Expression of IL-2α and IL-2β Receptors on the Membrane Surface of Human Sperm

Fierro, Reyna; Schwed, P; Foliguet, B; Grignon, G; Béné, Marie C.; Fauré, G

doi:10.1080/01485010290099264

Cited by 4 publications

(4 citation statements)

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“…Interleukin 2 α and β Receptors . Flow cytometry and transmission electron microscopy detected interleukin 2 (IL2) α and β receptors in head and midpiece regions of human spermatozoa; these receptors have shown some correlation with sperm concentration in semen (Fierro et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Receptors in Spermatozoa: Are They Real?

Naz

Sellamuthu

2006

Journal of Andrology

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Section: Resultsmentioning

confidence: 99%

Receptors in Spermatozoa: Are They Real?

Naz

Sellamuthu

2006

Journal of Andrology

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“…In the same experiment, adding anti IL‐6 R antibody abolished these adverse effects (Yoshida et al, 2004). Cytokine receptors were identified and localized on human sperm and correlated with poor sperm concentration in 20 oligospermic infertile men attending an in vitro fertilization program (Fierro et al, 2002). During genital infection, cytokines and various soluble receptors of immunoregulatory cytokines were expressed distinctly in seminal plasma (Huleihel et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Neutralization of Cytokine Activity at the Receptor Level Improves Sperm Motility in Men With Spinal Cord Injuries

Brackett

Cohen

Ibrahim

et al. 2007

Journal of Andrology

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Men with spinal cord injury (SCI) have a unique semen profile characterized by normal sperm concentrations but abnormally low sperm motility. Previous studies showed that elevated concentrations of cytokines in the seminal plasma of these men contribute to this condition. For example, when elevated concentrations of interlekin-1b (IL-1b), IL-6, and tumor necrosis factor-a (TNF-a) were immunoneutralized in the semen of men with SCI, sperm motility improved. The present study investigated if these cytokines act on sperm cell receptors to inhibit sperm motility. Semen was collected from men with SCI and from healthy non-SCI men. Sperm were separated from the seminal plasma by centrifugation. Eight identical aliquots of 5000 sperm suspended in 50 mL of seminal plasma were prepared for each subject. Agents were added to the aliquots in order to neutralize IL-1b, IL-6, and TNF-a at the receptor level. In SCI subjects, sperm motility improved in each treatment group compared with the untreated group, but statistical significance was reached only when neutralizing agents to all 3 cytokines were added. Improvement was less pronounced in subjects with close to normal semen cytokine concentrations or close to normal pretreatment sperm motility. In control subjects, IL1b, IL-6, and TNF-a were within normal values, and addition of receptor blockers to semen had no effect on sperm motility. These data support the hypothesis that cytokines act at the level of the sperm receptor to inhibit sperm motility. These data further support the notion that inactivating semen cytokines leads to improved sperm motility in SCI men. Our goal is to develop this finding into a treatment for low sperm motility in men with SCI.Key words: Infertility, ejaculation, semen, TNF-a, IL-1b, and IL-6. J Androl 2007;28:717-721 M en with spinal cord injury (SCI) have a unique semen profile characterized by normal sperm concentration but abnormally low sperm motility (Brackett et al, 1996(Brackett et al, , 1997DeForge et al, 2005;Brown et al, 2006). Factors in the seminal plasma contribute to this condition. For example, previous studies showed elevated concentrations of white blood cells and activated T-cell subpopulations in the semen of men with SCI (Basu et al, 2002). Additionally, elevated concentrations of cytotoxic cytokines were found in the seminal plasma of these men . When the elevated cytokine concentrations were immunoneutralized with monoclonal antibodies added to the semen, sperm motility improved (Cohen et al, 2004).The present study was undertaken to determine if cytokine receptors are involved in mediating the effects of cytokines on sperm motility in men with SCI. Materials and Methods SubjectsSubjects were men with traumatic SCI and healthy non-SCI subjects. All subjects were participants in the Male Fertility Research Program of the Miami Project to Cure Paralysis at the University of Miami Miller School of Medicine, Miami, Fla. The mean (6 SEM) age of SCI subjects was 31.9 6 2.3 years (range, 20-42). All SCI subjects were ...

