“…In addition, change in the expression of a small handful of genes may not lead to changes in protein expression and immune response. Frenette et al [ 28 ] found that changes in the expression of hundreds of genes only led to a 17-fold change in protein expression, thus more exploratory research possibly with RNA sequencing of the transcriptome is needed to determine the extent of these dietary effects. In addition, analysis of other tissues rather than the liver that are more active in the immune system, such as head kidney and spleen, are also recommended to improve the evaluation of the immune response in future studies.…”