Expression of <i><scp>HvCslF9</scp></i> and <i><scp>HvCslF6</scp></i> barley genes in the genetic background of wheat and their influence on the wheat β‐glucan content

Cseh, András; Soós, Vilmos; Rakszegi, Marianna; Türkösi, Edina; Balázs, Ervin; Molnár-Láng, Márta

doi:10.1111/aab.12043

Cited by 23 publications

(14 citation statements)

References 37 publications

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“…As mentioned before, (1,3;1,4)-β-glucans are a relatively minor component of durum wheat grain, so investigations of durum genotypes with higher amounts [46][47][48] or wheat-related species such as Aegilops ssp. have been carried out [46,[49][50][51][52][53][54][55] for further genetic-molecular analysis and for the analysis of possible interspecific gene transfer.…”

Section: (13;14)-β-glucan Content and Qtl Regions In Durum Wheatmentioning

confidence: 99%

Non-Starch Polysaccharides in Durum Wheat: A Review

Marcotuli

Colasuonno

Hsieh

et al. 2020

IJMS

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Durum wheat is one of most important cereal crops that serves as a staple dietary component for humans and domestic animals. It provides antioxidants, proteins, minerals and dietary fibre, which have beneficial properties for humans, especially as related to the health of gut microbiota. Dietary fibre is defined as carbohydrate polymers that are non-digestible in the small intestine. However, this dietary component can be digested by microorganisms in the large intestine and imparts physiological benefits at daily intake levels of 30–35 g. Dietary fibre in cereal grains largely comprises cell wall polymers and includes insoluble (cellulose, part of the hemicellulose component and lignin) and soluble (arabinoxylans and (1,3;1,4)-β-glucans) fibre. More specifically, certain components provide immunomodulatory and cholesterol lowering activity, faecal bulking effects, enhanced absorption of certain minerals, prebiotic effects and, through these effects, reduce the risk of type II diabetes, cardiovascular disease and colorectal cancer. Thus, dietary fibre is attracting increasing interest from cereal processors, producers and consumers. Compared with other components of the durum wheat grain, fibre components have not been studied extensively. Here, we have summarised the current status of knowledge on the genetic control of arabinoxylan and (1,3;1,4)-β-glucan synthesis and accumulation in durum wheat grain. Indeed, the recent results obtained in durum wheat open the way for the improvement of these important cereal quality parameters.

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Section: (13;14)-β-glucan Content and Qtl Regions In Durum Wheatmentioning

confidence: 99%

Non-Starch Polysaccharides in Durum Wheat: A Review

Marcotuli

Colasuonno

Hsieh

et al. 2020

IJMS

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“…In rice ( Oryza sativa L.), OsCslF6 knockout mutants synthesise very little (1,3;1,4)-β-glucan (Vega-Sánchez et al 2012 ). In wheat ( Triticum aestivum L.), (1,3;1,4)-β-glucan content is reduced by RNAi inhibition of TaCslF6 (Nemeth et al 2010 ) and is increased by the addition of barley chromosome 7H (on which HvCslF6 is located) (Cseh et al 2013 ). In barley, overexpression of HvCslF6 has been shown to substantially increase (1,3;1,4)-β-glucan content (Burton et al 2011 ), the lack of (1,3;1,4)-β-glucan synthesis in β-glucan-less ( bgl ) barley has been attributed to a specific mutation in a conserved region of HvCslF6 (Taketa et al 2012 ), and reduced (1,3;1,4)-β-glucan has been attributed to an induced mutation in HvCslF6 (Hu et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of the HvCslF6 gene late in grain development may explain quantitative differences in (1,3;1,4)-β-glucan concentration in barley

