Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro

Crowe, Sheila E.; Álvarez, Luis Gonzalo; Dytoc, Marlene; Hunt, Richard H.; Muller, Milan J.; Sherman, Philip M.; Patel, Janak A.; Jin, Yide; Ernst, Peter B.

doi:10.1016/0016-5085(95)90009-8

Cited by 272 publications

(166 citation statements)

References 35 publications

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“…pylori stimulates the gastric epithelium to initiate inflammation and neutrophil recruitment and activation, and chemokines including IL-8 have been proven to be responsible. IL-8 has been documented as a crucial factor for inducing several types of neutrophil-mediated inflammation in gastric epithelial cells (23,37), and in the present study, exposure of gastric epithelial cells to H. pylori induced secretion of the cytokine IL-8 within 4 hr, which was maximal at 16 hr upon coincubation with H. pylori (data not shown). Although RGE alone was ineffective in decreasing H. pylori-induced IL-8 production ( Figure 3A), IL-8 gene expression following H. pylori infection was effectively attenuated by RGE pretreatment as shown in Figure 3B, suggesting that RGE might hinder the adherence of H. pylori to gastric epithelial cells or be a less potent inhibitor of the signaling pathway to H. pyloriinduced changes.…”

Section: Discussionsupporting

confidence: 67%

Rescue of Helicobacter pylori–Induced Cytotoxicity by Red Ginseng

et al. 2005

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Helicobacter pylori has been known to provoke gastric inflammation, ulceration, and DNA damage, based on which WHO defined H. pylori as a class I carcinogen. Although ginseng, the root of Panax ginseng C.A. Meyer, has been reported to possess antiadhesion or antimicrobial activity against H. pylori, in this study, we examined the protective effect of red ginseng extracts (RGE) against H. pylori-induced cytotoxicity and DNA damage. RGE significantly attenuated both H. pyloriinduced DNA damage assessed by comet assay and apoptosis measured by DNA fragmentation. Inactivation of ERK1/2 signaling and attenuation of caspase-3 activation and PARP cleavage were revealed with RGE against H. pylori infection. RGE decreased H. pylori-stimulated IL-8 gene expression, which resulted from the transcriptional regression of NF-κB. In conclusion, RGE showed significant gastroprotective effects against H. pylori-associated gastric mucosal cell damage, suggesting that red ginseng could be used as a medicinal phytonutrient against H. pylori infection.

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Section: Discussionsupporting

confidence: 67%

Rescue of Helicobacter pylori–Induced Cytotoxicity by Red Ginseng

et al. 2005

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“…21 In addition, several studies using in vitro experimental systems have suggested that this attachment accelerates the production of interleukin-8, which induces neutrophil infiltration. 22,23 In the human stomach, as shown by Shimizu et al, 24 this organism actively col-onizes not only the apical surface but also the SMGL. Our findings in Mongolian gerbils seem to support this notion.…”

Section: Discussionmentioning

confidence: 91%

Helicobacter pylori-Induced Chronic Active Gastritis, Intestinal Metaplasia, and Gastric Ulcer in Mongolian Gerbils

Ikeno

Ota

Sugiyama

et al. 1999

The American Journal of Pathology

150

116

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“…Once they arrive in the mucosa, these cells become active and release enzymes that are potentially capable of causing epithelial damage (1). Several lines of evidence indicate that locally induced IL-8 plays a major role in the recruitment of PMN (3)(4)(5)7). Although Hp by itself or its product has been reported to be a potent inducer of IL-8 synthesis, it is well known that cytokines released by activated macrophages and T cells may modulate IL-8 expression in both mononuclear and epithelial cells (9).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years considerable evidence has been accumulated to indicate that IL-8, the major human PMN chemoattractant, plays a major role in the Hp-associated acute inflammatory response (2)(3)(4). Both IL-8 mRNA and protein levels strictly correlate with Hp density (5,6).…”

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confidence: 99%

Up-Regulation of IL-17 Is Associated with Bioactive IL-8 Expression inHelicobacter pylori-Infected Human Gastric Mucosa

Luzza

Parrello

Monteleone

et al. 2000

The Journal of Immunology

245

192

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Helicobacter pylori (Hp)-associated gastritis is characterized by an increased number of acute and chronic inflammatory cells secreting cytokines that contribute to maintain and expand the local inflammation. Locally induced IL-8 is believed to play a major role in the Hp-associated acute inflammatory response. Factors/mechanisms that regulate IL-8 induction are, however, not fully understood. In the present study we investigated whether Hp infection is associated with an increased production of IL-17, a T cell-derived cytokine capable of modulating IL-8 gene expression. We showed that both IL-17 RNA transcripts and protein were expressed at a higher level in the whole gastric mucosal and lamina propria mononuclear cell samples from Hp-infected patients than in those from uninfected subjects. Hp eradication was associated with a marked down-regulation of IL-17 expression. The addition of a neutralizing anti-IL-17 Ab to the gastric lamina propria mononuclear cell cultures resulted in a significant inhibition of IL-8 secretion, indicating that IL-17 contributes to enhance IL-8 in the Hp-colonized gastric mucosa. Consistently, stimulation of MKN 28 cells, a gastric epithelial cell line, with IL-17 increased IL-8 secretion. Finally, conditioned medium from the IL-17-stimulated MKN 28 cell cultures promoted the in vitro polymorphonuclear leukocyte migration. This effect was inhibitable by a neutralizing IL-8 but not IL-17 Ab. Together, these data indicate that biologically active IL-17 production is increased during Hp infection, suggesting the possibility that this cytokine may play an important role in the inflammatory response to the Hp colonization.

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Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro

Cited by 272 publications

References 35 publications

Rescue of Helicobacter pylori–Induced Cytotoxicity by Red Ginseng

Rescue of Helicobacter pylori–Induced Cytotoxicity by Red Ginseng

Helicobacter pylori-Induced Chronic Active Gastritis, Intestinal Metaplasia, and Gastric Ulcer in Mongolian Gerbils

Up-Regulation of IL-17 Is Associated with Bioactive IL-8 Expression inHelicobacter pylori-Infected Human Gastric Mucosa

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