Expression of intermediate filament desmin and vimentin in the human fetal heart

Kim, Ho-Dirk

doi:10.1002/(sici)1097-0185(199610)246:2<271::aid-ar13>3.0.co;2-l

Cited by 35 publications

(28 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Vimentin expression has rarely been assayed in neonatal and adult cardiomyocytes in vitro and has been implicated as part of a dedifferentiation or developmental program largely because embryonic myocytes in vivo and in vitro express vimentin during a fetal transition period (12,21,28,29,31,46,52). The present study is the first to directly demonstrate the emergence of coexpression of vimentin and MyHC in a highly purified postnatal cardiomyocyte culture.…”

Section: Discussionmentioning

confidence: 99%

Cardiac fibroblasts influence cardiomyocyte phenotype in vitro

LaFramboise

Scalise²,

Stoodley³

et al. 2007

American Journal of Physiology-Cell Physiology

138

120

View full text Add to dashboard Cite

Cardiac fibroblasts impact myocardial development and remodeling through intercellular contact with cardiomyocytes, but less is known about noncontact, profibrotic signals whereby fibroblasts alter cardiomyocyte behavior. Fibroblasts and cardiomyocytes were harvested from newborn rat ventricles and separated by serial digestion and gradient centrifugation. Cardiomyocytes were cultured in 1) standard medium, 2) standard medium diluted 1:1 with PBS, or 3) standard medium diluted 1:1 with medium conditioned ≥72 h by cardiac fibroblasts. Serum concentrations were held constant under all media conditions, and complete medium exchanges were performed daily. Cardiomyocytes began contracting within 24 h at clonal or mass densities with <5% of cells expressing vimentin. Immunocytochemical analysis revealed progressive expression of α-smooth muscle actin in cardiomyocytes after 24 h in all conditions. Only cardiomyocytes in fibroblast-conditioned medium stopped contracting by 72 h. There was a significant, sustained increase in vimentin expression specific to these cultures (means ± SD: conditioned 46.3 ± 6.0 vs. control 5.3 ± 2.9%, P < 0.00025) typically with cardiac myosin heavy chain coexpression. Proteomics assays revealed 10 cytokines (VEGF, GRO/KC, monocyte chemoattractant protein-1, leptin, macrophage inflammatory protein-1α, IL-6, IL-10, IL-12p70, IL-17, and tumor necrosis factor-α) at or below detection levels in unconditioned medium that were significantly elevated in fibroblast-conditioned medium. Latent transforming growth factor-β and RANTES were present in unconditioned medium but rose to higher levels in conditioned medium. Only granulocyte-macrophage colony-stimulating factor was present above threshold levels in standard medium but decreased with fibroblast conditioning. These data indicated that under the influence of fibroblast-conditioned medium, cardiomyocytes exhibited marked hypertrophy, diminished contractile capacity, and phenotype plasticity distinct from the dedifferentiation program present under standard culture conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Cardiac fibroblasts influence cardiomyocyte phenotype in vitro

LaFramboise

Scalise²,

Stoodley³

et al. 2007

American Journal of Physiology-Cell Physiology

138

120

View full text Add to dashboard Cite

show abstract

“…Vimentin is an intermediate filament protein that is predominantly expressed in cells of mesodermal origin, including fibroblasts, endothelial cells, and white blood cells, and that tends to form an irregular network in endomysial and perimysial sheaths of the myocardium [18,19]. Vimentin increases in association with other cytoskeletal proteins in the myocardium during stressful conditions such as heart failure [20,21].…”

Section: Experimental Designmentioning

confidence: 99%

Effect of bisphenol A on the myocardium of adult male albino rats and the possible role of lycopene

El-Haleem

Abass

2012

The Egyptian Journal of Histology

View full text Add to dashboard Cite

“…Vimentin expression in this study is similar to the expression pattern in the mylohyoid where no significant difference was observed among the groups during development 1) . However, in human fetal skeletal muscle and myocardium, a decrease in vimentin expression was observed 10,11) . Nevertheless, vimentin expressed by the surrounding tissues caused the development of the muscle and bone tissues particularly the MP and mandible.…”

Section: Discussionmentioning

confidence: 95%

“…Moreover, bone tissue development is stimulated by adjacent muscles through mechanical stress. In human fetal skeletal muscle, desmin increases with the growth of the fetus, the myocardium 10,11) . The study clearly showed the strong desmin integration in the part of the future tendons suggesting the site of mechanical stress.…”

Section: Discussionmentioning

confidence: 99%

Medial Pterygoid initiated the Growth of the Mandible through Premature Muscle Contraction

Kikuchi

Yamamoto

Kitamura

et al. 2014

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Craniofacial growth is influenced by the interaction of muscle and bone tissues. The medial pterygoid (MP) is one of the muscles of mastication attached to the mandible. The purpose of the study was to investigate the relation between the MP and mandible during embryogenesis. Specimens were prepared from fifty fetal mice at embryonic day (ED) 12.5, 13.5, 14.5 and 15.5. Slides were stained with hematoxylin and eosin (H&E) and observed under the light microscope. Immunohistochemistry using desmin, a muscle specific marker, as well as vimentin, a mesenchymal specific marker, were also carried out. Desmin and vimentin mRNA expressions were analyzed by quantitative polymerase chain reaction. Results showed that at ED 12.5, the MP and lateral pterygoid (LP) and tensor veli palatine (TVP) were adjacent to one another. Over time, the LP moved more externally while the TVP moved more medially. At ED 13.5, the mandible started to form while the MP moved towards the developing mandible. At ED 14.5, the palatine shelves were also seen in a horizontal position. Over time, desmin localization was observed at myotendinous junctions in between the MP and Meckel's cartilage (MC) as well as in between the MP and mandible and finally in the center of the muscle. The increase in the expression was supported by desmin mRNA expression. Vimentin was widely distributed in mesenchymal cells with no significant difference among the stages. The results suggest that although the muscles of mastication were still immature, the premature contraction of the MP and the positional relationship provide a dynamic change between the development growth of the mandible and the start of the fusion of the secondary palate.

show abstract

Expression of intermediate filament desmin and vimentin in the human fetal heart

Cited by 35 publications

References 23 publications

Cardiac fibroblasts influence cardiomyocyte phenotype in vitro

Cardiac fibroblasts influence cardiomyocyte phenotype in vitro

Effect of bisphenol A on the myocardium of adult male albino rats and the possible role of lycopene

Medial Pterygoid initiated the Growth of the Mandible through Premature Muscle Contraction

Contact Info

Product

Resources

About