EXPRESSION OF Ia ANTIGENS ON MURINE MACROPHAGES

Cowing, Carol; Dickler, Howard B.

doi:10.1016/b978-0-12-483260-2.50022-7

Cited by 12 publications

(13 citation statements)

References 3 publications

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“…Conversely, the Ia-antigen expression by unstimulated peritoneal macrophages declined in culture. This observation supports the findings of Cowing and Dickler (2) and suggests that Ia ÷ macrophages convert to Ia-cells in the absence of regulatory factors.…”

Section: Discussionsupporting

confidence: 81%

Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells.

Steeg¹,

Moore²,

Oppenheim³

1980

The Journal of Experimental Medicine

151

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This investigation examined the effects of mediators derived form activated spleen cells on macrophage Ia-antigen expression and function. Incubation of adherent thioglycollate-induced murine peritoneal macrophages(> 90% Ia-) with concanavalin A (Con A)-stimulated spleen cell supernate (Con A sup) resulted in a dose-dependent increase in the percentage of Ia-containing (Ia+) phagocytic cells, as detected by antiserum-and-complement-mediated cytotoxicity. The Ia-antigen expression of macrophages incubated with unstimulated spleen cell supernate supplemented with Con A (Control sup) declined. Pretreatment of the macrophages with anti-Ia and complement before addition of the Con A sup did not inhibit subsequent Ia-antigen expression, suggesting that Ia- macropohages were converted to Ia+ cells. These findings were not a result of adsorption of soluble Ia-antigen from the Con A sup, because Ia-antigen expression was detected by an antiserum specific for the haplotype of the macrophages but not that of the allogeneic spleen cells from which the supernate was prepared. Con A sup-cultured macrophages also stimulated the proliferation of allogeneic spleen cells significantly better than Control sup-cultured macrophages in the mixed leukocyte reaction (MLR). Pretreatment of Con A sup-cultured macrophages with anti-Ia and complement before addition of splenic responder cells abrogated their stimulatory capacity, indicating the Ia dependence of the MLR. We hypothesize that regulatory lymphokine(s) can induce both the expression of the Ia+ phenotype by macrophages and the functional capability to stimulate the MLR, and that macrophages lose these capabilities in the absence of such mediator(s).

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Section: Discussionsupporting

confidence: 81%

Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells.

Steeg¹,

Moore²,

Oppenheim³

1980

The Journal of Experimental Medicine

151

View full text Add to dashboard Cite

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“…USA 79 (1982) region products from both partners is a priori evidence that these cells are hybrids, we have not formally shown that these are B cell-B cell hybrids. It seems likely that the normal cell in the fusion was a B cell; however, other Ia' cell types are present in spleen [e.g., macrophages (12) or dendritic cells (13)]. …”

mentioning

confidence: 99%

Antigen presentation by Ia+ B cell hybridomas to H-2-restricted T cell hybridomas.

Kappler¹,

White²,

Wegmann³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

226

102

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The Ia+, H-2d BALB/c lymphoma cell line L10.A 2J was fused to T cell-depleted spleen cells from mouse strains bearing other H-2 haplotypes. A portion of the selected hybrids expressed Ia antigens of the normal spleen cell partner in the fusion as evidenced by their presentation of various antigens to a set of antigen-specific I-region-restricted T cell hybridomas. Three cloned hybrids were studied in detail. Antigen presentation was shown to be inhibited specifically by monoclonal anti-Ia antibodies. Both I-A and I-E molecules were expressed, including in the one case examined hybrid I-A and I-E molecules between the H-2d and H-2b haplotypes. These Ia+ B cell hybridomas provide a useful set of tools for studying the role of I-region-encoded molecules in antigen presentation to T cells.

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“…predominate in the peritoneal cavity (1)(2)(3). It has been shown that only the Ia+ subset of macrophages is effective in presenting antigen to T cells (4,5).…”

mentioning

confidence: 99%

Macrophage activation: dissociation of cytotoxic activity from Ia-A antigen expression.

Blumenthal¹,

Roberts²,

Vasil³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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Peritoneal macrophages were obtained from DBA/2 mice that were untreated or after the injection of bacillus Cainette-Guerin (BCG), thioglycollate broth, proteose-peptone broth, or gamma-irradiated P-815 tumor cells. These macrophages were "activated" to become cytotoxic for a fibroblast cell line (L 929) by the addition of lymphokines (LKs), lipopolysaccharide (LPS), or fibroblast interferon , and the expression of I region-associated antigens (Ia-Ad) on the macrophages was examined both before and after activation. Thioglycollateelicited macrophages became Ia-A' when activated by LKs, but they remained la-A-when activated by LPS or IFN-f3. Resident macrophages and proteose-peptone-elicited macrophages remained Ia-A-when activated with LKs. Macrophages from BCGinfected mice were both Ia-A' and cytotoxic for tumor cells without further treatment. In contrast, macrophages from mice injected with gamma-irradiated P-815 mastocytoma cells were Ia-A+ but not cytotoxic, and these macrophages could not be made cytotoxic by incubation with LKs. The cultured macrophage-like cell lines P388D1 and WEHI-3 became Ia-A+ after incubation with LKs, and this treatment amplified the cytotoxicity of both cell lines. We conclude that a number of factors are important in determining whether Ia-A expression accompanies macrophage activation and that Ia-A is irrelevant as a surface marker for macrophage activation.I region-associated antigens (la) have been detected on distinct subpopulations of macrophages. Most macrophages from the spleen, thymus, and liver are la', whereas la-macrophages predominate in the peritoneal cavity (1-3). It has been shown that only the Ia+ subset of macrophages is effective in presenting antigen to T cells (4, 5). In addition, Ia expression appears to be a prerequisite for macrophages to act as accessory cells for lymphokine (LK) production and lymphocyte proliferation (6, 7).Recently, it has been demonstrated that LK preparations can induce la-macrophages to become Ia+ in vitro (8-10). Similar preparations of LKs are known to contain macrophage-activating factor (MAF) which stimulates macrophages to become cytotoxic for tumor cells (11). This raises the questions of whether the Ia' macrophage is the cytotoxic macrophage and whether La antigens can be used as a surface marker for macrophage activation.In this report, we present evidence indicating that the cytotoxic macrophage can be either Ia-A+ or Ia-A-, depending upon the procedures used for elicitation and activation of it. Also, Ia-A was found not to be a useful marker for macrophage activation because Ia-A expression was neither necessary nor sufficient for macrophage cytotoxicity. MATERIALS AND METHODSMice. Our source of peritoneal macrophages was DBA/2 mice that were less than 3 months old (The Jackson Laboratory).Elicitation of Peritoneal Macrophages. Peritoneal exudate cells were collected by lavage with 5 ml of sterile Eagle minimal essential medium. Resident cells were removed from untreated mice. Cells were also obtained (a) 3-4 days ...

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EXPRESSION OF Ia ANTIGENS ON MURINE MACROPHAGES

Cited by 12 publications

References 3 publications

Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells.

Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells.

Antigen presentation by Ia+ B cell hybridomas to H-2-restricted T cell hybridomas.

Macrophage activation: dissociation of cytotoxic activity from Ia-A antigen expression.

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