Expression of inhibin beta A and beta B, follistatin and activin-A receptor messenger ribonucleic acids in the rat seminiferous epithelium.

Kaipia, Antti; Penttilä, Tarja-Leena; Shimasaki, Shunichi; Ling, Nicholas; Parvinen, Martti; Toppari, Jorma

doi:10.1210/endo.131.6.1332846

Cited by 90 publications

(52 citation statements)

References 25 publications

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“…This proposal is supported by the results of a previous study demonstrating that GATA-1 transactivates the inhibin/ activin b-B-subunit gene (11). FOG-1 might well act as a GATA-1 repressor in its action on the inhibin/ activin b-B-subunit, since the expression of this gene is lowest in Sertoli cells at stages VII -XII of the seminiferous epithelial cycle (38). It is of interest to note that not only GATA-1 but also GATA-4 have been shown to transactivate the inhibin/activin b-B-subunit gene in Sertoli and Leydig cell lines (11).…”

Section: Discussionsupporting

confidence: 62%

Developmental expression and spermatogenic stage specificity of transcription factors GATA-1 and GATA-4 and their cofactors FOG-1 and FOG-2 in the mouse testis

Ketola

Anttonen

Vaskivuo

et al. 2002

European Journal of Endocrinology

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Objective: The transcription factors GATA-1 and GATA-4 have been implicated in the regulation of testicular development and function. Their cofactors FOG-1 and FOG-2 are expressed in the gonads, but their cell-specific and developmental expression in the testis remains unresolved. Therefore, we analyzed GATA-1, GATA-4, FOG-1 and FOG-2 expression in detail, from undifferentiated male urogenital ridge to adult testis. Methods: Immunohistochemistry and in situ hybridization were applied on mouse testicular samples. Results: GATA-4 and FOG-2, but not GATA-1 or FOG-1, were expressed as early as in the male urogenital ridge. FOG-2 expression was localized in the Sertoli cells at embryonal day 12.5 (E12.5), but it diminished with advancing fetal testicular development. In E17.5 testis, FOG-2 was present only in the testicular capsule and a subset of fetal Leydig cells. FOG-1 was expressed from E15.5 Sertoli cells onwards, whereas GATA-1 was not detected during the fetal period at all. In the postnatal testis, FOG-2 was abundantly expressed immediately after birth, but in adult testis its expression was predominantly restricted to stage VII -XII seminiferous tubules. Stage specificity was also found for FOG-1, which, similarly to GATA-1, was abundantly expressed in stage VII -XII tubules during adulthood. Conclusions: Our results indicate that FOG-2, in addition to GATA-4, has a role in early gonadal development and sexual differentiation, and FOG-1 at later fetal stages, while GATA-1 executes its action postnatally. The findings suggest that, in contrast to the hematopoietic system and the heart, GATA-1 and GATA-4 do not use FOG-1 and FOG-2 respectively as their only cofactors during the early stages of testicular development.

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Section: Discussionsupporting

confidence: 62%

Developmental expression and spermatogenic stage specificity of transcription factors GATA-1 and GATA-4 and their cofactors FOG-1 and FOG-2 in the mouse testis

Ketola

Anttonen

Vaskivuo

et al. 2002

European Journal of Endocrinology

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“…The anti-ActRI antibody clearly recognized type I activin receptor after incubation with peptide Nglycosidase F (PNGase F) (10). Furthermore, these antibodies can be used for immunohistochemistry: gonads, brain and pancreatic islets, the tissues known to express activin and activin receptors, were stained positively by this method (6,10,12,13), and the immunodistributions of activin b A molecules in testis and ActRII molecules in brain were consistent with the expression of mRNA evaluated by in situ hybridization (10,12,14,15).…”

Section: Methodsmentioning

confidence: 64%

Expression and localization of activin receptors during endochondral bone development

