Expression of insulin-like growth factor I gene is involved in enhanced growth of juvenile four-spine sculpin Cottus kazika in seawater

Iwatani, Hozi; Inoue, Koji; Takei, Yoshiyuki

doi:10.1111/j.1444-2906.2005.01007.x

Cited by 4 publications

(2 citation statements)

References 20 publications

(41 reference statements)

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“…In the present study, the food supply was highest in the streams with elevated Se levels, so the slimy sculpin had an opportunity to increase their energy intake to meet any potential increase in energy demands of Se exposure. At the molecular level, increases in insulin‐like growth factor ( igf ) mRNA levels are known to be associated with growth in fish, including other sculpin species . However, the expression of igf could not be measured in the present study because the sequence was unavailable, and attempts to design primers failed.…”

Section: Discussionmentioning

confidence: 91%

Using molecular biomarkers and traditional morphometric measurements to assess the health of slimy sculpin (Cottus cognatus) from streams with elevated selenium in North‐Eastern British Columbia

Miller

Isaacs

Martyniuk

et al. 2015

Enviro Toxic and Chemistry

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Canadian fish-based environmental effects monitoring programs use individual and population-level endpoints to assess aquatic health. Impacts of coal mining and selenium (Se) exposure were assessed in slimy sculpin (Cottus cognatus) from reference streams located both inside and outside of a coal zone, and from 1 stream with a history of coal mining, using traditional environmental effects monitoring endpoints. In addition, physical characteristics of the streams and benthic macro-invertebrate communities were assessed. To determine whether the assessment of effects could be improved by including molecular markers, real-time polymerase chain reaction assays were optimized for genes associated with reproduction (vtg, esr1, star, cyp19a1, and gys2), and oxidative and cellular stress (sod1, gpx, gsr, cat, and hsp 90). Water Se levels exceeded guidelines in the stream with historical mining (4 μg/L), but benthic macroinvertebrates did not exceed dietary thresholds (2-3 μg/g dry wt). Whole-body Se levels were above British Columbia's tissue guideline in fish from all streams, but only above the draft US Environmental Protection Agency (USEPA) criterion (7.91 μg/g dry wt) at the reference stream inside the coal zone. Some markers of cellular and oxidative stress were elevated in fish liver at the exposed site (sod1, gpx), but some were lower (cat, sod1, gpx, gsr, hsp90) in the gonads of fish inside the coal zone. Some of the differences in gene expression levels between the reference and impacted sites were sex dependent. Based on benthic macroinvertebrate assessments, the authors hypothesize that traditional and molecular differences in slimy sculpin at impacted sites may be driven by food availability rather than Se exposure. The present study is the first to adapt molecular endpoints in the slimy sculpin for aquatic health assessments.

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Section: Discussionmentioning

confidence: 91%

Using molecular biomarkers and traditional morphometric measurements to assess the health of slimy sculpin (Cottus cognatus) from streams with elevated selenium in North‐Eastern British Columbia

Miller

Isaacs

Martyniuk

et al. 2015

Enviro Toxic and Chemistry

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“…2), reflecting reduced feed intake and growth for this treatment (Table 3). This enzyme exhibits pleiotropic effects: in addition to its major role in the mediation of growth and development, IGF1 is also involved in the response to salinity stress (Iwatani et al 2005;Meier et al 2009), the regulation of apoptosis and immune system activity (P erez-S anchez 2000). Hence, due its involvement in the response to various insults, IGF1 can be considered a mediator of allostasis (McEwen & Wingfield 2003.…”

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confidence: 99%

Effects of dietary purified rapeseed protein concentrate on hepatic gene expression in juvenile turbot (Psetta maxima)

Hermann

Reusch

Hanel³

2015

Aquacult Nutr

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Despite considerable progress in the production of alternative diets, small concentrations of antinutrients remain common in aquaculture nutrition, resulting in a perpetual limitation with regard to the inclusion of plant ingredients in aquafeeds. These compounds are known to impair the general performance of fish when fed for a prolonged period of time, potentially affecting the animal's susceptibility to stress, too. Therefore, a 12-week feeding trial was conducted to examine the chronic effects of purified rapeseed protein concentrate (RPC), containing low concentrations of glucosinolates and phytic acid, on the relative expression of multiple target genes in the liver of juvenile turbot (Psetta maxima, L.). Our results revealed divergent patterns of gene expression, suggesting different coping strategies dependent on the grade of RPC substitution. Data implies increased metabolic rate of turbot fed a 33% RPC-substituted diet due to an upregulation of cytochrome c oxidase mRNA, accompanied by minor adjustments in metabolic pathways. While no signs of reduced welfare were found, data adumbrate a beneficial hormetic reaction. In the highest treatment level (66% RPC), diminished fish condition and reduced growth performance coincided with a downregulation of insulin-like growth factor I, further indicating a potential impaired resistance to stress. An additional downregulation of transferrin hints towards an increased liability to bacterial infections.

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Increased growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor I (IGF-I) gene transcription after hyperosmotic stress in the Brazilian flounder Paralichthys orbignyanus

Meier

Figueiredo

Kamimura

et al. 2008

Fish Physiol Biochem

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Growth hormone (GH) action is the result of an intracellular cascade initiated just after its interaction with the growth hormone receptor (GHR) located on the surface of target cells. This cascade culminates with the transcription of target genes, such as the insulin-like growth factors (IGFs), which are responsible for most GH biological effects. In addition to its central role in growth, fish GH is also involved with osmoregulatory control. Within this context, the objective of the present work was to isolate GH, GHR, and IGF-I cDNAs from the Brazilian flounder Paralichthys orbignyanus and evaluate whether these genes are induced by hyperosmotic stress. The obtained results indicated that GH mRNA had a significant peak only 24 h after hyperosmotic stress. In gills, GHR mRNA was significantly increased after 7 days. In liver, GHR and IGF-I mRNAs were significantly increased in 72 h and both reached even higher levels after 7 days. These results indicate that hyperosmotic stress can increase GH sensitivity in the gills and liver of P. orbignyanus and, consequently, improve IGF-I production. The management of this parameter could be useful in achieving better growth performance for this and other commercially important species in which GH has a direct correlation with osmoregulatory mechanisms.

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Expression of insulin-like growth factor I gene is involved in enhanced growth of juvenile four-spine sculpin Cottus kazika in seawater

Cited by 4 publications

References 20 publications

Using molecular biomarkers and traditional morphometric measurements to assess the health of slimy sculpin (Cottus cognatus) from streams with elevated selenium in North‐Eastern British Columbia

Using molecular biomarkers and traditional morphometric measurements to assess the health of slimy sculpin (Cottus cognatus) from streams with elevated selenium in North‐Eastern British Columbia

Effects of dietary purified rapeseed protein concentrate on hepatic gene expression in juvenile turbot (Psetta maxima)

Increased growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor I (IGF-I) gene transcription after hyperosmotic stress in the Brazilian flounder Paralichthys orbignyanus

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