Expression of inducible nitric oxide synthase by Corydalis turtschaninovii on interferon-γ stimulated macrophages

Influence of Vinegar and Wine Processing on the Alkaloid Content and Composition of the Traditional Chinese Medicine Corydalis Rhizoma (Yanhusuo)

“…The two most abundant alkaloids were tetrahydrocolumbamine (3) and dehydrocorydaline (7). Together, the two alkaloids accounted for 46%-53% of the total alkaloid content in the three analyzed extracts.…”

“…Alkaloids such as tetrahydropalmatine exert their pharmaceutical effect by interfering with neurotransmitters in the central nervous system [3][4][5][6]. Other biological activities of the alkaloids or crude extract from Corydalis Rhizoma were also found, such as anti-tumor activity [7][8][9], anti-inflammatory and analgesic activity [10][11][12], hepatoprotective effects [13], and antiplasmodial activity [14]. Several high performance liquid chromatography (HPLC) methods have been reported for the analysis of alkaloids in C. yanhusuo.…”

Drynariae Rhizoma Increases Immune Response in Mice

“…Peritoneal macrophages (3 × 10 5 cells/well) were cultured with rIFN-γ (10 U/mL) for 6 h. The cells were then stimulated with various concentrations of TO. NO synthesis in cell cultures was measured by a microplate assay method as previously described [20]. To measure nitrite, 100 μL aliquots were removed from conditioned medium and incubated with an equal volume of Griess reagent (1% sulphanilamide/0.1% N-(1-naphtyl)-ethylenediamine dihydrochloride/2.5% H 3 PO 4 ) at room temperature for 10 min.…”

Anti-inflammatory effects of Cheongilppong on DSS-induced ulcerative colitis in mice

“…Peritoneal macrophages (3×10 5 cells/well) were pretreated with CIPB (0.1-2 mg/ml) for 1 h and then cultured with rIFN-γ (10 U/ml) for 6 h, after which they were stimulated with LPS (1 μg/ml) for 48 h. The cells were then stimulated with various concentrations of ST. NO synthesis in cell cultures was measured by a microplate assay method as previously described (An et al 2009). To measure the nitrite concentration, 100 μl aliquots were removed from conditioned medium and incubated with an equal volume of Griess reagent (1% sulfanilamide/0.1% N-(1-naphtyl)-ethylenediamine dihydrochloride/2.5% H 3 PO 4 ) at room temperature for 10 min.…”