et al. 2015

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The cellulose synthase-like gene HvCslF6, which is essential for (1,3;1,4)-β-glucan biosynthesis in barley, collocates with quantitative trait loci (QTL) for grain (1,3;1,4)-β-glucan concentration in several populations, including CDC Bold × TR251. Here, an alanine-to-threonine substitution (caused by the only non-synonymous difference between the CDC Bold and TR251 HvCslF6 alleles) was mapped to a position within HvCSLF6 that seems unlikely to affect enzyme stability or function. Consistent with this, transient expression of full-length HvCslF6 cDNAs from CDC Bold and TR251 in Nicotianabenthamiana led to accumulation of similar amounts of (1,3;1,4)-β-glucan accumulation. Monitoring of HvCslF6 transcripts throughout grain development revealed a significant difference late in grain development (more than 30 days after pollination), with TR251 [the parent with higher grain (1,3;1,4)-β-glucan] exhibiting higher transcript levels than CDC Bold. A similar difference was observed between Beka and Logan, the parents of another population in which a QTL had been mapped in the HvCslF6 region. Sequencing of a putative promoter region of HvCslF6 revealed numerous polymorphisms between CDC Bold and TR251, but none between Beka and Logan. While the results of this work indicate that naturally occurring quantitative differences in (1,3;1,4)-β-glucan accumulation may be due to cis-regulated differences in HvCslF6 expression, these could not be attributed to any specific DNA sequence polymorphism. Nevertheless, information on HvCslF6 sequence polymorphism was used to develop molecular markers that could be used in barley breeding to select for the desired [low or high (1,3;1,4)-β-glucan] allele of the QTL.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-015-0208-6) contains supplementary material, which is available to authorized users.

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“…An increased (1,3;1,4)-b-D-glucan level was also detected in the translocation line, demonstrating that the HvCslF6 gene is of potential relevance for the manipulation of wheat (1,3;1,4)-b-D-glucan levels. The Mv9kr1-Igri 1HS ditelosomic and Mv9kr1-Igri 7H disomic wheat/barley addition lines carrying the HvCslF9 and HvCslF6 barley genes, respectively, were used to investigate the additive effect of barley cellulose synthase-like genes on the wheat b-glucan content (Cseh et al 2013). A significantly higher bglucan level was detected in the leaves and grains of the wheat/barley 1HS and 7H addition lines compared to the control wheat line.…”

Section: Characterization and Exploitation Of Wheat-barley Introgressmentioning

confidence: 99%

“…A significantly higher bglucan level was detected in the leaves and grains of the wheat/barley 1HS and 7H addition lines compared to the control wheat line. The expression of the HvCslF9 and HvCslF6 genes in the genetic background of wheat was also determined by quantitative RT-PCR, and the HvCslF9 gene was mapped to the short arm of the 1H chromosome (Cseh et al 2013). The HvGlb1 barley gene, encoding (1,3;1,4)-b-Dglucan endohydrolase isoenzyme EI, is possibly involved in the regulation of the b-glucan level during grain development.…”

Section: Characterization and Exploitation Of Wheat-barley Introgressmentioning

confidence: 99%

Wheat–barley hybridization: the last 40 years

2013

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Several useful alien gene transfers have been reported from related species into wheat (Triticum aestivum), but very few publications have dealt with the development of wheat/barley (Hordeum vulgare) introgression lines. An overview is given here of wheat 9 barley hybridization over the last forty years, including the development of wheat 9 barley hybrids, and of addition and translocation lines with various barley cultivars. A short summary is also given of the wheat 9 barley hybrids produced with other Hordeum species. The meiotic pairing behaviour of wheat 9 barley hybrids is presented, with special regard to the detection of wheatbarley homoeologous pairing using the molecular cytogenetic technique GISH. The effect of in vitro multiplication on the genome composition of intergeneric hybrids is discussed, and the production and characterization of the latest wheat/barley translocation lines are presented. An overview of the agronomical traits (b-glucan content, earliness, salt tolerance, sprouting resistance, etc.) of the newly developed introgression lines is given. The exploitation and possible use of wheat/barley introgression lines for the most up-to-date molecular genetic studies (transcriptome analysis, sequencing of flow-sorted chromosomes) are also discussed.

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Expression of HvCslF9 and HvCslF6 barley genes in the genetic background of wheat and their influence on the wheat β‐glucan content

Cited by 23 publications

References 37 publications

Non-Starch Polysaccharides in Durum Wheat: A Review

Non-Starch Polysaccharides in Durum Wheat: A Review

Differential expression of the HvCslF6 gene late in grain development may explain quantitative differences in (1,3;1,4)-β-glucan concentration in barley

Wheat–barley hybridization: the last 40 years

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