Funaba

Ogawa

Abe

2001

European Journal of Endocrinology

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The expression and localization of activins (dimeric protein of inhibin b subunit) and activin receptors in skeletal tissue were examined. RT-PCR revealed that cultured chondrocytes expressed mRNAs of inhibin/activin b A and four activin receptors (two type I (ActRI and ActRIB) and two type II (ActRII and ActRIIB)). Immunohistochemical analyses showed that activin b A , ActRI and ActRII were localized in proliferating chondrocytes and osteoblasts in tibiae of neonatal rats, and in implants of demineralized bone matrix, a well-established model of ectopic bone formation. The immunoreactivities of osteoblasts were decreased with aging in the tibiae and with progressing endochondral bone development in the implants. The strong expression of ActRI was also detected in hypertrophic chondrocytes both in the tibial growth plate and in the implants, whereas immunoreactive ActRII was lower in hypertrophic chondrocytes. Western blot analysis also showed that immunoreactive ActRI, migrating at 52 kDa, was detected only in the implants on days 9 and 11, the period of conversion from cartilage to bone. In view of the sharing of type II receptors between activins and bone morphogenetic proteins (BMPs), our findings suggest that activin/BMP activity involves in bone modeling, especially during active chondro-and osteogenesis and during the conversion from cartilage to bone.

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“…In the rat, FSH regulates mRNA of the a-but not [3-B subunit of inhibin (Krummen et al, 1989). Activin controls the proliferation of rat spermatogonia (Mather and Krummen, 1992;Kaipia et al, 1992Kaipia et al, , 1994. Furthermore, an activin (3-neutralizing antiserum blocks activin [3-stimulated 3 H-thymidine incorporation in spermatogonia (Mather etal, 1994).…”

Section: Histologymentioning

confidence: 99%

“…Furthermore, an activin (3-neutralizing antiserum blocks activin [3-stimulated 3 H-thymidine incorporation in spermatogonia (Mather etal, 1994). Activin receptors (Act RII) were detected in type A spermatogonia in rodents (Kaipia et al, 1992(Kaipia et al, , 1994.…”

Section: Histologymentioning

confidence: 99%

Effect of short photoperiodic cycles on male genital tract and testicular parameters in male goats (Capra hircus)

Delgadillo¹,

Reviers²,

Daveau³

et al. 1995

Reprod. Nutr. Dev.

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Summary ― This study was performed in adult male goats in which seasonal variations were abolished by rapid alternations of long days and short days. These treatments have been shown previously to prevent seasonal changes in the hypothalamo-pituitary axis and to maintain testis weight and sperm production at a high level. The experimental groups were exposed for 3 years to an alternation of either a 1 month short (16 h dark; 8 h light) and 1 month long (16 L; 8 D) photoperiod (2 month cycle; n = 5) or of a 2 month short and 2 month long photoperiod (4 month cycle; n = 4). The control groups were maintained in natural photoperiodic conditions (45°N) and goats were slaughtered in the non-breeding season (end of April RS; n = 5) at the same period as light-treated bucks, or in the breeding season (end of September BS; n = 6). The total weight of the testes, the length and mean diameter of the seminiferous tubules of light-treated goats were similar to those in the breeding season, and higher than those in the non-breeding season. The total number of A o spermatogonia was increased by light treatments as compared to control goats in the breeding and non-breeding season. The daily production of A, spermatogonia, leptonene primary spermatocytes and round spermatids in light-treated goats was maintained at the peak breeding season level. The intra-testicular concentration of testosterone, total volumes of intertubular tissue and of Leydig cells, and the number of Leydig cells per testis did not differ between groups. Although the mean cross-sectional area of Leydig cells in light-treated goats was similar to this area in non-breeding season goats, it was significantly lower than that of breeding season goats. In conclusion, the rapid alternation of short and long days allowed an increase in all the germ cells from the A o spermatogonia onwards, which was responsible for the maintenance of high spermatogenetic activity of light-treated goats.

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Expression of inhibin beta A and beta B, follistatin and activin-A receptor messenger ribonucleic acids in the rat seminiferous epithelium.

Cited by 90 publications

References 25 publications

Developmental expression and spermatogenic stage specificity of transcription factors GATA-1 and GATA-4 and their cofactors FOG-1 and FOG-2 in the mouse testis

Developmental expression and spermatogenic stage specificity of transcription factors GATA-1 and GATA-4 and their cofactors FOG-1 and FOG-2 in the mouse testis

Expression and localization of activin receptors during endochondral bone development

Effect of short photoperiodic cycles on male genital tract and testicular parameters in male goats (Capra hircus)

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