Expression of icaA and icaD genes in biofilm formation in Staphylococcus aureus isolates from bovine subclinical mastitis

Marques, Viviane Figueira; Santos, Huarrisson Azevedo; Santos, Thomas H; Melo, Dayanne Araújo de; Coelho, S.M.O.; Coelho, Irene da Silva; Souza, Miliane Moreira Soares de

doi:10.1590/1678-5150-pvb-6645

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Additionally, regarding the ways via which binding has expressed the role of proteins in staphylococci biofilms, a clarification is required [21]. According to Marques et al [22], the relative mRNA expression of the ica A and D genes in the strong biofilm-producer was more elevated in 24 hours and less expressed in moderate and weak isolates after 24 hours. Whereas icaD expression in moderate isolate recorded the lowest expression [22].…”

Section: Tablementioning

confidence: 99%

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Gene Expression Evaluation of Intracellular Adhesins and Regulatory Genes among Biofilm Producing MRSA Isolates

Abdrabaa

Aburesha

2023

Iraqi Journal of Science

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The gene expression of the most important structural genes ica A and D of biofilm, sarA, and sigB regulatory genes of some methicillin-resistant Staphylococcus aureus (MRSA) isolates were examined using the real-time polymerase chain reaction after 24 hours of growth. The results revealed that the isolates with strong biofilm production had the highest gene expression of the structural icaA and D genes. Whereas the isolates that showed moderate and weak biofilm production, recorded the lowest gene expression. The results of the regulatory genes sarA, and sigB fluctuated among all MRSA isolates. Isolate No. 64 recorded the highest gene expression, while isolate 50 recorded the lowest gene expression. The purpose of this study was to determine the gene expression of structural genes ica A and D, and regulator genes sigB and sarA in some MRSA isolates with different abilities of biofilm formation where the sarA and sigB play important role in positive regulation of biofilm formation.

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Section: Tablementioning

confidence: 99%

“…According to Marques et al [22], the relative mRNA expression of the ica A and D genes in the strong biofilm-producer was more elevated in 24 hours and less expressed in moderate and weak isolates after 24 hours. Whereas icaD expression in moderate isolate recorded the lowest expression [22].…”

Section: Tablementioning

confidence: 99%

Gene Expression Evaluation of Intracellular Adhesins and Regulatory Genes among Biofilm Producing MRSA Isolates

Abdrabaa

Aburesha

2023

Iraqi Journal of Science

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Biofilm Associated Uropathogenic Escherichia coli from Catheterised Patients at a Nigerian Hospital

Yaki,

Nwabuisi,

Dagang

et al. 2024

Preprint

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Catheter-associated urinary tract infections (CAUTIs) pose a significant health risk in healthcare facilities, accounting for 40% of all hospital-acquired infections and 80% of healthcare-acquired urinary tract infections. These infections are often associated with biofilm formation, which can lead to recurrent or persistent infections due to antimicrobial resistance. In this study, urine samples from 230 catheterised patients, mostly males (91.3%), were analysed to investigate the prevalence of biofilm-forming uropathogenic E. coli (UPEC) and their correlation with antibiotic resistance. Standard microbiological methods and the Analytical Profile Index (API) 20E were used to isolate and identify UPEC, while the microtiter plate method was used to screen UPEC for their ability to form biofilms under static and dynamic conditions. An antimicrobial susceptibility test was also performed using a modified Kirby-Bauer disc diffusion method. The results of the study showed that UPEC was present in 21.7% of catheterized urine samples, with patients with long-term catheterization accounting for 96% of these isolates. Among these isolates, 84% and 86% were found to form biofilms under static and dynamic conditions, respectively, with all strong biofilm-forming isolates being extensively drug-resistant. The study also found a significant correlation between biofilm formation and resistance to most antibiotics tested, including Augmentin, Ceftazidime, Ceftriaxone, Gentamicin, and Ciprofloxacin (p-value < 0.05). However, Imipenem and nitrofurantoin showed no significant correlation with biofilm formation (p-value > 0.05). Given these findings, it is recommended that routine tests include biofilm detection and antimicrobial susceptibility assessments to guide the choice of a suitable antibiotic treatment, which is essential for effective management.

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A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism

et al. 2022

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Bacteria can form biofilms in natural and clinical environments on both biotic and abiotic surfaces. The bacterial aggregates embedded in biofilms are formed by their own produced extracellular matrix. Staphylococcus aureus (S. aureus) is one of the most common pathogens of biofilm infections. The formation of biofilm can protect bacteria from being attacked by the host immune system and antibiotics and thus bacteria can be persistent against external challenges. Therefore, clinical treatments for biofilm infections are currently encountering difficulty. To address this critical challenge, a new and effective treatment method needs to be developed. A comprehensive understanding of bacterial biofilm formation and regulation mechanisms may provide meaningful insights against antibiotic resistance due to bacterial biofilms. In this review, we discuss an overview of S. aureus biofilms including the formation process, structural and functional properties of biofilm matrix, and the mechanism regulating biofilm formation.

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Expression of icaA and icaD genes in biofilm formation in Staphylococcus aureus isolates from bovine subclinical mastitis

Cited by 3 publications

References 29 publications

Gene Expression Evaluation of Intracellular Adhesins and Regulatory Genes among Biofilm Producing MRSA Isolates

Gene Expression Evaluation of Intracellular Adhesins and Regulatory Genes among Biofilm Producing MRSA Isolates

Biofilm Associated Uropathogenic Escherichia coli from Catheterised Patients at a Nigerian Hospital

A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism

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