Expression of inflammation-related cytokines following intratracheal instillation of nickel oxide nanoparticles

Morimoto, Yasuo; Ogami, Akira; Todoroki, Motoi; Yamamoto, Makoto; Murakami, Masahirô; Hirohashi, Masami; Oyabu, Takako; Myojo, Toshihiko; Nishi, Kenichiro; Kadoya, Chikara; Yamasaki, Sayumi; Nagatomo, Hiroko; Fujita, Katsuhide; Endoh, Shigehisa; Uchida, Kunio; Yamamoto, Kazuhiro; Kobayashi, Norihiro; Nakanishi, Junko; Tanaka, Isamu

doi:10.3109/17435390903518479

Cited by 74 publications

(65 citation statements)

References 35 publications

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“…This later event is induced by secretion of proinflammatory cytokines and subsequent macrophage accumulation. Actually, at 3 days after intratracheal instillation of NiO nanoparticles, the secretion of inflammatory cytokines, and infiltration of alveolar macrophages and neutrophils were observed 44) . We consider that HO-1 expression was reduced with the weakening of the primary oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Acute Oxidative Stress Induced by NiO Nanoparticles In Vivo and In Vitro

Horie

Fukui

Nishio

et al. 2011

Journal of Occupational Health

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Evaluation of Acute Oxidative Stress Induced by NiO Nanoparticles In Vivo and In Vitro:Masanori Horie, et al. Health Research Institute -Objectives: Nickel oxide (NiO) is an important industrial material, and it is also a harmful agent. The toxicity of NiO is size-related: nanoparticles are more toxic than fine-particles. The toxic mechanism induced by NiO nanoparticles remains unexplained, and the relationship between in vitro and in vivo NiO toxicity results is unclear. In the present study, we focused on the oxidative stress caused by NiO nanoparticles by examining and comparing in vitro and in vivo acute responses induced by NiO nanoparticles. Methods: Cellular responses induced by black NiO nanoparticles with a primary particle size of 20 nm, were examined in human lung carcinoma A549 cells. In vivo responses were examined by instillation of NiO nanoparticles into rat trachea. Bronchoalveolar lavage fluid (BALF) was collected after intratracheal instillation at different time points, and concentrations of lipid peroxide heme oxygenase-1 (HO-1), surfactant protein-D (SP-D) and lactate dehydrogenase (LDH) in BALF were measured. Results: The levels of intracellular reactive oxygen species and lipid peroxidation in A549 cells increased with increasing exposure to NiO nanoparticles, and increases in gene expressions of HO-1 and SP-D were observed in A549 cells. The lipid peroxide level in BALF significantly increased after 24 h instillation but decreased three days later. LDH leakage was also observed three days later. Conclusions: NiO nanoparticles induce oxidative stress-related lung injury. In vivo and in vitro oxidative stress was induced resulting in activation of antioxidant systems. Based on these responses, we conclude that the results of the in vivo and in vitro studies tend to correspond. (J Occup Health 2011; 53: 64-74)

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Section: Discussionmentioning

confidence: 99%

“…It was reported that intratracheal instillation of NiO nanoparticles induced expression of inflammation-related cytokines 44) . In the present study, the relationship between the oxidative stress induced by NiO particles in vitro and in vivo was examined.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Acute Oxidative Stress Induced by NiO Nanoparticles In Vivo and In Vitro

Horie

Fukui

Nishio

et al. 2011

Journal of Occupational Health

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100

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“…With regard to the inflammatory effect of NiO, we carried out an intratracheal instillation study of nanoscale NiO nanoparticles and reported that they caused persistent pulmonary inflammation with a low dose 13,14) . Lu et al 15) also reported that NiO nanoparticles caused pulmonary inflammation in their intratracheal instillation study in mice.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Gene Expression of MMP-2 and TIMP-2 mRNA in Rat Lung in Inhaled Nickel Oxide and Titanium Dioxide Nanoparticles

