaric exposures (Taylor et al 2012) and alcohol (Taylor et al 2010b) were all controlled in line with previous work in the field . Each volunteer was given instructions for dietary requirements in accordance with published guidelines and requested to maintain identical diets in the immediate 48 h prior to each experimental session (Canada 2009).…”

Section: Volunteersmentioning

confidence: 99%

“…Research has identified extracellular changes in Hsp72 concentration within whole blood (Marshall et al 2006;Yamada et al 2007;Ogura et al 2008;Magalhães et al 2010;Périard et al 2012), and intracellular changes in total protein expression and/or gene transcription in monocytes and systemic tissue (McClung et al 2008;Selkirk et al 2009;Magalhães et al 2010;Amorim et al 2011) in response to thermal and exercise stress. Hsp72 binds with high affinity to the plasma membrane (Asea et al 2000) and up-regulates expression of pro-inflammatory cytokines, tumour necrosis factor-α, interleukin-1β and interleukin-6 in human monocytes.…”

Section: Introductionmentioning

confidence: 99%

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Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure

Gibson

Alex

Parfitt

et al. 2014

Cell Stress and Chaperones

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Extracellular heat shock protein 72 (eHsp72) concentration increases during exercise-heat stress when conditions elicit physiological strain. Differences in severity of environmental and exercise stimuli have elicited varied response to stress. The present study aimed to quantify the extent of increased eHsp72 with increased exogenous heat stress, and determine related endogenous markers of strain in an exercise-heat model. Ten males cycled for 90 min at 50 % V : O 2peak in three conditions (TEMP, 20°C/63 % RH; HOT, 30.2°C/51%RH; VHOT, 40.0°C/37%RH). Plasma was analysed for eHsp72 pre, immediately post and 24-h post each trial utilising a commercially available ELISA. Increased eHsp72 concentration was observed post VHOT trial (+172.4 %) (p <0.05), but not TEMP (−1.9 %) or HOT (+25.7 %) conditions. eHsp72 returned to baseline values within 24 h in all conditions. Changes were observed in rectal temperature (T rec ), rate of T rec increase, area under the curve for T rec of 38.5 and 39.0°C, duration T rec ≥38.5 and ≥39.0°C, and change in muscle temperature, between VHOT, and TEMP and HOT, but not between TEMP and HOT. Each condition also elicited significantly increasing physiological strain, described by sweat rate, heart rate, physiological strain index, rating of perceived exertion and thermal sensation.Stepwise multiple regression reported rate of T rec increase and change in T rec to be predictors of increased eHsp72 concentration. Data suggests eHsp72 concentration increases once systemic temperature and sympathetic activity exceeds a minimum endogenous criteria elicited during VHOT conditions and is likely to be modulated by large, rapid changes in core temperature.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Volunteersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure

Gibson

Alex

Parfitt

et al. 2014

Cell Stress and Chaperones

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“…On the experimental days, all subjects arrived at the laboratory at either 6:30 or 7:30 am after an overnight fast. Experimental exercise tests were conducted at room temperature (25°C) to avoid environmental stress which might induce eHSP70, such as temperature changes (Selkirk et al 2009), hypoxia (Taylor et al 2010a), and hyperbaric changes (Taylor et al 2012). All samples were collected between 7:00 and 8:00 am before exercise.…”

Section: Subjectsmentioning

confidence: 99%

Salivary extracellular heat shock protein 70 (eHSP70) levels increase after 59 min of intense exercise and correlate with resting salivary secretory immunoglobulin A (SIgA) levels at rest

Murase

Shimizu

Tanimura

et al. 2016

Cell Stress and Chaperones

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This study aimed to identify the response of a salivary stress protein, extracellular heat shock protein (eHSP70), to intense exercise and to investigate the relationship between salivary eHSP70 and salivary immunoglobulin A (SIgA) levels in response to exercise. Sixteen healthy sedentary young males (means±SD 23.8±1.5 years, 172.2±6.4 cm, 68.3±7.4 kg) performed 59 min of cycling exercise at 75 % VO2 max . Saliva and whole blood samples were collected before (Pre), immediately after (Post), and at 1, 2, 3, and 4 h after completion of the exercise (1, 2, 3, and 4 h). The salivary eHSP70 and SIgA levels were measured by enzyme-linked imunosorbent assay (ELISA), and the secretion rates were computed by multiplying the concentration by the saliva flow rate. White blood cells were analyzed using an automated cell counter with a directcurrent detection system. The salivary eHSP70 secretion rates were 1

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Encapsulated Environment

McLellan

Daanen²,

Cheung

2013

Comprehensive Physiology

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In many occupational settings, clothing must be worn to protect individuals from hazards in their work environment. However, personal protective clothing (PPC) restricts heat exchange with the environment due to high thermal resistance and low water vapor permeability. As a consequence, individuals who wear PPC often work in uncompensable heat stress conditions where body heat storage continues to rise and the risk of heat injury is greatly enhanced. Tolerance time while wearing PPC is influenced by three factors: (i) initial core temperature (Tc), affected by heat acclimation, precooling, hydration, aerobic fitness, circadian rhythm, and menstrual cycle (ii) Tc tolerated at exhaustion, influenced by state of encapsulation, hydration, and aerobic fitness; and (iii) the rate of increase in Tc from beginning to end of the heat-stress exposure, which is dependent on the clothing characteristics, thermal environment, work rate, and individual factors like body composition and economy of movement. Methods to reduce heat strain in PPC include increasing clothing permeability for air, adjusting pacing strategy, including work/rest schedules, physical training, and cooling interventions, although the additional weight and bulk of some personal cooling systems offset their intended advantage. Individuals with low body fatness who perform regular aerobic exercise have tolerance times in PPC that exceed those of their sedentary counterparts by as much as 100% due to lower resting Tc, the higher Tc tolerated at exhaustion and a slower increase in Tc during exercise. However, questions remain about the importance of activity levels, exercise intensity, cold water ingestion, and plasma volume expansion for thermotolerance.

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Expression of intracellular cytokines, HSP72, and apoptosis in monocyte subsets during exertional heat stress in trained and untrained individuals

Cited by 49 publications

References 92 publications

Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure

Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure

Salivary extracellular heat shock protein 70 (eHSP70) levels increase after 59 min of intense exercise and correlate with resting salivary secretory immunoglobulin A (SIgA) levels at rest

Encapsulated Environment

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