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“…Possible causes for these observations are topics for further investigation. For example, in the group with the highest mean PSM (41.6% ± 2.9%), with motility close to normal, other mechanisms at play might be higher concentrations of stimulatory cytokines, lower concentrations of reactive oxygen species, or different cytokine receptor expression (Fierro et al, 2002). For the group with the lowest mean PSM (1.6% ± 1.6%), these low sperm motilities might represent overwhelming irreparable damage related to any of the above‐mentioned factors or simply to higher concentrations of toxic cytokines in the seminal plasma.…”

Section: Discussionmentioning

confidence: 99%

Sperm Motility in Men With Spinal Cord Injuries Is Enhanced by Inactivating Cytokines in the Seminal Plasma

Cohen

Basu

Randall

et al. 2004

Journal of Andrology

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ABSTRACT:The goal of this study was to determine whether inactivating specific cytokines in seminal plasma improves sperm motility in men affected by spinal cord injury (SCI). For this purpose, we used monoclonal antibodies to interleukin 6 (IL6), interleukin 1 beta (IL1-␤), and tumor necrosis factor alpha (TNF-␣), all 3 cytokines having been previously detected at high concentrations in the seminal plasma of patients with SCI. In a group of 17 SCI men with low sperm motility (mean Ϯ SE, 20.1% Ϯ 3.1%), treatment with the 3 monoclonal antibodies at the median neutralization dose concentrations for 1.0 to 1.5 hours improved sperm motility in all cases. Effectiveness was higher in those specimens with a pretreatment sperm motility between 11% and 30% (from 19.3% Ϯ 1.4% to 41.9% Ϯ 4.9%, P Ͻ .0002), suggesting that pretreatment sperm motility might represent an indicator of cell damage and, therefore, a factor that influences monoclonal antibody effectiveness. To the best of our knowledge, these results represent the first rational treatment for improving low sperm motility in these severely affected patients.Key words: Infertility, ejaculation, semen, IL1-␤, IL6, TNF-␣. J Androl 2004;25:922-925P atients with spinal cord injury (SCI) have impaired sexual function and a unique sperm profile characterized by normal to high sperm concentrations and abnormally low sperm motility (Brackett et al, 1996b(Brackett et al, , 1997. The cause of this condition is unknown but might be related to abnormalities in the seminal plasma. Activated Tcell subpopulations were identified in the semen of these patients (Basu et al, 2002), and abnormal concentrations of cytotoxic cytokines were found in seminal plasma of SCI men with low sperm motility (Basu et al, 2004). To determine whether elevated cytokines contribute to low sperm motility in men with SCI, this study evaluated the usefulness of specific monoclonal antibodies to improve sperm motility by blocking cytokine activity in fresh semen samples. Miami, Fla). The mean (ϮSEM) age of subjects was 35.2 Ϯ 2.2 years (range 21 to 43 years). All subjects were past the acute phase of injury, and their mean time postinjury was 13.3 Ϯ 3.8 years (range 3 to 32 years). Levels of injury were C5 to C6 in 5 subjects, T1 to T7 in 6 subjects, and T8 to T12 in 6 subjects. Each subject had undergone at least 4 ejaculations spaced 4 to 8 weeks apart prior to semen collection for this study. All subjects were in good health and did not have any condition, other than SCI, known to interfere with fertility. Materials and Methods Subjects Semen Collection and AnalysisOnly antegrade semen (ie, no retrograde semen) was collected from subjects by the standard method of penile vibratory stimulation (Brackett, 1999) because semen quality can be altered in retrograde ejaculates or if semen is collected by electroejaculation . Semen analysis was performed according to World Health Organization criteria (1999). Each semen specimen was first allowed to liquefy at room temperature. Sperm parameters were assessed b...

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Expression of IL-2α and IL-2β Receptors on the Membrane Surface of Human Sperm

Cited by 4 publications

References 20 publications

Receptors in Spermatozoa: Are They Real?

Receptors in Spermatozoa: Are They Real?

Neutralization of Cytokine Activity at the Receptor Level Improves Sperm Motility in Men With Spinal Cord Injuries

Sperm Motility in Men With Spinal Cord Injuries Is Enhanced by Inactivating Cytokines in the Seminal Plasma

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