Expression of IL-23 and IL-17 and effect of IL-23 on IL-17 production in ankylosing spondylitis

Abstract

The objective of this study was to investigate the expression of IL-23 and IL-17 and the influence of IL-23 on IL-17 production in ankylosing spondylitis (AS) patients. IL-23 and IL-17 levels in the serum and supernatants of cultured peripheral blood mononuclear cells (PBMCs) were determined by ELISA. IL-23p19 mRNA expression in PBMCs were analyzed using RT-PCR. The patients with AS at active stage showed elevated levels of IL-23 and IL-17 in the serum and supernatants of cultured PBMCs. A higher expression of IL-23p19 mRNA in PBMCs of AS patients was also observed. A significantly enhanced production of IL-17 in the supernatants of cultured PBMCs was found in the presence of recombinant IL-23 and this effect was more significant in patients with AS. The results suggest that IL-23 and IL-17 may play critical roles in the pathogenesis of AS and IL-23-stimulated production of IL-17 by PBMCs may be responsible for the development of AS.

Protective role of R381Q (rs11209026) polymorphism in IL-23R gene in immune-mediated diseases: A comprehensive review

“…In humans, IL-23 is over-expressed in clinical samples of psoriasis (Lee et al 2004), CD (Schmidt et al 2005) and ankylosing spondylitis (Wang et al 2009). Becker et al (2006) showed that blocking the p40 subunit of either IL-12 or IL-23, but not blocking the p19 unit (Ferguson et al 2010), was effective in reducing disease symptoms in chemically-induced colitis models.…”

Interleukin‐23 Mediates the Intestinal Response to Microbial β‐1,3‐Glucan and the Development of Spondyloarthritis Pathology in SKG Mice

“…Moreover, studies in humans show that production of and sensitivity to IL-23 are increased and IL-23-responsive cells are expanded in many forms of SpA (9,(12)(13)(14), together strongly implicating IL-23 and the cells upon which it acts in the pathogenesis of SpA and IBD. In support of these findings, overexpression of IL-23 by hepatic injection of Il23 minicircles was recently shown to be sufficient to drive enthesitis and aortitis in mice through IL-23Rϩ innate lymphoid enthesealresident cells in a process involving IL-22 and IL-17 (15).…”

IL-23 and IL-17 in ankylosing spondylitis

“…Moreover, for the first time, a higher expression of IL-23 p19 mRNA in PBMC of AS patients as well as enhanced production of IL-17 in the supernatants of PBMC cultured in the presence of recombinant IL-23 was found by Wang et al [1]. Previous studies did not demonstrate elevated levels of p40 IL-23 in the serum of AS patients, compared with controls, whereas, in some of them, synovial fluid levels were higher than serum levels [7].…”

“…According to the results of Wang et al [1], one may speculate that p19 IL-23 blockade may be even more efficacious, and may represent a new therapeutic pathway in AS.…”

“…We read with interest the study published by Wang et al demonstrating the elevated levels of IL-23 and IL-17 in the serum of patients with active Ankylosing Spondylitis (AS) [1].…”