et al. 2011

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In order to investigate whether or not dispersed nanoparticles have an effect of inflammation and fibrosis on animals, we developed a nanoparticle generation system and examined the gene expression of matrix metalloproteinase (MMP) and tissue inhibitor matrix proteinase (TIMP) in rat lung containing inhaled nickel oxide (NiO) or titanium dioxide (TiO 2 ) nanoparticles. In both experiments, Wistar male rats were exposed to NiO or TiO 2 nanoparticles for 4 wk (6 h/day). The geometric mean diameters of NiO and TiO 2 in the chamber were 139 ± 12 nm and 51 ± 9 nm, respectively. The average concentration of the particle number of NiO and TiO 2 was 1.0E+05 /cm 3 and 2.8E+05 /cm 3 , respectively. At 4 d, 1 and 3 months after the end of the inhalation, the rats exposed to these particles were sacrificed and the gene expressions of MMP-2, TIMP-2 and type I collagen were measured using RT-PCR. Pathological finding revealed that there was minimum inflammation with nickel oxide only at 4 d and no change with titanium oxide. However, there were no changes of the gene expression of MMP-2, TIMP-2, and type I collagen in either the NiO or TiO 2 exposure groups. In this study, inhalation of nickel oxide and titanium dioxide nanoparticles did not induce the gene expression of MMP-2 and TIMP-2 mRNA in rat lungs.

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“…4, Table 2). Several nanomaterials have been used to examine the relationship between the toxicity of nanomaterials and pulmonary inflammation [15,24]. NiO [25] and CeO 2 [9], nanoparticles with high toxicity considered in the data of inhalation tests, induced sustained changes in the above markers in the BALF, and TiO 2 , fullerene [7] and ZnO [8], nanoparticles with low toxicity considered in the data of inhalation tests, induced only transient changes.…”

Section: Endpointsmentioning

confidence: 99%

Significance of Intratracheal Instillation Tests for the Screening of Pulmonary Toxicity of Nanomaterials

Morimoto

Izumi

Yoshiura

et al. 2017

J UOEH

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: Inhalation tests are the gold standard test for the estimation of the pulmonary toxicity of respirable materials. Intratracheal instillation tests have been used widely, but they yield limited evidence of the harmful effects of respirable materials. We reviewed the effectiveness of intratracheal instillation tests for estimating the hazards of nanomaterials, mainly using research papers featuring intratracheal instillation and inhalation tests centered on a Japanese national project. Compared to inhalation tests, intratracheal instillation tests induced more acute inflammatory responses in the animal lung due to a bolus effect regardless of the toxicity of the nanomaterials. However, nanomaterials with high toxicity induced persistent inflammation in the chronic phase, and nanomaterials with low toxicity induced only transient inflammation. Therefore, in order to estimate the harmful effects of a nanomaterial, an observation period of 3 months or 6 months following intratracheal instillation is necessary. Among the endpoints of pulmonary toxicity, cell count and percentage of neutrophil, chemokines for neutrophils and macrophages, and oxidative stress markers are considered most important. These markers show persistent and transient responses in the lung from nanomaterials with high and low toxicity, respectively. If the evaluation of the pulmonary toxicity of nanomaterials is performed in not only the acute but also the chronic phase in order to avoid the bolus effect of intratracheal instillation and inflammatory-related factors that are used as endpoints of pulmonary toxicity, we speculate that intratracheal instillation tests can be useful for screening for the identification of the hazard of nanomaterials through pulmonary inflammation.

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Expression of inflammation-related cytokines following intratracheal instillation of nickel oxide nanoparticles

Cited by 74 publications

References 35 publications

Evaluation of Acute Oxidative Stress Induced by NiO Nanoparticles In Vivo and In Vitro

Evaluation of Acute Oxidative Stress Induced by NiO Nanoparticles In Vivo and In Vitro

Investigation of Gene Expression of MMP-2 and TIMP-2 mRNA in Rat Lung in Inhaled Nickel Oxide and Titanium Dioxide Nanoparticles

Significance of Intratracheal Instillation Tests for the Screening of Pulmonary Toxicity of Nanomaterials

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