Expression of inter-alpha-trypsin inhibitor heavy chains in endometrium of cyclic and pregnant gilts

Geisert, R. D.; Ashworth,; Malayer,

doi:10.1530/rep.0.1260621

Cited by 23 publications

(14 citation statements)

References 22 publications

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“…Extrahepatic production of ALB, APOA1, SAA, Hp, and PigMAP has previously been described in pigs [12, 32, 36, 40]. However, the current study provides to our knowledge the first example of extrahepatic expression of AGP, CRP, FIB, Hp, PigMAP, TF, and TTR in porcine peripheral lymphoid tissues.…”

Section: Discussionmentioning

confidence: 52%

Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs

et al. 2009

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The acute phase protein response is a well-described generalized early host response to tissue injury, inflammation and infection, observed as pronounced changes in the concentrations of a number of circulating serum proteins. The biological function of this response and its interplay with other parts of innate host defence reactions remain somewhat elusive. In order to gain new insight into this early host defence response in the context of bacterial infection we studied gene expression changes in peripheral lymphoid tissues as compared to hepatic expression changes, 14–18 h after lung infection in pigs. The lung infection was established with the pig specific respiratory pathogen Actinobacillus pleuropneumoniae. Quantitative real-time PCR based expression analysis were performed on samples from liver, tracheobronchial lymph node, tonsils, spleen and on blood leukocytes, supplemented with measurements of interleukin-6 and selected acute phase proteins in serum. C-reactive protein and serum amyloid A were clearly induced 14–18 h after infection. Extrahepatic expression of acute phase proteins was found to be dramatically altered as a result of the lung infection with an extrahepatic acute phase protein response occurring concomitantly with the hepatic response. This suggests that the acute phase protein response is a more disseminated systemic response than previously thought. The current study provides to our knowledge the first example of porcine extrahepatic expression and regulation of C-reactive protein, haptoglobin, fibrinogen, pig major acute phase protein, and transferrin in peripheral lymphoid tissues.

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Section: Discussionmentioning

confidence: 52%

Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs

et al. 2009

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“…There were no previous reports between above‐enumerated genes and traits of pigs except for ITIH2 , which is one of four members of inter‐α‐trypsin inhibitor heavy chains ( ITIH1 to 4 ). This gene family may play an important role in the maintenance of the uterine surface glycocalyx during placental attachment in pigs (Geisert et al . 2003).…”

Section: Resultsmentioning

confidence: 99%

Population structure of pigs determined by single nucleotide polymorphisms observed in assembled expressed sequence tags

Matsumoto¹,

Okumura²,

Uenishi

et al. 2011

Animal Science Journal

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We have collected more than 190000 porcine expressed sequence tags (ESTs) from full-length complementary DNA (cDNA) libraries and identified more than 2800 single nucleotide polymorphisms (SNPs). In this study, we tentatively chose 222 SNPs observed in assembled ESTs to study pigs of different breeds; 104 were selected by comparing the cDNA sequences of a Meishan pig and samples of three-way cross pigs (Landrace, Large White, and Duroc: LWD), and 118 were selected from LWD samples. To evaluate the genetic variation between the chosen SNPs from pig breeds, we determined the genotypes for 192 pig samples (11 pig groups) from our DNA reference panel with matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Of the 222 reference SNPs, 186 were successfully genotyped. A neighbor-joining tree showed that the pig groups were classified into two large clusters, namely, Euro-American and East Asian pig populations. F-statistics and the analysis of molecular variance of Euro-American pig groups revealed that approximately 25% of the genetic variations occurred because of intergroup differences. As the F(IS) values were less than the F(ST) values(,) the clustering, based on the Bayesian inference, implied that there was strong genetic differentiation among pig groups and less divergence within the groups in our samples.

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“…2a-h and Additional file 3, respectively). Literature search showed that most of these genes have known functions: tk2 [36] is a deoxyribonucleoside kinase that is required for mitochondrial DNA synthesis; drd3 [37] is dopamine receptor which is associated with cognitive, emotional, and endocrine functions; cldn23 [38] is an integral membrane protein that maintains cell polarity and signal transductions; nudt13 [39] is hydrolase and its function is also largely unknown; itih2 [40] is a serine protease inhibitor that carries hyaluronan in plasma; flvcr1 [41] is a heme transporter that has a critical role in erythropoiesis by protecting developing erythroid cells from heme toxicity; pomt1 [42] is an O-mannosyltransferase that is a component of many polysaccharides and glycoproteins; prkcq [43] is a serine- and threonine-specific protein kinase that is important for T-cell activation; and otulina [44] is a predicted deubiquitinase also called fam105ba . qPCR confirmed the differential expression of these genes in bad quality eggs relative to good quality eggs (Fig.…”

Section: Discussionmentioning

confidence: 99%

Lost in translation: egg transcriptome reveals molecular signature to predict developmental success and novel maternal-effect genes

Cheung

Tri

Cam

et al. 2018

Preprint

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BackgroundGood quality or developmentally competent eggs result in high survival of progeny. Previous research has shed light on factors that determine egg quality, however, large gaps remain. Initial development of the embryo relies on maternally-inherited molecules, such as transcripts, deposited in the egg, thus, they would likely reflect egg quality. We performed transcriptome analysis on zebrafish fertilized eggs of different quality from unrelated, wildtype couples to obtain a global portrait of the egg transcriptome to determine its association with developmental competence and to identify new candidate maternal-effect genes.ResultsFifteen of the most differentially expressed genes (DEGs) were validated by quantitative real-time PCR. Gene ontology analysis showed that enriched terms included ribosomes and translation. In addition, statistical modeling using partial least squares regression and genetics algorithm also demonstrated that gene signatures from the transcriptomic data can be used to predict reproductive success. Among the validated DEGs, otulina and slc29a1a were found to be increased in good quality eggs and to be predominantly localized in the ovaries. CRISPR/Cas9 knockout mutants of each gene revealed remarkable subfertility whereby the majority of their embryos were unfertilizable. The Wnt pathway appeared to be dysregulated in the otulina knockout-derived eggs.ConclusionsOur novel findings suggested that even in varying quality of eggs due to heterogeneous causes from unrelated wildtype couples, gene signatures exist in the egg transcriptome, which can be used to predict developmental competence. Further, transcriptomic profiling revealed two new potential maternal-effect genes that have essential roles in vertebrate reproduction.

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Expression of inter-alpha-trypsin inhibitor heavy chains in endometrium of cyclic and pregnant gilts

Cited by 23 publications

References 22 publications

Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs

Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs

Population structure of pigs determined by single nucleotide polymorphisms observed in assembled expressed sequence tags

Lost in translation: egg transcriptome reveals molecular signature to predict developmental success and novel maternal-effect genes

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