Expression of Interferon-Gamma-Inducible Protein-10 and Its Receptor CXCR3 in Chronic Pancreatitis

Singh, Lipi; Bakshi, Dapinder Kaur; Majumdar, S.; Vasishta, Rakesh Kumar; Arora, Sunil; Wig, J D

doi:10.1159/000108965

Cited by 16 publications

(14 citation statements)

References 40 publications

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“…Active research on pancreatitis has been taking place in our laboratory for the past 10 years [11,19,20]. Recently, the expression of various CXC and CC chemokines, especially Interferon-c inducible protein 10 and its receptor CXCR3, was studied in tissues derived from patients' with chronic pancreatitis [21]. Further, to assess the role of chemokines in apoptosis of acinar cells during CP, in vitro experiments were planned for which we required the primary culture of human pancreatic acinar cells.…”

Section: Discussionmentioning

confidence: 99%

Primary Culture of Pancreatic (Human) Acinar Cells

et al. 2008

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The acinar cell culture plays a very important role in research of pancreatic pathophysiology. The aim of this study was to establish a long-term culture of human (foetal) pancreatic acinar cells in standardized nutrient media with supplements. Acinar cells were prepared from pancreatic tissues obtained from aborted foetus (> or =35 weeks) with no prior pancreatic complications by collagenase digestion and cultured using different media and supplements. The purity and phenotype of acinar cells was confirmed by various staining techniques and FACS. The acinar cell proliferation was determined at different time intervals by Bromo-deoxyuridine (BrdU) incorporation, and metabolic enzyme activity was analysed. The acini could be cultured and maintained in Ham's F-12 K/M199 media in the presence of 5% BSA, 0.1 mg/ml STI, 10 ng/ml EGF, and 10% FCS with the same morphological appearance as that of freshly prepared for 12 days with maximum viability of 80-85% and formation of monolayer without extracellular matrix. A significant BrdU incorporation of acinar cells in primary culture was observed which was maximum (105%) at day four. Higher amylase and lipase activity was seen in freshly isolated acinar cells which decreased with time of the culture. The established human pancreatic acinar cell culture may act as an excellent model to study exocrine dysfunction or pancreatitis in response to acinar cell injury.

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Section: Discussionmentioning

confidence: 99%

Primary Culture of Pancreatic (Human) Acinar Cells

et al. 2008

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“…Previously, it has been reported that the existence of chemokine C-X-C motif ligand 10 (CXCL10) and chemokine C-X-C motif receptor 3 (CXCR3) with other chemokine C-X-C motif/CC chemokine subfamily chemokine signatures in humans with CP is associated with the progression of chronic inflammation (11). Moreover, overexpression of another inflammatory initiator, toll-like receptor 4, has been reported to be upregulated in epithelial (pancreatic duct) or endothelial tissues in cerulein-induced pancreatitis in rats (12).…”

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confidence: 99%

Increased Chromogranin A–Positive Hormone-Negative Cells in Chronic Pancreatitis

Moin

Cory

Choi

et al. 2018

The Journal of Clinical Endocrinology &Amp; Metabolism

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Context: Chronic pancreatitis (CP) is characterized by inflammation, fibrosis, and a loss of pancreatic acinar cells, which can result in exocrine and eventually endocrine deficiency. Pancreatitis has been reported to induce formation of new endocrine cells (neogenesis) in mice. Our recent data have implicated chromogranin A-positive hormone-negative (CPHN) cells as potential evidence of neogenesis in humans.Objective: We sought to establish if CPHN cells were more abundant in CP in humans.Design, Setting, and Participants: We investigated the frequency and distribution of CPHN cells and the expression of the chemokine C-X-C motif ligand 10 (CXCL10) and its receptor chemokine C-X-C motif receptor 3 in pancreas of nondiabetic subjects with CP.Results: CPHN cell frequency in islets was increased sevenfold in CP [2.1% 6 0.67% vs 0.35% 6 0.09% CPHN cells in islets, CP vs nonpancreatitis (NP), P , 0.01], as were the CPHN cells found as scattered cells in the exocrine areas (17.4 6 2.9 vs 4.2 6 0.6, CP vs NP, P , 0.001). Polyhormonal endocrine cells were also increased in CP (2.7 6 1.2 vs 0.1 6 0.04, CP vs NP, % of polyhormonal cells of total endocrine cells, P , 0.01), as was expression of CXCL10 in a and b cells. Conclusion:There is increased islet endogenous expression of the inflammation marker CXCL10 in islets in the setting of nondiabetic CP and an increase in polyhormonal (insulin-glucagon expressing) cells. The increase in CPHN cells in CP, often in a lobular distribution, may indicate foci of attempted endocrine cell regeneration. (J Clin Endocrinol Metab

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“…17 The mean age of the CP patients was 35 ± 10 years with a male to female ratio of 7 : 1, while that of the control donors was 36 ± 6 years, with a male to female ratio of 4 : 1. CP was associated with long history of excessive alcohol consumption in 44% of the patients, was idiopathic in 20%, and of unspecifi ed etiology in 36%.…”

Section: Clinical Data and Histopathological Assessmentmentioning

confidence: 94%

“…The fi nal diagnosis was confi rmed by routine histopathological assessment, and then the pancreatic tissues were histopathologically graded into grades I, II, or III. 17 The study received approval from the Institute Review Board, and informed consent was obtained from all patients as prescribed by the institutional ethics committee in accordance with the institute's guidelines.…”

Section: Subjectsmentioning

confidence: 99%